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FABP6 elisa kit :: Human Gastrotropin (FABP6) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7233299
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Gastrotropin (FABP6), ELISA Kit

★Popular Item★
 Also Known As   

Human Gastrotropin (FABP6) ELISA Kit

 Product Gene Name   

FABP6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600422
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of FABP6. No significant cross-reactivity or interference between FABP6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FABP6 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    250-5000 pg/mL
 Sensitivity    1.0PG/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FABP6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FABP6 purchase    MBS7233299 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gastrotropin (FABP6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 250-5000 pg/mL in biological research samples containing FABP6, with an estimated sensitivity of 1.0PG/mL. The ELISA analytical biochemical technique of the MBS7233299 kit is based on FABP6 antibody-FABP6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FABP6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FABP6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for FABP6 elisa kit

   Intended Uses: This FABP6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FABP6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: FABP6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FABP6 antibody and an FABP6-HRP conjugate. The assay sample and buffer are incubated together with FABP6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FABP6 concentration since FABP6 from samples and FABP6-HRP conjugate compete for the anti-FABP6 antibody binding site. Since the number of sites is limited, as more sites are occupied by FABP6 from the sample, fewer sites are left to bind FABP6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP6 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve of FABP6 elisa kit    FABP6 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7233299. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FABP6. It may not necessarily be applicable to this product.
 NCBI GI #    94721243
 NCBI GeneID    2172
 NCBI Accession #    NP_001035532.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001040442.1 [Other Products]
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 UniProt Secondary Accession #    Q07DR7; Q8TBI3; Q9UGI7 [Other Products]
 UniProt Related Accession #    P51161 [Other Products]
 Molecular Weight    19,874 Da
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 NCBI Official Full Name    gastrotropin isoform 1
 NCBI Official Synonym Full Names    fatty acid binding protein 6, ileal
 NCBI Official Symbol    FABP6 [Similar Products]
 NCBI Official Synonym Symbols   
ILBP; I-15P; I-BAP; ILBP3; ILLBP; I-BABP; I-BALB
[Similar Products]
 NCBI Protein Information    gastrotropin; GT; intestinal 15 kDa protein; ileal lipid-binding protein; illeal lipid-binding protein; ileal bile acid binding protein
 UniProt Protein Name    Gastrotropin
 UniProt Synonym Protein Names   
Fatty acid-binding protein 6; Ileal lipid-binding protein; ILBP; Intestinal 15 kDa protein; I-15P; Intestinal bile acid-binding protein; I-BABP
 Protein Family    Gastrotropin
 UniProt Gene Name    FABP6 [Similar Products]
 UniProt Synonym Gene Names    ILBP; ILLBP; GT; ILBP; I-15P; I-BABP [Similar Products]
 UniProt Entry Name    FABP6_HUMAN
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 NCBI Summary for FABP6    This gene encodes the ileal fatty acid binding protein. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP6 and FABP1 (the liver fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Transcript variants generated by alternate transcription promoters and/or alternate splicing have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FABP6    FABP6: Ileal protein which stimulates gastric acid and pepsinogen secretion. Seems to be able to bind to bile salts and bilirubins. Isoform 2 is essential for the survival of colon cancer cells to bile acid-induced apoptosis. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 5q33.3-q34

Cellular Component: cytoplasm; cytosol

Molecular Function: transporter activity; lipid binding

Biological Process: bile acid and bile salt transport; negative regulation of cell proliferation; bile acid metabolic process; lipid metabolic process; transmembrane transport
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 Research Articles on FABP6    1. show, using electrospray ionization mass spectroscopy, that human ILBP binds bile acids with a 3:1 ratio, even at low protein and ligand concentrations
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FABP6 elisa kitPathways associated with FABP6 elisa kit
 Reference Product  PubMed Publications
 ABCC3 elisa kit  >1 publications with FABP6 and ABCC3
 SLC10A2 elisa kit  >1 publications with FABP6 and SLC10A2
 Products by Pathway  Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 Recycling Of Bile Acids And Salts Pathway antibodies  Recycling Of Bile Acids And Salts Pathway Diagram
Diseases associated with FABP6 elisa kitOrgans/Tissues associated with FABP6 elisa kit
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >9 publications with FABP6 and Fatty Liver
 Breast Neoplasms Antibodies  >1 publications with FABP6 and Breast Neoplasms
 Liver Neoplasms Antibodies  >1 publications with FABP6 and Liver Neoplasms
 Atherosclerosis Antibodies  >1 publications with FABP6 and Atherosclerosis
 Colonic Neoplasms Antibodies  >1 publications with FABP6 and Colonic Neoplasms
 Disease Models, Animal Antibodies  >1 publications with FABP6 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >1 publications with FABP6 and Neoplasms, Experimental
 Liver Neoplasms, Experimental Antibodies  >1 publications with FABP6 and Liver Neoplasms, Experimental
 Hyperplasia Antibodies  >1 publications with FABP6 and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with FABP6 and Brain
 Ovary Antibodies  >2 publications with FABP6 and Ovary
 Kidney Antibodies  >2 publications with FABP6 and Kidney
 Testis Antibodies  >1 publications with FABP6 and Testis
 Lymph Antibodies  >1 publications with FABP6 and Lymph
 Eye Antibodies  >1 publications with FABP6 and Eye
 Connective Tissue Antibodies  >1 publications with FABP6 and Connective Tissue
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