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GPR172A elisa kit :: Human G protein-coupled receptor 172A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702260
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

G protein-coupled receptor 172A (GPR172A), ELISA Kit

★Popular Item★
 Also Known As   

Human riboflavin transporter 3, RFT3 ELISA Kit

 Product Synonym Names    Human riboflavin transporter 3 (RFT3) ELISA Kit; D15Ertd747e; GPCR; GPCR41; PAR1; putative G-protein coupled receptor GPCR41; G protein-coupled receptor 172A
 Product Gene Name   

GPR172A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702260 Testing Data    Testing Data PDF
 OMIM    gene 614707
 3D Structure    ModBase 3D Structure for Q9HAB3
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human GPR172A. No significant cross-reactivity or interference between Human GPR172A and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml-2000 pg/ml
 Sensitivity    7.81 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Transport
 Protein Biological Process 3    Transport
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GPR172A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GPR172A purchase    MBS702260 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the G protein-coupled receptor 172A (GPR172A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing GPR172A, with an estimated sensitivity of 7.81 pg/ml. The ELISA analytical biochemical technique of the MBS702260 kit is based on GPR172A antibody-GPR172A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPR172A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPR172A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for GPR172A elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RFT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RFT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RFT3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RFT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of GPR172A elisa kit    GPR172A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702260. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GPR172A. It may not necessarily be applicable to this product.
 NCBI GI #    359465547
 NCBI GeneID    79581
 NCBI Accession #    NP_001240744.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001253815.1 [Other Products]
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 UniProt Primary Accession #    Q9HAB3 [Other Products]
 UniProt Secondary Accession #    Q86UT1; A8K6B6; D3DWL8; G1UCY1 [Other Products]
 UniProt Related Accession #    Q9HAB3 [Other Products]
 Molecular Weight    45,777 Da
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 NCBI Official Full Name    solute carrier family 52, riboflavin transporter, member 2
 NCBI Official Synonym Full Names    solute carrier family 52 (riboflavin transporter), member 2
 NCBI Official Symbol    SLC52A2 [Similar Products]
 NCBI Official Synonym Symbols   
PAR1; RFT3; RFVT2; hRFT3; BVVLS2; GPCR41; GPR172A; D15Ertd747e
[Similar Products]
 NCBI Protein Information    solute carrier family 52, riboflavin transporter, member 2; PERV-A receptor 1; riboflavin transporter 3; G protein-coupled receptor 172A; porcine endogenous retrovirus A receptor 1; putative G-protein coupled receptor GPCR41
 UniProt Protein Name    Solute carrier family 52, riboflavin transporter, member 2
 UniProt Synonym Protein Names   
Porcine endogenous retrovirus A receptor 1; PERV-A receptor 1; Protein GPR172A; Riboflavin transporter 3; hRFT3
 UniProt Gene Name    SLC52A2 [Similar Products]
 UniProt Synonym Gene Names    GPR172A; PAR1; RFT3; PERV-A receptor 1; hRFT3 [Similar Products]
 UniProt Entry Name    S52A2_HUMAN
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 NCBI Summary for GPR172A    This gene encodes a membrane protein which belongs to the riboflavin transporter family. In humans, riboflavin must be obtained by intestinal absorption because it cannot be synthesized by the body. The water-soluble vitamin riboflavin is processed to the coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) which then act as intermediaries in many cellular metabolic reactions. Paralogous members of the riboflavin transporter gene family are located on chromosomes 17 and 20. Unlike other members of this family, this gene has higher expression in brain tissue than small intestine. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. Mutations in this gene have been associated with Brown-Vialetto-Van Laere syndrome 2 - an autosomal recessive progressive neurologic disorder characterized by deafness, bulbar dysfunction, and axial and limb hypotonia. [provided by RefSeq, Jul 2012]
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 UniProt Comments for GPR172A    Function: Riboflavin transporter. Riboflavin transport is Na+-independent but moderately pH-sensitive. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin. Weakly inhibited by flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN). In case of infection by retroviruses, acts as a cell receptor to retroviral envelopes similar to the porcine endogenous retrovirus (PERV-A).3 PublicationsManual assertion based on experiment in:Ref.1
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 Research Articles on GPR172A    1. data suggest that MMND is a distinct clinical subgroup of childhood onset MND patients where the known genetic defects are so far negative.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with GPR172A elisa kitOrgans/Tissues associated with GPR172A elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >9 publications with GPR172A and Nervous System Diseases
 Respiratory Insufficiency Antibodies  >1 publications with GPR172A and Respiratory Insufficiency
 Brain Diseases Antibodies  >1 publications with GPR172A and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with GPR172A and Blood
 Brain Antibodies  >1 publications with GPR172A and Brain
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