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GHRL elisa kit :: Human Ghrelin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS283919 GHRL elisa kit
Unit / Price
48-Strip-Wells  /  $465 +1 FREE 8GB USB
96-Strip-Wells  /  $790 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,500 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,655 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ghrelin (GHRL), ELISA Kit

★Popular Item★
 Also Known As   

Human Ghrelin (GHRL) ELISA Kit

 Product Synonym Names    MTLRP; obestatin; OTTHUMP00000207794|OTTHUMP00000207795|OTTHUMP00000207796|OTTHUMP00000207798|ghrelin; growth hormone secretagogue receptor ligand|ghrelin/obestatin|ghrelin/obestatin preprohormone|m
 Product Gene Name   

GHRL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    AB029434 mRNA
 3D Structure    ModBase 3D Structure for Q9UBU3
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human GHRL. No significant cross-reactivity or interference between Human GHRL and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GHRL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GHRL purchase    MBS283919 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ghrelin (GHRL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GHRL. The ELISA analytical biochemical technique of the MBS283919 kit is based on GHRL antibody-GHRL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GHRL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GHRL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GHRL elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GHRL in samples. An antibody specific for GHRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGHRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GHRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHRL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS283919. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GHRL. It may not necessarily be applicable to this product.
 NCBI GI #    201860279
 NCBI GeneID    51738
 NCBI Accession #    NP_001128413.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001134941.2 [Other Products]
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 UniProt Primary Accession #    Q9UBU3 [Other Products]
 UniProt Secondary Accession #    Q86YP8; Q8TAT9; Q9H3R3; A8CF34; A8CF38; A8CF42; A8DN29; A8DN30 [Other Products]
 UniProt Related Accession #    Q9UBU3 [Other Products]
 Molecular Weight    9,972 Da
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 NCBI Official Full Name    appetite-regulating hormone isoform 2 preproprotein
 NCBI Official Synonym Full Names    ghrelin and obestatin prepropeptide
 NCBI Official Symbol    GHRL [Similar Products]
 NCBI Official Synonym Symbols   
MTLRP
[Similar Products]
 NCBI Protein Information    appetite-regulating hormone
 UniProt Protein Name    Appetite-regulating hormone
 UniProt Synonym Protein Names   
Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46
 Protein Family    Ghrelin
 UniProt Gene Name    GHRL [Similar Products]
 UniProt Synonym Gene Names    MTLRP; Ghrelin [Similar Products]
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 NCBI Summary for GHRL    This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]
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 UniProt Comments for GHRL    ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach

Protein type: Apoptosis; Cell cycle regulation; Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: axon; endoplasmic reticulum lumen; extracellular region; extracellular space

Molecular Function: G-protein coupled receptor binding; ghrelin receptor binding; growth hormone-releasing hormone activity; protein binding; protein tyrosine kinase activator activity

Biological Process: actin polymerization or depolymerization; activation of MAPK activity; adult feeding behavior; cartilage development; cortisol secretion; decidualization; dendrite development; elevation of cytosolic calcium ion concentration; excitatory postsynaptic potential; G-protein coupled receptor signaling pathway; gastric acid secretion; glucose metabolic process; growth hormone secretion; hormone-mediated signaling pathway; negative regulation of angiogenesis; negative regulation of apoptosis; negative regulation of circadian sleep/wake cycle, REM sleep; negative regulation of endothelial cell proliferation; negative regulation of inflammatory response; negative regulation of insulin secretion; negative regulation of interleukin-1 beta production; negative regulation of interleukin-6 biosynthetic process; negative regulation of locomotion; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of appetite; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of corticotropin secretion; positive regulation of cortisol secretion; positive regulation of growth hormone secretion; positive regulation of growth rate; positive regulation of insulin secretion; positive regulation of multicellular organism growth; positive regulation of synaptogenesis; regulation of cell proliferation; regulation of response to food; regulation of transmission of nerve impulse; response to electrical stimulus; response to estrogen; response to hormone

Disease: Obesity
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 Research Articles on GHRL    1. Data suggest that subjects with anorexia nervosa display broad spectrum of physical activity (2479-26,047 steps/day) which shows a negative correlation with plasma kisspeptin levels and a positive association with plasma ghrelin levels.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GHRL elisa kitPathways associated with GHRL elisa kit
 Reference Product  PubMed Publications
 GHSR elisa kit  >28 publications with GHRL and GHSR
 NPY elisa kit  >12 publications with GHRL and NPY
 LEP elisa kit  >11 publications with GHRL and LEP
 CCK elisa kit  >5 publications with GHRL and CCK
 GHRH elisa kit  >3 publications with GHRL and GHRH
 AGRP elisa kit  >3 publications with GHRL and AGRP
 GCG elisa kit  >2 publications with GHRL and GCG
 IGF1 elisa kit  >1 publications with GHRL and IGF1
 GPR39 elisa kit  >1 publications with GHRL and GPR39
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Metabolic Disorders Of Biological Oxidation Enzymes Pathway antibodies  Metabolic Disorders Of Biological Oxidation Enzymes Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
Diseases associated with GHRL elisa kitOrgans/Tissues associated with GHRL elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >53 publications with GHRL and Obesity
 Insulin Resistance Antibodies  >18 publications with GHRL and Insulin Resistance
 Nervous System Diseases Antibodies  >13 publications with GHRL and Nervous System Diseases
 Weight Gain Antibodies  >12 publications with GHRL and Weight Gain
 Inflammation Antibodies  >10 publications with GHRL and Inflammation
 Cachexia Antibodies  >7 publications with GHRL and Cachexia
 Kidney Diseases Antibodies  >5 publications with GHRL and Kidney Diseases
 Heart Diseases Antibodies  >5 publications with GHRL and Heart Diseases
 Breast Neoplasms Antibodies  >5 publications with GHRL and Breast Neoplasms
 Necrosis Antibodies  >5 publications with GHRL and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >80 publications with GHRL and Blood
 Stomach Antibodies  >27 publications with GHRL and Stomach
 Brain Antibodies  >23 publications with GHRL and Brain
 Connective Tissue Antibodies  >11 publications with GHRL and Connective Tissue
 Intestine Antibodies  >4 publications with GHRL and Intestine
 Prostate Antibodies  >2 publications with GHRL and Prostate
 Embryonic Tissue Antibodies  >1 publications with GHRL and Embryonic Tissue
 Lung Antibodies  >1 publications with GHRL and Lung
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