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FGF9 elisa kit :: Human FGF9 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS176006
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $485 +1 FREE 8GB USB
 
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 Product Name   

FGF9, ELISA Kit

★Popular Item★
 Also Known As   

Human FGF9 PicoKine ELISA Kit

 Product Synonym Names    Fibroblast growth factor 9; fibroblast growth factor 9; Elbow knee synostosis; FGF 9; FGF-9; FGF9; FGF9_HUMAN; Fibroblast growth factor 9; GAF; Glia Activating Factor; Glia-activating factor; HBFG 9; HBFG9; HBGF-9; Heparin binding growth factor 9; Heparin-binding growth factor 9; MGC119914; MGC119915; SYNS3
 Product Gene Name   

FGF9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 612961
 3D Structure    ModBase 3D Structure for P31371
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 Species Reactivity    Human
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 Specificity    Natural and recombinant human FGF9
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 Samples    Cell culture supernates, serum and plasma (heparin, EDTA)
 Assay Type    Sandwich
 Detection Range    62.5pg/ml-4000pg/ml
 Sensitivity    < 15pg/ml
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FGF9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FGF9 purchase    MBS176006 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FGF9, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5pg/ml-4000pg/ml in biological research samples containing FGF9, with an estimated sensitivity of < 15pg/ml. The ELISA analytical biochemical technique of the MBS176006 kit is based on FGF9 antibody-FGF9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FGF9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FGF9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FGF9 elisa kit

   Principle of the assay: human FGF9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF9 has been precoated onto 96-well plates. Standards(sf21,M1-S208) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF9 amount of sample captured in plate.
Background: Fibroblast growth factor-9 (FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells.1 The human FGF-9 cDNA cloned by using oligonucleotide probes encodes a polypeptide consisting of 208amino acids. Sequence similarity to other members of the FGF family has been estimated to be around 30%.2FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma.3 FGF9 is produced and secreted by the prostatic stromal cells. It is a potent mitogen for bothprostatic epithelial and stromal cells in culture. FGF9 is an abundant secreted growth factor that can act as both a paracrine mitogen for epithelial cells and an autocrine mitogen for stromal cells. Overexpression of this paracrine and autocrine growth factor may play an important role in the epithelial and stromal proliferation in benign prostatic hyperplasia.4 As a result of glycosylation, the molecular mass is 25-27KDa.
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 Typical Testing Data/Standard Curve (for reference only) of FGF9 elisa kit    FGF9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS176006. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FGF9. It may not necessarily be applicable to this product.
 NCBI GI #    544290
 NCBI GeneID    2254
 NCBI Accession #    P31371.3 [Other Products]
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 UniProt Primary Accession #    P31371 [Other Products]
 UniProt Secondary Accession #    Q3SY32; A8K427 [Other Products]
 UniProt Related Accession #    P31371 [Other Products]
 Molecular Weight    23,441 Da
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 NCBI Official Full Name    Fibroblast growth factor 9
 NCBI Official Synonym Full Names    fibroblast growth factor 9
 NCBI Official Symbol    FGF9 [Similar Products]
 NCBI Official Synonym Symbols   
GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
[Similar Products]
 NCBI Protein Information    fibroblast growth factor 9; heparin-binding growth factor 9; fibroblast growth factor 9 (glia-activating factor)
 UniProt Protein Name    Fibroblast growth factor 9
 UniProt Synonym Protein Names   
Glia-activating factor; GAF; Heparin-binding growth factor 9; HBGF-9
 Protein Family    Fibroblast growth factor
 UniProt Gene Name    FGF9 [Similar Products]
 UniProt Synonym Gene Names    FGF-9; GAF; HBGF-9 [Similar Products]
 UniProt Entry Name    FGF9_HUMAN
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 NCBI Summary for FGF9    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FGF9    FGF9: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3). Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 13q11-q12

Cellular Component: extracellular space; cytoplasm; extracellular region; basement membrane

Molecular Function: heparin binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; embryonic skeletal development; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of activin receptor signaling pathway; substantia nigra development; cell-cell signaling; positive regulation of MAPKKK cascade; protein import into nucleus; male sex determination; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; chondrocyte differentiation; positive regulation of smoothened signaling pathway; angiogenesis; regulation of timing of cell differentiation; embryonic gut development; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; inner ear morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; male gonad development; osteoblast differentiation; positive regulation of cell division; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation

Disease: Multiple Synostoses Syndrome 3
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 Research Articles on FGF9    1. expression is associated with poor prognosis in lung cancer
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FGF9 elisa kitPathways associated with FGF9 elisa kit
 Reference Product  PubMed Publications
 FGFR2 elisa kit  >53 publications with FGF9 and FGFR2
 FGFR3 elisa kit  >50 publications with FGF9 and FGFR3
 FGF10 elisa kit  >39 publications with FGF9 and FGF10
 FGFR1 elisa kit  >36 publications with FGF9 and FGFR1
 FGF7 elisa kit  >27 publications with FGF9 and FGF7
 FGF4 elisa kit  >23 publications with FGF9 and FGF4
 FGFR4 elisa kit  >11 publications with FGF9 and FGFR4
 FGF6 elisa kit  >7 publications with FGF9 and FGF6
 FGF23 elisa kit  >5 publications with FGF9 and FGF23
 FGF19 elisa kit  >3 publications with FGF9 and FGF19
 Products by Pathway  Pathway Diagram
 Activated Point Mutants Of FGFR2 Pathway antibodies  Activated Point Mutants Of FGFR2 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 FGFR Ligand Binding And Activation Pathway antibodies  FGFR Ligand Binding And Activation Pathway Diagram
Diseases associated with FGF9 elisa kitOrgans/Tissues associated with FGF9 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >14 publications with FGF9 and Nervous System Diseases
 Adenocarcinoma Antibodies  >13 publications with FGF9 and Adenocarcinoma
 Lung Diseases Antibodies  >10 publications with FGF9 and Lung Diseases
 Urogenital Abnormalities Antibodies  >9 publications with FGF9 and Urogenital Abnormalities
 Cardiovascular Diseases Antibodies  >8 publications with FGF9 and Cardiovascular Diseases
 Hypertrophy Antibodies  >7 publications with FGF9 and Hypertrophy
 Lung Neoplasms Antibodies  >7 publications with FGF9 and Lung Neoplasms
 Heart Diseases Antibodies  >6 publications with FGF9 and Heart Diseases
 Hyperplasia Antibodies  >5 publications with FGF9 and Hyperplasia
 Carcinoma, Non-Small-Cell Lung Antibodies  >5 publications with FGF9 and Carcinoma, Non-Small-Cell Lung
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >46 publications with FGF9 and Embryonic Tissue
 Brain Antibodies  >36 publications with FGF9 and Brain
 Blood Antibodies  >23 publications with FGF9 and Blood
 Heart Antibodies  >14 publications with FGF9 and Heart
 Eye Antibodies  >12 publications with FGF9 and Eye
 Kidney Antibodies  >12 publications with FGF9 and Kidney
 Connective Tissue Antibodies  >10 publications with FGF9 and Connective Tissue
 Intestine Antibodies  >8 publications with FGF9 and Intestine
 Uterus Antibodies  >7 publications with FGF9 and Uterus
 Thyroid Antibodies  >1 publications with FGF9 and Thyroid
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