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GOPC elisa kit :: Human Golgi-associated PDZ and coiled-coil motif-containing protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS282916 GOPC elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC), ELISA Kit

 Also Known As   

Human Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) ELISA Kit

 Product Gene Name   

GOPC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    AF287894 mRNA
 3D Structure    ModBase 3D Structure for Q9HD26
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human GOPC. No significant cross-reactivity or interference between Human GOPC and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GOPC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GOPC purchase    MBS282916 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GOPC. The ELISA analytical biochemical technique of the MBS282916 kit is based on GOPC antibody-GOPC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GOPC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GOPC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GOPC elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GOPC in samples. An antibody specific for GOPC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGOPC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GOPC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GOPC bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for GOPC. It may not necessarily be applicable to this product.
 NCBI GI #    62868213
 NCBI GeneID    57120
 NCBI Accession #    NP_001017408.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001017408.2 [Other Products]
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 UniProt Primary Accession #    Q9HD26 [Other Products]
 UniProt Secondary Accession #    Q59FS4; Q969U8; A6NM30 [Other Products]
 UniProt Related Accession #    Q9HD26 [Other Products]
 Molecular Weight    35,130 Da
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 NCBI Official Full Name    Golgi-associated PDZ and coiled-coil motif-containing protein isoform b
 NCBI Official Synonym Full Names    golgi associated PDZ and coiled-coil motif containing
 NCBI Official Symbol    GOPC [Similar Products]
 NCBI Official Synonym Symbols   
CAL; FIG; PIST; GOPC1; dJ94G16.2
[Similar Products]
 NCBI Protein Information    Golgi-associated PDZ and coiled-coil motif-containing protein
 UniProt Protein Name    Golgi-associated PDZ and coiled-coil motif-containing protein
 UniProt Synonym Protein Names   
CFTR-associated ligand; Fused in glioblastoma; PDZ protein interacting specifically with TC10; PIST
 Protein Family    Golgi-associated PDZ and coiled-coil motif-containing protein
 UniProt Gene Name    GOPC [Similar Products]
 UniProt Synonym Gene Names    CAL; FIG; PIST [Similar Products]
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 NCBI Summary for GOPC    This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
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 UniProt Comments for GOPC    PIST: a ubiquitously expressed peripheral membrane protein that contains a PDZ domain and two coiled-coil regions. Associates with the Golgi apparatus and plasma membrane, regulating intracellular protein trafficking and degradation. May play a role in autophagy, and regulate the intracellular trafficking of the ADRB1 receptor. Interacts with GOLGA3 and STX6. The PDZ domain is known to mediate interactions with CLCN3-iso2, ACCN3, CFTR, SSTR5, FDZ5, ADRB1, FZD8, GRID2, mGluR5, mGluR1. Mutations of PDZ residues S302D, T304E, K348D, K350E abrogates the ability of PIST to interact with the CFTR C terminus. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. An oncogenic fusion protein between PIST (aka FIG) and the receptor tyrosine kinase ROS is found in glioblastoma multiform. Unlike other fusion RTK oncogenes, he mechanism of activation of PIST-ROS does not appear to be dimerization. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. Three alternatively spliced human isoforms have been described.

Protein type: Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: cell junction; cytoplasm; dendrite; Golgi apparatus; Golgi membrane; lysosomal membrane; membrane; plasma membrane; postsynaptic density; postsynaptic membrane; protein complex; trans-Golgi network transport vesicle

Molecular Function: frizzled binding; GTPase regulator activity; ion channel binding; protein binding; protein C-terminus binding; protein homodimerization activity

Biological Process: apical protein localization; cytoplasmic sequestering of CFTR protein; ER to Golgi vesicle-mediated transport; Golgi to plasma membrane transport; negative regulation of anion channel activity; negative regulation of protein localization to cell surface; protein homooligomerization; protein transport; regulation of catalytic activity; spermatid nuclear differentiation

Disease: Spermatogenic Failure 6
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 Research Articles on GOPC    1. PIST, a Golgi-associated PDZ domain-containing protein, interacts with PCDH15 in the plasma membrane.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GOPC elisa kitDiseases associated with GOPC elisa kit
 Reference Product  PubMed Publications
 CFTR elisa kit  >22 publications with GOPC and CFTR
 BECN1 elisa kit  >7 publications with GOPC and BECN1
 SSTR5 elisa kit  >2 publications with GOPC and SSTR5
 RHOQ elisa kit  >2 publications with GOPC and RHOQ
 STX6 elisa kit  >2 publications with GOPC and STX6
 FZD5 elisa kit  >1 publications with GOPC and FZD5
 GOLGA3 elisa kit  >1 publications with GOPC and GOLGA3
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >26 publications with GOPC and Fibrosis
 Death Antibodies  >7 publications with GOPC and Death
 Infertility, Male Antibodies  >7 publications with GOPC and Infertility, Male
 Mental Disorders Antibodies  >5 publications with GOPC and Mental Disorders
 Carcinoma Antibodies  >4 publications with GOPC and Carcinoma
 Hypertension Antibodies  >4 publications with GOPC and Hypertension
 Necrosis Antibodies  >3 publications with GOPC and Necrosis
 Anemia Antibodies  >2 publications with GOPC and Anemia
 Atrophy Antibodies  >2 publications with GOPC and Atrophy
 Brain Diseases Antibodies  >2 publications with GOPC and Brain Diseases
Organs/Tissues associated with GOPC elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with GOPC and Brain
 Lung Antibodies  >6 publications with GOPC and Lung
 Nerve Antibodies  >6 publications with GOPC and Nerve
 Kidney Antibodies  >4 publications with GOPC and Kidney
 Testis Antibodies  >4 publications with GOPC and Testis
 Pancreas Antibodies  >2 publications with GOPC and Pancreas
 Ear Antibodies  >2 publications with GOPC and Ear
 Muscle Antibodies  >1 publications with GOPC and Muscle
 Ovary Antibodies  >1 publications with GOPC and Ovary
 Vascular Antibodies  >1 publications with GOPC and Vascular
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