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FUCa1 elisa kit :: Human Fucosidase, Alpha L1, Tissue ELISA Kit

Scan QR to view Datasheet Catalog #    MBS732061
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Fucosidase, Alpha L1, Tissue (FUCa1), ELISA Kit

★Popular Item★
 Also Known As   

Human Fucosidase, Alpha L1, Tissue ELISA Kit

 Product Gene Name   

FUCa1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    612280
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of AFU. No significant cross-reactivity or interference between AFU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AFU and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This AFU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AFU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FUCa1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FUCa1 purchase    MBS732061 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fucosidase, Alpha L1, Tissue (FUCa1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing FUCa1, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS732061 kit is based on FUCa1 antibody-FUCa1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FUCa1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FUCa1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FUCa1 elisa kit

   Principle of the assay: AFU ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AFU. Standards or samples are then added to the microtiter plate wells and AFU if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AFU present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AFU are added to each well to “sandwich” the AFU immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AFU and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFU concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for FUCa1 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of FUCa1 elisa kit    FUCa1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS732061. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FUCa1. It may not necessarily be applicable to this product.
 NCBI GI #    119615504
 NCBI GeneID    2517
 NCBI Accession #    EAW95098.1 [Other Products]
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 UniProt Secondary Accession #    Q14334; Q14335; Q3LID0; Q8NAC2; B2RBG3 [Other Products]
 UniProt Related Accession #    P04066 [Other Products]
 Molecular Weight    53,689 Da
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 NCBI Official Full Name    fucosidase, alpha-L- 1, tissue
 NCBI Official Synonym Full Names    fucosidase, alpha-L- 1, tissue
 NCBI Official Symbol    FUCA1 [Similar Products]
 NCBI Official Synonym Symbols   
FUCA
[Similar Products]
 NCBI Protein Information    tissue alpha-L-fucosidase; alpha-L-fucosidase 1; alpha-L-fucosidase I; alpha-L-fucoside fucohydrolase 1
 UniProt Protein Name    Tissue alpha-L-fucosidase
 UniProt Synonym Protein Names   
Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; Alpha-L-fucosidase 1
 Protein Family    Tissue alpha-L-fucosidase
 UniProt Gene Name    FUCA1 [Similar Products]
 UniProt Synonym Gene Names    Nbla10230; Alpha-L-fucosidase 1 [Similar Products]
 UniProt Entry Name    FUCO_HUMAN
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 NCBI Summary for FUCa1    The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]
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 UniProt Comments for FUCa1    FUCA1: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins. Defects in FUCA1 are the cause of fucosidosis (FUCA1D). FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. Belongs to the glycosyl hydrolase 29 family.

Protein type: Hydrolase; EC 3.2.1.51; Glycan Metabolism - other glycan degradation

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: lysosome; cytoplasm

Molecular Function: alpha-L-fucosidase activity; fucose binding

Biological Process: fucose metabolic process; glycosaminoglycan catabolic process; glycoside catabolic process

Disease: Fucosidosis
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 Research Articles on FUCa1    1. Preoperative serum AFU is a prognostic predictor of hepatocellular carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FUCa1 elisa kitPathways associated with FUCa1 elisa kit
 Reference Product  PubMed Publications
 PGM1 elisa kit  >2 publications with FUCa1 and PGM1
 PGD elisa kit  >1 publications with FUCa1 and PGD
 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Other Glycan Degradation Pathway antibodies  Other Glycan Degradation Pathway Diagram
 Other Glycan Degradation Pathway antibodies  Other Glycan Degradation Pathway Diagram
Diseases associated with FUCa1 elisa kitOrgans/Tissues associated with FUCa1 elisa kit
 Disease Name  Pubmed Publications
 Fucosidosis Antibodies  >7 publications with FUCa1 and Fucosidosis
 Neoplasm Metastasis Antibodies  >3 publications with FUCa1 and Neoplasm Metastasis
 Adenocarcinoma Antibodies  >2 publications with FUCa1 and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >1 publications with FUCa1 and Carcinoma, Squamous Cell
 Disease Models, Animal Antibodies  >1 publications with FUCa1 and Disease Models, Animal
 Liver Neoplasms Antibodies  >1 publications with FUCa1 and Liver Neoplasms
 Cardiovascular Diseases Antibodies  >1 publications with FUCa1 and Cardiovascular Diseases
 Hemorrhage Antibodies  >1 publications with FUCa1 and Hemorrhage
 Liver Diseases Antibodies  >1 publications with FUCa1 and Liver Diseases
 Inflammation Antibodies  >1 publications with FUCa1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >15 publications with FUCa1 and Blood
 Liver Antibodies  >2 publications with FUCa1 and Liver
 Intestine Antibodies  >2 publications with FUCa1 and Intestine
 Lymph Node Antibodies  >1 publications with FUCa1 and Lymph Node
 Skin Antibodies  >1 publications with FUCa1 and Skin
 Bone Antibodies  >1 publications with FUCa1 and Bone
 Lung Antibodies  >1 publications with FUCa1 and Lung
 Placenta Antibodies  >1 publications with FUCa1 and Placenta
 Kidney Antibodies  >1 publications with FUCa1 and Kidney
 Lymph Antibodies  >1 publications with FUCa1 and Lymph
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