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GZMK elisa kit :: Human GZMK ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2512767
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GZMK, ELISA Kit

★Popular Item★
 Also Known As   

Human GZMK (Granzyme K) ELISA Kit

 Product Gene Name   

GZMK elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600784
 3D Structure    ModBase 3D Structure for P49863
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human GZMK. No significant cross-reactivity or interference between Human GZMK and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GZMK and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    31.25-2000pg/mL
 Sensitivity    Min: 18.75pg/mL; Max: 2000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GZMK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GZMK purchase    MBS2512767 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GZMK, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing GZMK, with an estimated sensitivity of Min: 18.75pg/mL; Max: 2000pg/mL. The ELISA analytical biochemical technique of the MBS2512767 kit is based on GZMK antibody-GZMK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GZMK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GZMK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GZMK elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GZMK in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GZMK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GZMK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GZMK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GZMK. You can calculate the concentration of Human GZMK in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of GZMK elisa kit    GZMK elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2512767. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GZMK. It may not necessarily be applicable to this product.
 NCBI GI #    4504235
 NCBI GeneID    3003
 NCBI Accession #    NP_002095.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002104.2 [Other Products]
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 UniProt Primary Accession #    P49863 [Other Products]
 UniProt Secondary Accession #    B2R563 [Other Products]
 UniProt Related Accession #    P49863 [Other Products]
 Molecular Weight    28,882 Da
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 NCBI Official Full Name    granzyme K
 NCBI Official Synonym Full Names    granzyme K (granzyme 3; tryptase II)
 NCBI Official Symbol    GZMK [Similar Products]
 NCBI Official Synonym Symbols   
TRYP2
[Similar Products]
 NCBI Protein Information    granzyme K; NK-Tryp-2; NK-tryptase-2; fragmentin-3; granzyme 3; granzyme K (serine protease, granzyme 3; tryptase II); granzyme-3
 UniProt Protein Name    Granzyme K
 UniProt Synonym Protein Names   
Fragmentin-3; Granzyme-3; NK-tryptase-2; NK-Tryp-2
 Protein Family    Granzyme
 UniProt Gene Name    GZMK [Similar Products]
 UniProt Synonym Gene Names    TRYP2; NK-Tryp-2 [Similar Products]
 UniProt Entry Name    GRAK_HUMAN
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 NCBI Summary for GZMK    This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GZMK    GZMK: this protein is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide; EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis
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 Research Articles on GZMK    1. Extracellular GrK is an unexpected direct modulator of lipopolysaccharide-TLR4 signaling during the antimicrobial innate immune response.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GZMK elisa kitDiseases associated with GZMK elisa kit
 Reference Product  PubMed Publications
 GZMB elisa kit  >36 publications with GZMK and GZMB
 GZMA elisa kit  >12 publications with GZMK and GZMA
 PRF1 elisa kit  >9 publications with GZMK and PRF1
 GZMH elisa kit  >2 publications with GZMK and GZMH
 CCR5 elisa kit  >1 publications with GZMK and CCR5
 CD2 elisa kit  >1 publications with GZMK and CD2
 Disease Name  Pubmed Publications
 Death Antibodies  >14 publications with GZMK and Death
 Inflammation Antibodies  >4 publications with GZMK and Inflammation
 Lung Diseases Antibodies  >1 publications with GZMK and Lung Diseases
 Heart Diseases Antibodies  >1 publications with GZMK and Heart Diseases
 Depressive Disorder Antibodies  >1 publications with GZMK and Depressive Disorder
 Necrosis Antibodies  >1 publications with GZMK and Necrosis
Organs/Tissues associated with GZMK elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >26 publications with GZMK and Blood
 Lung Antibodies  >3 publications with GZMK and Lung
 Brain Antibodies  >1 publications with GZMK and Brain
 Thymus Antibodies  >1 publications with GZMK and Thymus
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