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Gal-7 elisa kit :: Human Galectin 7 (Gal-7) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701810 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
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 Product Name   

Galectin 7 (Gal-7), ELISA Kit

★Popular Item★
 Also Known As   

Human Galectin 7, Gal-7 ELISA Kit

 Product Synonym Names    Human Galectin 7 (Gal-7) ELISA Kit; Galectin 7 (Gal-7)
 Product Gene Name   

Gal-7 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701810 Testing Data    Testing Data PDF
 OMIM    600615
 3D Structure    ModBase 3D Structure for P47929
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human Gal-7. No significant cross-reactivity or interference between Human Gal-7 and analogues was observed.
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 Detection Range    0.312 ng/ml-20 ng/ml
 Sensitivity    0.078 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Gal-7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Gal-7 purchase    MBS701810 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Galectin 7 (Gal-7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing Gal-7, with an estimated sensitivity of 0.078 ng/ml. The ELISA analytical biochemical technique of the MBS701810 kit is based on Gal-7 antibody-Gal-7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Gal-7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Gal-7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Gal-7 elisa kit

   Introduction: Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Galectin 7 is thought to be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Galectin 7 has been implicated in apoptosis and is believed to upregulate MMP9 and as such be involved in the progression of tumorigenesis. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gal-7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Gal-7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Gal-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Gal-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Gal-7 elisa kit    Gal-7 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701810. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Gal-7. It may not necessarily be applicable to this product.
 NCBI GI #    109948279
 NCBI GeneID    653499
 NCBI Accession #    NP_001035972.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001042507.3 [Other Products]
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 UniProt Primary Accession #    P47929 [Other Products]
 UniProt Secondary Accession #    Q6IB87 [Other Products]
 UniProt Related Accession #    P47929 [Other Products]
 Molecular Weight    15,075 Da
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 NCBI Official Full Name    galectin-7
 NCBI Official Synonym Full Names    lectin, galactoside-binding, soluble, 7B
 NCBI Official Symbol    LGALS7B [Similar Products]
 NCBI Official Synonym Symbols   
PI7; GAL7; Gal-7; HKL-14; LGALS7
[Similar Products]
 NCBI Protein Information    galectin-7; galectin 7B; galectin-7B; p53-induced protein 1; keratinocyte lectin 14; p53-induced gene 1 protein; lectin galactoside-binding soluble 7
 UniProt Protein Name    Galectin-7
 UniProt Synonym Protein Names   
HKL-14; PI7; p53-induced gene 1 protein
 UniProt Gene Name    LGALS7 [Similar Products]
 UniProt Synonym Gene Names    PIG1
LGALS7B; Gal-7
[Similar Products]
 UniProt Entry Name    LEG7_HUMAN
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 NCBI Summary for Gal-7    The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:3963) is found adjacent to, but on the opposite strand on chromosome 19. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Gal-7    Function: Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.1 PublicationManual assertion based on experiment in:Ref.6
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 Research Articles on Gal-7    1. An association was seen between the level of presence of galectins-1 and -7 and neoplastic progression of hypopharyngeal and laryngeal squamous cell carcinomas.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with Gal-7 elisa kitOrgans/Tissues associated with Gal-7 elisa kit
 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >1 publications with Gal-7 and Skin Diseases
 Precancerous Conditions Antibodies  >1 publications with Gal-7 and Precancerous Conditions
 Inflammation Antibodies  >1 publications with Gal-7 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >10 publications with Gal-7 and Skin
 Larynx Antibodies  >2 publications with Gal-7 and Larynx
 Eye Antibodies  >2 publications with Gal-7 and Eye
 Brain Antibodies  >1 publications with Gal-7 and Brain
 Mouth Antibodies  >1 publications with Gal-7 and Mouth
 Heart Antibodies  >1 publications with Gal-7 and Heart
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