Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow FAM3B rightarrow Human FAM3B  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Tetanus Toxin, Clostridium tetani Antibody
 • GFAP Blocking Peptide
 • Syphilis Treponema pallidum TP17 Recombinant Protein
 • Histidine Triad Nucleotide Binding Protein 1 (HINT1) Recombinant Protein
 • VEGF Antibody
 • ABCA13 Antibody
 • ADAM metallopeptidase domain 28 (ADAM28) ELISA Kit
 • SYVN1 - HRD1 Antibody
 • Rabbit anti Monkey IgG Fab Secondary Antibody
 • Granulocyte Chemotactic Protein 2 (GCP2) ELISA Kit
 • pregnancy specific beta-1-glycoprotein 5 (PSG5) ELISA Kit
 • apolipoprotein A-V (APOA5) ELISA Kit
 • SNAI2 - SLUG Antibody
 • PAPSS1 Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

FAM3B elisa kit :: Human Family With Sequence Similarity 3, Member B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS750185
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Family With Sequence Similarity 3, Member B (FAM3B), ELISA Kit

★Popular Item★
 Also Known As   

Human Family With Sequence Similarity 3, Member B ELISA Kit

 Product Gene Name   

FAM3B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    608617
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of FAM3B. No significant cross-reactivity or interference between FAM3B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FAM3B and all the analogues, therefore, cross reaction may still exist in some cases.
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1g/mL
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FAM3B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FAM3B purchase    MBS750185 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Family With Sequence Similarity 3, Member B (FAM3B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing FAM3B, with an estimated sensitivity of 0.1g/mL. The ELISA analytical biochemical technique of the MBS750185 kit is based on FAM3B antibody-FAM3B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FAM3B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FAM3B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for FAM3B elisa kit

   Intended Uses: This FAM3B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FAM3B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: FAM3B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FAM3B antibody and an FAM3B-HRP conjugate. The assay sample and buffer are incubated together with FAM3B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FAM3B concentration since FAM3B from samples and FAM3B-HRP conjugate compete for the anti-FAM3B antibody binding site. Since the number of sites is limited, as more sites are occupied by FAM3B from the sample, fewer sites are left to bind FAM3B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAM3B concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for FAM3B elisa kit    Cell Biology
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of FAM3B elisa kit    FAM3B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS750185. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for FAM3B. It may not necessarily be applicable to this product.
 NCBI GI #    37182042
 NCBI Accession #    AAQ88824.1 [Other Products]
 Molecular Weight    20,815 Da
Table BarTOPTable Bar
 NCBI Official Full Name    FAM3B
 UniProt Protein Name    Protein FAM3B
 UniProt Synonym Protein Names   
Cytokine-like protein 2-21; Pancreatic-derived factor; PANDER
 Protein Family    Protein
 UniProt Gene Name    FAM3B [Similar Products]
 UniProt Synonym Gene Names    C21orf11; C21orf76; PRED44; UNQ320/PRO365; PANDER [Similar Products]
 UniProt Entry Name    FAM3B_HUMAN
Section Bar
 UniProt Comments for FAM3B    FAM3B: Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. Present in insulin secretory granules and likely cosecreted with insulin. Belongs to the FAM3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear envelope; Cytokine; Apoptosis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space; extracellular region

Molecular Function: cytokine activity

Biological Process: insulin secretion; apoptosis
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions