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GasR elisa kit :: Human GasR ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2503460
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GasR, ELISA Kit

★Popular Item★
 Also Known As   

Human GasR (Gastrin Receptor) ELISA Kit

 Product Gene Name   

GasR elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    118445
 3D Structure    ModBase 3D Structure for P32239
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human GasR. No significant cross-reactivity or interference between Human GasR and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Min: 0.094ng/mL; Max: 10ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GasR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GasR purchase    MBS2503460 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GasR, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing GasR, with an estimated sensitivity of Min: 0.094ng/mL; Max: 10ng/mL. The ELISA analytical biochemical technique of the MBS2503460 kit is based on GasR antibody-GasR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GasR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GasR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GasR elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GasR concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GasR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GasR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GasR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GasR. You can calculate the concentration of GasR in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of GasR elisa kit    GasR elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2503460. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GasR. It may not necessarily be applicable to this product.
 NCBI GI #    28875799
 NCBI GeneID    887
 NCBI Accession #    NP_795344.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_176875.3 [Other Products]
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 UniProt Primary Accession #    P32239 [Other Products]
 UniProt Secondary Accession #    O75824; Q16144; Q92492; Q96LC6; Q9NYK7; Q9UBV1; A8K7P9 [Other Products]
 UniProt Related Accession #    P32239 [Other Products]
 Molecular Weight    41,504 Da
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 NCBI Official Full Name    gastrin/cholecystokinin type B receptor
 NCBI Official Synonym Full Names    cholecystokinin B receptor
 NCBI Official Symbol    CCKBR [Similar Products]
 NCBI Official Synonym Symbols   
GASR; CCK-B; CCK2R
[Similar Products]
 NCBI Protein Information    gastrin/cholecystokinin type B receptor; CCK-B receptor; CCK-BR; CCK2 receptor; CCK2-R; cholecystokinin-2 receptor; gastrin receptor
 UniProt Protein Name    Gastrin/cholecystokinin type B receptor
 UniProt Synonym Protein Names   
Cholecystokinin-2 receptor; CCK2-R
 UniProt Gene Name    CCKBR [Similar Products]
 UniProt Synonym Gene Names    CCKRB; CCK-B receptor; CCK-BR; CCK2-R [Similar Products]
 UniProt Entry Name    GASR_HUMAN
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 NCBI Summary for GasR    This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GasR    CCKBR: Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: type B gastrin/cholecystokinin receptor binding; protein binding; gastrin receptor activity; 1-phosphatidylinositol-3-kinase regulator activity; cholecystokinin receptor activity; phosphoinositide phospholipase C activity

Biological Process: gland development; gastric acid secretion; cell proliferation; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; gut development; metabolic process; sensory perception; digestion; positive regulation of cell proliferation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); feeding behavior; regulation of phosphoinositide 3-kinase activity
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 Research Articles on GasR    1. Our results indicate a promoting role of CCK2R on GIST tumourigenesis, particularly in tumours of gastric origin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GasR elisa kitPathways associated with GasR elisa kit
 Reference Product  PubMed Publications
 CCK elisa kit  >696 publications with GasR and CCK
 CCKAR elisa kit  >15 publications with GasR and CCKAR
 NTS elisa kit  >6 publications with GasR and NTS
 GRP elisa kit  >4 publications with GasR and GRP
 TAC1 elisa kit  >1 publications with GasR and TAC1
 GCG elisa kit  >1 publications with GasR and GCG
 GAST elisa kit  >1 publications with GasR and GAST
 Products by Pathway  Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 GPCRs, Other Pathway antibodies  GPCRs, Other Pathway Diagram
Diseases associated with GasR elisa kitOrgans/Tissues associated with GasR elisa kit
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >73 publications with GasR and Mental Disorders
 Nervous System Diseases Antibodies  >55 publications with GasR and Nervous System Diseases
 Disease Models, Animal Antibodies  >48 publications with GasR and Disease Models, Animal
 Panic Disorder Antibodies  >38 publications with GasR and Panic Disorder
 Liver Neoplasms Antibodies  >18 publications with GasR and Liver Neoplasms
 Hyperplasia Antibodies  >15 publications with GasR and Hyperplasia
 Hyperalgesia Antibodies  >14 publications with GasR and Hyperalgesia
 Atrophy Antibodies  >12 publications with GasR and Atrophy
 Learning Disorders Antibodies  >9 publications with GasR and Learning Disorders
 Necrosis Antibodies  >9 publications with GasR and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >257 publications with GasR and Brain
 Stomach Antibodies  >183 publications with GasR and Stomach
 Lung Antibodies  >22 publications with GasR and Lung
 Embryonic Tissue Antibodies  >7 publications with GasR and Embryonic Tissue
 Eye Antibodies  >4 publications with GasR and Eye
 Placenta Antibodies  >1 publications with GasR and Placenta
 Testis Antibodies  >1 publications with GasR and Testis
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