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G6PC elisa kit :: Human Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS454366
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $400 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,425 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,710 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glucose-6-Phosphatase, Catalytic (G6PC), ELISA Kit

★Popular Item★
 Also Known As   

Human Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit

 Product Gene Name   

G6PC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    U01120 mRNA
 3D Structure    ModBase 3D Structure for P35575
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of G6PC. No significant cross-reactivity or interference between G6PC and analogues was observed.
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 Samples    Tissue homogenates, cell lysates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.063ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G6PC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G6PC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of G6PC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for G6PC purchase    MBS454366 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing G6PC, with an estimated sensitivity of 0.063ng/mL. The ELISA analytical biochemical technique of the MBS454366 kit is based on G6PC antibody-G6PC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect G6PC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, G6PC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for G6PC elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of G6PC in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to G6PC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to G6PC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G6PC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of G6PC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of G6PC elisa kit    G6PC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS454366. Click to request current manual
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NCBI/Uniprot data below describe general gene information for G6PC. It may not necessarily be applicable to this product.
 NCBI GI #    393537031
 NCBI GeneID    2538
 NCBI Accession #    NP_000142.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000151.3 [Other Products]
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 UniProt Primary Accession #    P35575 [Other Products]
 UniProt Secondary Accession #    A1L4C0; B4E1C3; K7EL82 [Other Products]
 UniProt Related Accession #    P35575 [Other Products]
 Molecular Weight    20,209 Da
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 NCBI Official Full Name    glucose-6-phosphatase isoform 1
 NCBI Official Synonym Full Names    glucose-6-phosphatase catalytic subunit
 NCBI Official Symbol    G6PC [Similar Products]
 NCBI Official Synonym Symbols   
G6PT; GSD1; G6PC1; GSD1a; G6Pase
[Similar Products]
 NCBI Protein Information    glucose-6-phosphatase
 UniProt Protein Name    Glucose-6-phosphatase
 UniProt Synonym Protein Names   
Glucose-6-phosphatase alpha; G6Pase-alpha
 Protein Family    Glucose-6-phosphatase
 UniProt Gene Name    G6PC [Similar Products]
 UniProt Synonym Gene Names    G6PT; G-6-Pase; G6Pase; G6Pase-alpha [Similar Products]
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 NCBI Summary for G6PC    Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]
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 UniProt Comments for G6PC    Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.
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 Research Articles on G6PC    1. Post-translational regulation of the glucose-6-phosphatase complex by cyclic AMP is a crucial determinant of endogenous glucose production and is controlled by the glucose-6-phosphate transporter.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with G6PC elisa kitPathways associated with G6PC elisa kit
 Reference Product  PubMed Publications
 FOXO1 elisa kit  >15 publications with G6PC and FOXO1
 HNF1A elisa kit  >3 publications with G6PC and HNF1A
 CREB1 elisa kit  >1 publications with G6PC and CREB1
 PGM1 elisa kit  >1 publications with G6PC and PGM1
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Angiopoietin Like Protein 8 Regulatory Pathway antibodies  Angiopoietin Like Protein 8 Regulatory Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 FoxO Family Signaling Pathway antibodies  FoxO Family Signaling Pathway Diagram
Diseases associated with G6PC elisa kitOrgans/Tissues associated with G6PC elisa kit
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >46 publications with G6PC and Insulin Resistance
 Hepatorenal form of glycogen storage disease Antibodies  >41 publications with G6PC and Hepatorenal form of glycogen storage disease
 Glycogen Storage Disease Type I Antibodies  >41 publications with G6PC and Glycogen Storage Disease Type I
 Liver Diseases Antibodies  >39 publications with G6PC and Liver Diseases
 Fatty Liver Antibodies  >32 publications with G6PC and Fatty Liver
 Disease Models, Animal Antibodies  >25 publications with G6PC and Disease Models, Animal
 Hyperglycemia Antibodies  >23 publications with G6PC and Hyperglycemia
 Hepatomegaly Antibodies  >15 publications with G6PC and Hepatomegaly
 Inflammation Antibodies  >11 publications with G6PC and Inflammation
 Carcinoma, Hepatocellular Antibodies  >11 publications with G6PC and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >129 publications with G6PC and Liver
 Kidney Antibodies  >24 publications with G6PC and Kidney
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