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GRIN2B elisa kit :: Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020683 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $380 +1 FREE 8GB USB
48-Strip-Wells  /  $565 +1 FREE 8GB USB
96-Strip-Wells  /  $765 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,130 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,670 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B), ELISA Kit

 Also Known As   

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) ELISA Kit

 Product Synonym Names    NMDAR2B; NR2B; hNR3; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3
 Product Gene Name   

GRIN2B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    138252
 3D Structure    ModBase 3D Structure for Q13224
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B).
No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) and analogues was observed.
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 Samples    Tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    Typically less than 0.147ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GRIN2B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GRIN2B purchase    MBS2020683 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing GRIN2B, with an estimated sensitivity of Typically less than 0.147ng/mL. The ELISA analytical biochemical technique of the MBS2020683 kit is based on GRIN2B antibody-GRIN2B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GRIN2B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GRIN2B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for GRIN2B elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of GRIN2B elisa kit    GRIN2B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020683. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GRIN2B. It may not necessarily be applicable to this product.
 NCBI GI #    167003331
 NCBI GeneID    2904
 NCBI Accession #    NP_000825.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000834.3 [Other Products]
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 UniProt Primary Accession #    Q13224 [Other Products]
 UniProt Secondary Accession #    Q12919; Q13220; Q13225; Q14CU4; Q9UM56 [Other Products]
 UniProt Related Accession #    Q13224 [Other Products]
 Molecular Weight    166,367 Da
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 NCBI Official Full Name    glutamate receptor ionotropic, NMDA 2B
 NCBI Official Synonym Full Names    glutamate receptor, ionotropic, N-methyl D-aspartate 2B
 NCBI Official Symbol    GRIN2B [Similar Products]
 NCBI Official Synonym Symbols   
MRD6; NR2B; hNR3; EIEE27; GluN2B; NMDAR2B
[Similar Products]
 NCBI Protein Information    glutamate receptor ionotropic, NMDA 2B
 UniProt Protein Name    Glutamate receptor ionotropic, NMDA 2B
 UniProt Synonym Protein Names   
Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B; N-methyl-D-aspartate receptor subunit 3; NR3; hNR3
 Protein Family    Glutamate receptor ionotropic
 UniProt Gene Name    GRIN2B [Similar Products]
 UniProt Synonym Gene Names    NMDAR2B; GluN2B; NMDAR2B; NR2B; NR3; hNR3 [Similar Products]
 UniProt Entry Name    NMDE2_HUMAN
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 NCBI Summary for GRIN2B    N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GRIN2B    NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

Protein type: Membrane protein, integral; Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12p12

Cellular Component: postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; integral to plasma membrane; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; glycine binding; N-methyl-D-aspartate selective glutamate receptor activity; calcium channel activity

Biological Process: synaptic transmission, glutamatergic; axon guidance; startle response; behavioral fear response; in utero embryonic development; glutamate signaling pathway; regulation of synaptic plasticity; learning; memory; detection of mechanical stimulus involved in sensory perception of pain; synaptic transmission; behavioral response to pain; response to ethanol; learning and/or memory; sensory organ development; suckling behavior; ionotropic glutamate receptor signaling pathway; transport; ephrin receptor signaling pathway; regulation of excitatory postsynaptic membrane potential

Disease: Mental Retardation, Autosomal Dominant 6; Epileptic Encephalopathy, Early Infantile, 27
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 Research Articles on GRIN2B    1. 421C/A polymorphism not associated with risk of occurrence of primary open-angle glaucoma
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GRIN2B elisa kitPathways associated with GRIN2B elisa kit
 Reference Product  PubMed Publications
 GRIN1 elisa kit  >24 publications with GRIN2B and GRIN1
 GRIN3A elisa kit  >3 publications with GRIN2B and GRIN3A
 CAMK2A elisa kit  >1 publications with GRIN2B and CAMK2A
 FYN elisa kit  >1 publications with GRIN2B and FYN
 DLG4 elisa kit  >1 publications with GRIN2B and DLG4
 Products by Pathway  Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Diseases associated with GRIN2B elisa kitOrgans/Tissues associated with GRIN2B elisa kit
 Disease Name  Pubmed Publications
 Schizophrenia Antibodies  >43 publications with GRIN2B and Schizophrenia
 Nervous System Diseases Antibodies  >35 publications with GRIN2B and Nervous System Diseases
 Brain Diseases Antibodies  >22 publications with GRIN2B and Brain Diseases
 Anxiety Disorders Antibodies  >11 publications with GRIN2B and Anxiety Disorders
 Movement Disorders Antibodies  >11 publications with GRIN2B and Movement Disorders
 Cognition Disorders Antibodies  >10 publications with GRIN2B and Cognition Disorders
 Child Development Disorders, Pervasive Antibodies  >9 publications with GRIN2B and Child Development Disorders, Pervasive
 Substance-Related Disorders Antibodies  >9 publications with GRIN2B and Substance-Related Disorders
 Epilepsy Antibodies  >9 publications with GRIN2B and Epilepsy
 Intellectual Disability Antibodies  >8 publications with GRIN2B and Intellectual Disability
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >68 publications with GRIN2B and Brain
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