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CSF3 elisa kit :: Human G-CSF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175868
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $305 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

G-CSF (CSF3), ELISA Kit

★Popular Item★
 Also Known As   

Human G-CSF PicoKine ELISA Kit

 Product Synonym Names    Granulocyte colony-stimulating factor; colony stimulating factor 3 (granulocyte); C17orf33; Colony stimulating factor 3 (granulocyte); CSF 3; CSF beta; CSF3; CSF3OS; Csfg; Filgrastim; GCSA; GCSF; Granulocyte colony stimulating fact; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; MGI IG; OTTMUSP00000006785; Pluripoietin; RP23-395E10.4
 Product Gene Name   

CSF3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    138970
 3D Structure    ModBase 3D Structure for P09919
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 Species Reactivity    Human
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 Specificity    Natural and recombinant human G-CSF
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 Samples    Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
 Assay Type    Sandwich
 Detection Range    31.2pg/ml-2000pg/ml
 Sensitivity    < 4pg/ml
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CSF3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CSF3 purchase    MBS175868 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the G-CSF (CSF3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2pg/ml-2000pg/ml in biological research samples containing CSF3, with an estimated sensitivity of < 4pg/ml. The ELISA analytical biochemical technique of the MBS175868 kit is based on CSF3 antibody-CSF3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CSF3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CSF3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin, EDTA, citrate). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CSF3 elisa kit

   Principle of the assay: human G-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for G-CSF has been precoated onto 96-well plates. Standards(E.coli,T31-P204) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for G-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human G-CSF amount of sample captured in plate.
Background: Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture.1 G-CSF is also termed colonystimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity.2 Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Statpathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.3 The standard product used in this kit is recombinant humanG-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.
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 Typical Testing Data/Standard Curve (for reference only) of CSF3 elisa kit    Human G-CSF PicoKine ELISA Kit standard curve
CSF3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS175868. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CSF3. It may not necessarily be applicable to this product.
 NCBI GI #    117564
 NCBI GeneID    1440
 NCBI Accession #    P09919.1 [Other Products]
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 UniProt Primary Accession #    P09919 [Other Products]
 UniProt Secondary Accession #    A8MXR7 [Other Products]
 UniProt Related Accession #    P09919 [Other Products]
 Molecular Weight    18,434 Da
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 NCBI Official Full Name    Granulocyte colony-stimulating factor
 NCBI Official Synonym Full Names    colony stimulating factor 3 (granulocyte)
 NCBI Official Symbol    CSF3 [Similar Products]
 NCBI Official Synonym Symbols   
GCSF; CSF3OS; C17orf33
[Similar Products]
 NCBI Protein Information    granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin
 UniProt Protein Name    Granulocyte colony-stimulating factor
 UniProt Synonym Protein Names   
Pluripoietin; INN: Filgrastim; INN: Lenograstim
 Protein Family    Granulocyte colony-stimulating factor
 UniProt Gene Name    CSF3 [Similar Products]
 UniProt Synonym Gene Names    C17orf33; GCSF; G-CSF [Similar Products]
 UniProt Entry Name    CSF3_HUMAN
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 NCBI Summary for CSF3    The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
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 UniProt Comments for CSF3    G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space

Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding

Biological Process: positive regulation of protein binding; positive regulation of myeloid cell differentiation; granulocyte differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter
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 Research Articles on CSF3    1. SCF+G-CSF treatment in chronic stroke remodels neural circuits in the aged brain
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CSF3 elisa kitPathways associated with CSF3 elisa kit
 Reference Product  PubMed Publications
 CSF3R elisa kit  >13 publications with CSF3 and CSF3R
 MMP9 elisa kit  >7 publications with CSF3 and MMP9
 IL10 elisa kit  >6 publications with CSF3 and IL10
 CXCL12 elisa kit  >3 publications with CSF3 and CXCL12
 ERBB2 elisa kit  >2 publications with CSF3 and ERBB2
 STAT3 elisa kit  >2 publications with CSF3 and STAT3
 SELL elisa kit  >1 publications with CSF3 and SELL
 CXCR4 elisa kit  >1 publications with CSF3 and CXCR4
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
Diseases associated with CSF3 elisa kitOrgans/Tissues associated with CSF3 elisa kit
 Disease Name  Pubmed Publications
 Disease Susceptibility Antibodies  >6 publications with CSF3 and Disease Susceptibility
 Nervous System Diseases Antibodies  >5 publications with CSF3 and Nervous System Diseases
 Neutropenia Antibodies  >3 publications with CSF3 and Neutropenia
 Breast Neoplasms Antibodies  >2 publications with CSF3 and Breast Neoplasms
 Leukopenia Antibodies  >2 publications with CSF3 and Leukopenia
 Anemia Antibodies  >2 publications with CSF3 and Anemia
 Leukemia, Myeloid, Acute Antibodies  >2 publications with CSF3 and Leukemia, Myeloid, Acute
 Peripheral Nervous System Diseases Antibodies  >1 publications with CSF3 and Peripheral Nervous System Diseases
 Pulmonary Fibrosis Antibodies  >1 publications with CSF3 and Pulmonary Fibrosis
 Brain Injuries Antibodies  >1 publications with CSF3 and Brain Injuries
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >7 publications with CSF3 and Skin
 Lung Antibodies  >5 publications with CSF3 and Lung
 Muscle Antibodies  >4 publications with CSF3 and Muscle
 Liver Antibodies  >2 publications with CSF3 and Liver
 Brain Antibodies  >2 publications with CSF3 and Brain
 Uterus Antibodies  >2 publications with CSF3 and Uterus
 Uncharacterized Tissue Antibodies  >1 publications with CSF3 and Uncharacterized Tissue
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