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GFAP elisa kit :: Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023807 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $255 +1 FREE 8GB USB
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $610 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,470 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glial Fibrillary Acidic Protein (GFAP), ELISA Kit

★Popular Item★
 Also Known As   

Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

 Product Synonym Names    Intermediate Filament Protein
 Product Gene Name   

GFAP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    137780
 3D Structure    ModBase 3D Structure for P14136
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Glial Fibrillary Acidic Protein (GFAP).
No significant cross-reactivity or interference between Glial Fibrillary Acidic Protein (GFAP) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.056ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Glial Fibrillary Acidic Protein (GFAP) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Glial Fibrillary Acidic Protein (GFAP) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GFAP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GFAP purchase    MBS2023807 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glial Fibrillary Acidic Protein (GFAP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing GFAP, with an estimated sensitivity of < 0.056ng/mL. The ELISA analytical biochemical technique of the MBS2023807 kit is based on GFAP antibody-GFAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GFAP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GFAP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GFAP elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glial Fibrillary Acidic Protein (GFAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glial Fibrillary Acidic Protein (GFAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glial Fibrillary Acidic Protein (GFAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glial Fibrillary Acidic Protein (GFAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for GFAP elisa kit    Infection immunity; Neuroscience
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 Typical Testing Data/Standard Curve (for reference only) of GFAP elisa kit    GFAP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023807. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GFAP. It may not necessarily be applicable to this product.
 NCBI GI #    251802
 NCBI GeneID    2670
 NCBI Accession #    AAB22581.1 [Other Products]
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 UniProt Primary Accession #    P14136 [Other Products]
 UniProt Secondary Accession #    Q53H98; Q5D055; Q6ZQS3; Q7Z5J6; Q7Z5J7; Q96KS4; Q96P18; Q9UFD0; B2RD44; D3DX59; E9PAX3 [Other Products]
 UniProt Related Accession #    P14136 [Other Products]
 Molecular Weight    49,508 Da
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 NCBI Official Full Name    glial fibrillary acidic protein
 NCBI Official Synonym Full Names    glial fibrillary acidic protein
 NCBI Official Symbol    GFAP [Similar Products]
 NCBI Official Synonym Symbols   
ALXDRD
[Similar Products]
 NCBI Protein Information    glial fibrillary acidic protein
 UniProt Protein Name    Glial fibrillary acidic protein
 Protein Family    Glial fibrillary acidic protein
 UniProt Gene Name    GFAP [Similar Products]
 UniProt Synonym Gene Names    GFAP [Similar Products]
 UniProt Entry Name    GFAP_HUMAN
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 NCBI Summary for GFAP    This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
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 UniProt Comments for GFAP    GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: membrane; cytoplasm; intermediate filament; cytosol

Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding

Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; intermediate filament organization; neurite regeneration; astrocyte development

Disease: Alexander Disease
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 Research Articles on GFAP    1. Identification of a novel nonsense mutation in the rod domain of GFAP that is associated with Alexander disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GFAP elisa kitDiseases associated with GFAP elisa kit
 Products by Pathway  Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Nuclear Signaling By ERBB4 Pathway antibodies  Nuclear Signaling By ERBB4 Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By ERBB4 Pathway antibodies  Signaling By ERBB4 Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4887 publications with GFAP and Nervous System Diseases
 Brain Diseases Antibodies  >3690 publications with GFAP and Brain Diseases
 Disease Models, Animal Antibodies  >1426 publications with GFAP and Disease Models, Animal
 Gliosis Antibodies  >1104 publications with GFAP and Gliosis
 Cardiovascular Diseases Antibodies  >873 publications with GFAP and Cardiovascular Diseases
 Brain Injuries Antibodies  >696 publications with GFAP and Brain Injuries
 Inflammation Antibodies  >629 publications with GFAP and Inflammation
 Necrosis Antibodies  >565 publications with GFAP and Necrosis
 Nerve Degeneration Antibodies  >540 publications with GFAP and Nerve Degeneration
 Epilepsy Antibodies  >365 publications with GFAP and Epilepsy
Organs/Tissues associated with GFAP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7853 publications with GFAP and Brain
 Nerve Antibodies  >3388 publications with GFAP and Nerve
 Blood Antibodies  >1583 publications with GFAP and Blood
 Spinal Cord Antibodies  >1384 publications with GFAP and Spinal Cord
 Eye Antibodies  >993 publications with GFAP and Eye
 Muscle Antibodies  >516 publications with GFAP and Muscle
 Ganglia Antibodies  >295 publications with GFAP and Ganglia
 Bone Marrow Antibodies  >252 publications with GFAP and Bone Marrow
 Liver Antibodies  >207 publications with GFAP and Liver
 Connective Tissue Antibodies  >186 publications with GFAP and Connective Tissue
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