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FCN2 elisa kit :: Human Ficolin-2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS177113
 Typical Testing Data/Standard Curve
Unit / Price
96-Strip-Wells  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ficolin-2 (FCN2), ELISA Kit

★Popular Item★
 Also Known As   

Human Ficolin-2 PicoKine ELISA Kit

 Product Synonym Names    Ficolin-2; ficolin (collagen/fibrinogen domain containing lectin) 2; 37 kDa elastin-binding protein; Collagen/fibrinogen domain containing protein 2; Collagen/fibrinogen domain-containing protein 2; EBP 37; EBP-37; EBP37; FCN 2; Fcn2; FCN2_HUMAN; FCNL; Ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin); Ficolin B; Ficolin beta; Ficolin-2; Ficolin-B; Ficolin-beta; Ficolin2; Hucolin; L ficolin; L-ficolin; OTTHUMP00000022518; P35; RP11 263F14.2; Serum lectin p35
 Product Gene Name   

FCN2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601624
 3D Structure    ModBase 3D Structure for Q15485
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 Species Reactivity    Human
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 Specificity    Natural and recombinant human Ficolin-2
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 Samples    Cell culture supernates, serum and plasma(heparin).
 Assay Type    Sandwich
 Detection Range    0.78ng/ml-50ng/ml
 Sensitivity    < 20pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FCN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FCN2 purchase    MBS177113 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ficolin-2 (FCN2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78ng/ml-50ng/ml in biological research samples containing FCN2, with an estimated sensitivity of < 20pg/ml. The ELISA analytical biochemical technique of the MBS177113 kit is based on FCN2 antibody-FCN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FCN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FCN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma(heparin). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for FCN2 elisa kit

   Principle of the Assay: The nL;man Ficolin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay :echno;ogy. A monoclonal antibody from mouse specific for Ficolin-2 has been precoated onto 96-well plates. Standards(NSO. L26-A313) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-2 amount of sample captured in plate.

Background/Introduction: Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide. a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.
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  Typical Testing Data/Standard Curve of FCN2 elisa kit    Human Ficolin-2 PicoKine ELISA Kit standard curve
FCN2 elisa kit  Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS177113. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FCN2. It may not necessarily be applicable to this product.
 NCBI GI #    166214934
 NCBI GeneID    2220
 NCBI Accession #    Q15485.2 [Other Products]
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 UniProt Primary Accession #    Q15485 [Other Products]
 UniProt Secondary Accession #    Q6IS69; Q7M4P4; Q9UC57; A6NFG7; A8K478 [Other Products]
 UniProt Related Accession #    Q15485 [Other Products]
 Molecular Weight    30,228 Da
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 NCBI Official Full Name    Ficolin-2
 NCBI Official Synonym Full Names    ficolin (collagen/fibrinogen domain containing lectin) 2
 NCBI Official Symbol    FCN2 [Similar Products]
 NCBI Official Synonym Symbols   
P35; FCNL; EBP-37; ficolin-2
[Similar Products]
 NCBI Protein Information    ficolin-2; L-ficolin; ficolin B; ficolin-B; ficolin-beta; serum lectin p35; 37 kDa elastin-binding protein; collagen/fibrinogen domain-containing protein 2; ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
 UniProt Protein Name    Ficolin-2
 UniProt Synonym Protein Names   
37 kDa elastin-binding protein; Collagen/fibrinogen domain-containing protein 2; EBP-37; Ficolin-B; Ficolin-beta; Hucolin; L-ficolin; Serum lectin p35
 Protein Family    Ficolin
 UniProt Gene Name    FCN2 [Similar Products]
 UniProt Synonym Gene Names    FCNL [Similar Products]
 UniProt Entry Name    FCN2_HUMAN
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 NCBI Summary for FCN2    The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FCN2    FCN2: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. Belongs to the ficolin lectin family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 9q34
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 Research Articles on FCN2    1. Data suggest that the hepatitis C virus (HCV) entry inhibitor ficolin-2 is an antiviral innate immune molecule, whereas apolipoprotein E3 (ApoE3) blocks the effect of ficolin-2 and mediates an immune escape mechanism during chronic HCV infection.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FCN2 elisa kitPathways associated with FCN2 elisa kit
 Reference Product  PubMed Publications
 MBL2 elisa kit  >16 publications with FCN2 and MBL2
 MASP2 elisa kit  >5 publications with FCN2 and MASP2
 CRP elisa kit  >1 publications with FCN2 and CRP
 MASP1 elisa kit  >1 publications with FCN2 and MASP1
 Products by Pathway  Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Creation Of C4 And C2 Activators Pathway antibodies  Creation Of C4 And C2 Activators Pathway Diagram
 Ficolins Bind To Repetitive Carbohydrate Structures On The Target Cell Surface Pathway antibodies  Ficolins Bind To Repetitive Carbohydrate Structures On The Target Cell Surface Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Initial Triggering Of Complement Pathway antibodies  Initial Triggering Of Complement Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Lectin Pathway Of Complement Activation antibodies  Lectin Pathway Of Complement Activation Diagram
Diseases associated with FCN2 elisa kitOrgans/Tissues associated with FCN2 elisa kit
 Disease Name  Pubmed Publications
 Hepatitis Antibodies  >3 publications with FCN2 and Hepatitis
 Inflammation Antibodies  >3 publications with FCN2 and Inflammation
 Liver Diseases Antibodies  >2 publications with FCN2 and Liver Diseases
 Lung Diseases Antibodies  >2 publications with FCN2 and Lung Diseases
 Urologic Diseases Antibodies  >1 publications with FCN2 and Urologic Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with FCN2 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >19 publications with FCN2 and Blood
 Liver Antibodies  >5 publications with FCN2 and Liver
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