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GAPDH elisa kit :: Human Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS733201
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glyceraldehyde 3 Phosphate Dehydrogenase (GAPDH), ELISA Kit

 Also Known As   

Human Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit

 Product Gene Name   

GAPDH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
 Highest Standards    10ng/mL
 Concentration of Standards    Concentration of Standards
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GAPDH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GAPDH purchase    MBS733201 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glyceraldehyde 3 Phosphate Dehydrogenase (GAPDH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing GAPDH, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS733201 kit is based on GAPDH antibody-GAPDH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAPDH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAPDH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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 Product Categories/Family for GAPDH elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of GAPDH elisa kit    GAPDH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS733201. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
 NCBI GI #    122065190
 NCBI GeneID    24383
 NCBI Accession #    P04797.3 [Other Products]
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 UniProt Secondary Accession #    P09328; Q5M916; Q9QWU4 [Other Products]
 UniProt Related Accession #    P04797 [Other Products]
 Molecular Weight    35,828 Da
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 NCBI Official Full Name    Glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Synonym Full Names    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Symbol    Gapdh [Similar Products]
 NCBI Official Synonym Symbols   
Gapd; BARS-38
[Similar Products]
 NCBI Protein Information    glyceraldehyde-3-phosphate dehydrogenase; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate
 UniProt Protein Name    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Synonym Protein Names   
38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
 UniProt Gene Name    Gapdh [Similar Products]
 UniProt Synonym Gene Names    Gapd; GAPDH [Similar Products]
 UniProt Entry Name    G3P_RAT
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 NCBI Summary for GAPDH    This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome. [provided by RefSeq, Jan 2014]
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 UniProt Comments for GAPDH    GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma

Protein type: EC 1.2.1.12; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase

Cellular Component: microtubule cytoskeleton; nuclear membrane; mitochondrion; intracellular membrane-bound organelle; membrane; cytoplasm; plasma membrane; lipid particle; ribonucleoprotein complex; nucleus; cytosol; vesicle

Molecular Function: identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding

Biological Process: regulation of translation; neuron apoptosis; glycolysis; protein stabilization; apoptosis; multicellular organismal development; negative regulation of translation; carbohydrate metabolic process; microtubule cytoskeleton organization and biogenesis; gluconeogenesis
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 Research Articles on GAPDH    1. In human and mouse, Gapdh is a moonlighting protein that functions as a glycolytic enzyme and a nuclear protein nitrosylase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GAPDH elisa kitPathways associated with GAPDH elisa kit
 Reference Product  PubMed Publications
 Actb elisa kit  >224 publications with GAPDH and Actb
 Pgk1 elisa kit  >44 publications with GAPDH and Pgk1
 Egfr elisa kit  >15 publications with GAPDH and Egfr
 Tpi1 elisa kit  >7 publications with GAPDH and Tpi1
 Akt1 elisa kit  >3 publications with GAPDH and Akt1
 Il2 elisa kit  >2 publications with GAPDH and Il2
 Fn1 elisa kit  >1 publications with GAPDH and Fn1
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
Diseases associated with GAPDH elisa kitOrgans/Tissues associated with GAPDH elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >270 publications with GAPDH and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >240 publications with GAPDH and Cardiovascular Diseases
 Necrosis Antibodies  >199 publications with GAPDH and Necrosis
 Brain Diseases Antibodies  >199 publications with GAPDH and Brain Diseases
 Disease Models, Animal Antibodies  >189 publications with GAPDH and Disease Models, Animal
 Inflammation Antibodies  >179 publications with GAPDH and Inflammation
 Heart Diseases Antibodies  >144 publications with GAPDH and Heart Diseases
 Liver Diseases Antibodies  >133 publications with GAPDH and Liver Diseases
 Kidney Diseases Antibodies  >89 publications with GAPDH and Kidney Diseases
 Liver Neoplasms Antibodies  >75 publications with GAPDH and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >520 publications with GAPDH and Muscle
 Brain Antibodies  >473 publications with GAPDH and Brain
 Liver Antibodies  >381 publications with GAPDH and Liver
 Heart Antibodies  >278 publications with GAPDH and Heart
 Kidney Antibodies  >243 publications with GAPDH and Kidney
 Embryonic Tissue Antibodies  >121 publications with GAPDH and Embryonic Tissue
 Artery Antibodies  >121 publications with GAPDH and Artery
 Eye Antibodies  >86 publications with GAPDH and Eye
 Connective Tissue Antibodies  >83 publications with GAPDH and Connective Tissue
 Colon Antibodies  >67 publications with GAPDH and Colon
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