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GAD elisa kit :: Human GAD ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2502500 GAD elisa kit
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GAD, ELISA Kit

★Popular Item★
 Also Known As   

Human GAD (Glutamic Acid Decarboxylase) ELISA Kit

 Product Gene Name   

GAD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2502500 COA    COA PDF
 OMIM    603513
 3D Structure    ModBase 3D Structure for Q99259
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human GAD. No significant cross-reactivity or interference between Human GAD and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.781-50ng/mL
 Sensitivity    0.469ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GAD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GAD purchase    MBS2502500 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GAD, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.781-50ng/mL in biological research samples containing GAD, with an estimated sensitivity of 0.469ng/mL. The ELISA analytical biochemical technique of the MBS2502500 kit is based on GAD antibody-GAD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GAD elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GAD concentrations in serum, plasma and other biological fluids.

Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GAD. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GAD and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GAD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GAD. You can calculate the concentration of GAD in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2502500. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GAD. It may not necessarily be applicable to this product.
 NCBI GI #    1247492
 NCBI GeneID    2571
 NCBI Accession #    CAA01913.1 [Other Products]
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 UniProt Primary Accession #    Q99259 [Other Products]
 UniProt Secondary Accession #    Q49AK1; Q53TQ7; Q9BU91; Q9UHH4 [Other Products]
 UniProt Related Accession #    Q99259 [Other Products]
 Molecular Weight    47,440 Da
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 NCBI Official Full Name    GAD
 NCBI Official Synonym Full Names    glutamate decarboxylase 1 (brain, 67kDa)
 NCBI Official Symbol    GAD1 [Similar Products]
 NCBI Official Synonym Symbols   
GAD; SCP; CPSQ1
[Similar Products]
 NCBI Protein Information    glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD-67; glutamate decarboxylase 67 kDa isoform
 UniProt Protein Name    Glutamate decarboxylase 1
 UniProt Synonym Protein Names   
67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
 Protein Family    D-gluconate dehydratase
 UniProt Gene Name    GAD1 [Similar Products]
 UniProt Synonym Gene Names    GAD; GAD67; GAD-67 [Similar Products]
 UniProt Entry Name    DCE1_HUMAN
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 NCBI Summary for GAD    This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GAD    GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 4.1.1.15; Lyase; Amino Acid Metabolism - alanine, aspartate and glutamate; Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: plasma membrane; vesicle membrane; intracellular

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate catabolic process; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process

Disease: Cerebral Palsy, Spastic Quadriplegic, 1
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 Research Articles on GAD    1. CNR1, GAD1 and BDNF polymorphisms are associated with male heroin dependence in the Dai population in China.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GAD elisa kitPathways associated with GAD elisa kit
 Reference Product  PubMed Publications
 GAD2 elisa kit  >50 publications with GAD and GAD2
 GLUL elisa kit  >2 publications with GAD and GLUL
 OLIG2 elisa kit  >2 publications with GAD and OLIG2
 ABAT elisa kit  >1 publications with GAD and ABAT
 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
Diseases associated with GAD elisa kitOrgans/Tissues associated with GAD elisa kit
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >56 publications with GAD and Mental Disorders
 Schizophrenia Antibodies  >41 publications with GAD and Schizophrenia
 Nervous System Diseases Antibodies  >29 publications with GAD and Nervous System Diseases
 Brain Diseases Antibodies  >23 publications with GAD and Brain Diseases
 Disease Models, Animal Antibodies  >15 publications with GAD and Disease Models, Animal
 Seizures Antibodies  >9 publications with GAD and Seizures
 Anxiety Disorders Antibodies  >8 publications with GAD and Anxiety Disorders
 Autistic Disorder Antibodies  >6 publications with GAD and Autistic Disorder
 Depressive Disorder Antibodies  >6 publications with GAD and Depressive Disorder
 Cognition Disorders Antibodies  >4 publications with GAD and Cognition Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >103 publications with GAD and Brain
 Embryonic Tissue Antibodies  >2 publications with GAD and Embryonic Tissue
 Muscle Antibodies  >2 publications with GAD and Muscle
 Eye Antibodies  >2 publications with GAD and Eye
 Bone Antibodies  >1 publications with GAD and Bone
 Thymus Antibodies  >1 publications with GAD and Thymus
 Uncharacterized Tissue Antibodies  >1 publications with GAD and Uncharacterized Tissue
 Intestine Antibodies  >1 publications with GAD and Intestine
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