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GALC elisa kit :: Human Galactocerebrosidase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS760168
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Galactocerebrosidase (GALC), ELISA Kit

★Popular Item★
 Also Known As   

Human Galactocerebrosidase ELISA Kit

 Product Synonym Names    GALC/Galactocerebrosidase/Galactocerebroside beta-galactosidase/Galactosylceraminidase/galactosylceramidase (Krabbe disease)/Galactosylceramide beta-galactosidase/GALCERase
 Product Gene Name   

GALC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS760168 COA    COA PDF
 OMIM    245200
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of GALC . No significant cross-reactivity or interference between GALC and analogues was observed.
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 Samples    Cells
 Assay Type    Sandwich
 Detection Range    78-5000pg/ml
 Sensitivity    <46.9pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GALC were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GALC were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GALC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GALC purchase    MBS760168 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Galactocerebrosidase (GALC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78-5000pg/ml in biological research samples containing GALC, with an estimated sensitivity of <46.9pg/ml. The ELISA analytical biochemical technique of the MBS760168 kit is based on GALC antibody-GALC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GALC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GALC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cells. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GALC elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GALC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- GALC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GALC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GALC can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of GALC elisa kit    GALC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS760168. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GALC. It may not necessarily be applicable to this product.
 NCBI GI #    319655717
 NCBI GeneID    2581
 NCBI Accession #    NP_001188330.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001201401.1 [Other Products]
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 UniProt Secondary Accession #    Q8J030; B4DKE8; B4DYN1; B4DZJ8; B7Z7Z2; J3KN25; J3KPP8 [Other Products]
 UniProt Related Accession #    P54803 [Other Products]
 Molecular Weight    67,184 Da
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 NCBI Official Full Name    galactocerebrosidase isoform c
 NCBI Official Synonym Full Names    galactosylceramidase
 NCBI Official Symbol    GALC [Similar Products]
 NCBI Protein Information    galactocerebrosidase
 UniProt Protein Name    Galactocerebrosidase
 UniProt Synonym Protein Names   
Galactocerebroside beta-galactosidase; Galactosylceramidase; Galactosylceramide beta-galactosidase
 Protein Family    Galactocerebrosidase
 UniProt Gene Name    GALC [Similar Products]
 UniProt Synonym Gene Names    GALCERase [Similar Products]
 UniProt Entry Name    GALC_HUMAN
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 NCBI Summary for GALC    This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GALC    GALC: Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. Defects in GALC are the cause of leukodystrophy globoid cell (GLD); also known as Krabbe disease. This autosomal recessive disorder results in the insufficient catabolism of several galactolipids that are important in the production of normal myelin. Clinically, the most frequent form is the infantile form. Most patients (90%) present before six months of age with irritability, spasticity, arrest of motor and mental development, and bouts of temperature elevation without infection. This is followed by myoclonic jerks of arms and legs, oposthotonus, hypertonic fits, and mental regression, which progresses to a severe decerebrate condition with no voluntary movements and death from respiratory infections or cerebral hyperpyrexia before 2 years of age. However, a significant number of cases with later onset, presenting with unexplained blindness, weakness and/or progressive motor, and sensory neuropathy that can progress to severe mental incapacity and death, have been identified. Belongs to the glycosyl hydrolase 59 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; Hydrolase; EC 3.2.1.46

Chromosomal Location of Human Ortholog: 14q31

Cellular Component: lysosomal lumen; lysosome

Molecular Function: galactosylceramidase activity

Biological Process: carbohydrate metabolic process; galactosylceramide catabolic process; glycosphingolipid metabolic process; sphingolipid metabolic process

Disease: Krabbe Disease
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 Research Articles on GALC    1. Genetic variants near IREB2 and GALC likely contribute to genetic susceptibility to PAE associated with COPD.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GALC elisa kitPathways associated with GALC elisa kit
 Reference Product  PubMed Publications
 SMPD1 elisa kit  >11 publications with GALC and SMPD1
 UGCG elisa kit  >3 publications with GALC and UGCG
 ARSA elisa kit  >3 publications with GALC and ARSA
 GAL3ST1 elisa kit  >2 publications with GALC and GAL3ST1
 ASAH1 elisa kit  >2 publications with GALC and ASAH1
 SGPP1 elisa kit  >1 publications with GALC and SGPP1
 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
Diseases associated with GALC elisa kitOrgans/Tissues associated with GALC elisa kit
 Disease Name  Pubmed Publications
 Leukodystrophy, Globoid Cell Antibodies  >953 publications with GALC and Leukodystrophy, Globoid Cell
 Disease Models, Animal Antibodies  >161 publications with GALC and Disease Models, Animal
 Atrophy Antibodies  >51 publications with GALC and Atrophy
 Congenital Abnormalities Antibodies  >38 publications with GALC and Congenital Abnormalities
 Seizures Antibodies  >27 publications with GALC and Seizures
 Nervous System Malformations Antibodies  >25 publications with GALC and Nervous System Malformations
 Inflammation Antibodies  >24 publications with GALC and Inflammation
 Necrosis Antibodies  >23 publications with GALC and Necrosis
 Liver Diseases Antibodies  >21 publications with GALC and Liver Diseases
 Kidney Diseases Antibodies  >13 publications with GALC and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >610 publications with GALC and Brain
 Blood Antibodies  >187 publications with GALC and Blood
 Bone Antibodies  >97 publications with GALC and Bone
 Bone Marrow Antibodies  >88 publications with GALC and Bone Marrow
 Skin Antibodies  >67 publications with GALC and Skin
 Liver Antibodies  >61 publications with GALC and Liver
 Embryonic Tissue Antibodies  >46 publications with GALC and Embryonic Tissue
 Kidney Antibodies  >41 publications with GALC and Kidney
 Muscle Antibodies  >38 publications with GALC and Muscle
 Amniotic Fluid Antibodies  >27 publications with GALC and Amniotic Fluid
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