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FXYD3 elisa kit :: Human FXYD domain containing ion transport regulator 3 (FXYD3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7247343
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
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 Product Name   

FXYD domain containing ion transport regulator 3 (FXYD3), ELISA Kit

★Popular Item★
 Also Known As   

Human FXYD domain containing ion transport regulator 3 (FXYD3) ELISA Kit

 Product Gene Name   

FXYD3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    604996
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of FXYD3. No significant cross-reactivity or interference between FXYD3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FXYD3 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/ml
 Sensitivity    0.1ng/ml
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FXYD3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FXYD3 purchase    MBS7247343 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FXYD domain containing ion transport regulator 3 (FXYD3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/ml in biological research samples containing FXYD3, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS7247343 kit is based on FXYD3 antibody-FXYD3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FXYD3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FXYD3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FXYD3 elisa kit

   Intended Uses: This FXYD3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of human FXYD3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||FXYD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FXYD3 antibody and an FXYD3-HRP conjugate. The assay sample and buffer are incubated together with FXYD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FXYD3 concentration since FXYD3 from samples and FXYD3-HRP conjugate compete for the anti-FXYD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by FXYD3 from the sample, fewer sites are left to bind FXYD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FXYD3 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for FXYD3 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve of FXYD3 elisa kit    FXYD3 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7247343. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FXYD3. It may not necessarily be applicable to this product.
 NCBI GI #    209862815
 NCBI GeneID    5349
 NCBI Accession #    NP_001129482.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001136010.1 [Other Products]
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 UniProt Secondary Accession #    Q13211; Q6IB59; A6NDE0; C9JDU2; F5H174; F8WB34 [Other Products]
 UniProt Related Accession #    Q14802 [Other Products]
 Molecular Weight    6,718 Da
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 NCBI Official Full Name    FXYD domain-containing ion transport regulator 3 isoform 5
 NCBI Official Synonym Full Names    FXYD domain containing ion transport regulator 3
 NCBI Official Symbol    FXYD3 [Similar Products]
 NCBI Official Synonym Symbols   
MAT8; PLML
[Similar Products]
 NCBI Protein Information    FXYD domain-containing ion transport regulator 3; phospholemman-like protein; mammary tumor 8 kDa protein; chloride conductance inducer protein Mat-8
 UniProt Protein Name    FXYD domain-containing ion transport regulator 3
 UniProt Synonym Protein Names   
Chloride conductance inducer protein Mat-8; Mammary tumor 8 kDa protein; Phospholemman-like
 Protein Family    FXYD domain-containing ion transport regulator
 UniProt Gene Name    FXYD3 [Similar Products]
 UniProt Synonym Gene Names    MAT8; PLML [Similar Products]
 UniProt Entry Name    FXYD3_HUMAN
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 NCBI Summary for FXYD3    This gene belongs to a small family of FXYD-domain containing regulators of Na+/K+ ATPases which share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD, and containing 7 invariant and 6 highly conserved amino acids. This gene encodes a cell membrane protein that may regulate the function of ion-pumps and ion-channels. This gene may also play a role in tumor progression. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]
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 UniProt Comments for FXYD3    FXYD3: Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. May be a modulator capable of activating endogenous oocyte channels. Belongs to the FXYD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane

Molecular Function: chloride channel activity; ATPase binding

Biological Process: regulation of catalytic activity; chloride transport; transmembrane transport
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 Research Articles on FXYD3    1. ExoS facilitates P. aeruginosa penetration through the intestinal epithelial barrier by binding to FXYD3 and thereby impairing the defense function of tight junctions against bacterial penetration.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FXYD3 elisa kitPathways associated with FXYD3 elisa kit
 Reference Product  PubMed Publications
 FXYD5 elisa kit  >3 publications with FXYD3 and FXYD5
 AGR2 elisa kit  >2 publications with FXYD3 and AGR2
 CDH1 elisa kit  >1 publications with FXYD3 and CDH1
 Products by Pathway  Pathway Diagram
 Ion Channel Transport Pathway antibodies  Ion Channel Transport Pathway Diagram
 Ion Transport By P-type ATPases Pathway antibodies  Ion Transport By P-type ATPases Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
Diseases associated with FXYD3 elisa kitOrgans/Tissues associated with FXYD3 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >12 publications with FXYD3 and Carcinoma
 Breast Neoplasms Antibodies  >6 publications with FXYD3 and Breast Neoplasms
 Adenocarcinoma Antibodies  >5 publications with FXYD3 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >5 publications with FXYD3 and Neoplasm Metastasis
 Urinary Bladder Neoplasms Antibodies  >4 publications with FXYD3 and Urinary Bladder Neoplasms
 Pancreatic Neoplasms Antibodies  >3 publications with FXYD3 and Pancreatic Neoplasms
 Liver Neoplasms Antibodies  >2 publications with FXYD3 and Liver Neoplasms
 Necrosis Antibodies  >2 publications with FXYD3 and Necrosis
 Nervous System Diseases Antibodies  >1 publications with FXYD3 and Nervous System Diseases
 Lung Neoplasms Antibodies  >1 publications with FXYD3 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >8 publications with FXYD3 and Muscle
 Intestine Antibodies  >6 publications with FXYD3 and Intestine
 Heart Antibodies  >5 publications with FXYD3 and Heart
 Bladder Antibodies  >5 publications with FXYD3 and Bladder
 Stomach Antibodies  >5 publications with FXYD3 and Stomach
 Brain Antibodies  >5 publications with FXYD3 and Brain
 Kidney Antibodies  >4 publications with FXYD3 and Kidney
 Nerve Antibodies  >3 publications with FXYD3 and Nerve
 Ovary Antibodies  >2 publications with FXYD3 and Ovary
 Blood Antibodies  >2 publications with FXYD3 and Blood
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