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GRN elisa kit :: Human Granulin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS732364
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Granulin (GRN), ELISA Kit

★Popular Item★
 Also Known As   

Human Granulin ELISA Kit

 Product Gene Name   

GRN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sandwich ELISA Manual Insert    Sandwich ELISA PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    614706
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of GRN. No significant cross-reactivity or interference between GRN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRN and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive or Sandwich
 Sensitivity    1.0 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GRN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GRN purchase    MBS732364 is a ready-to-use microwell, strip plate Quantitative Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Granulin (GRN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GRN, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS732364 kit is based on GRN antibody-GRN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GRN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GRN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GRN elisa kit

   Intended Uses: This GRN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GRN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: GRN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GRN. Standards or samples are then added to the microtiter plate wells and GRN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GRN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GRN are added to each well to “sandwich” the GRN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GRN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRN concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for GRN elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of GRN elisa kit    GRN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for GRN. It may not necessarily be applicable to this product.
 NCBI GI #    183613
 NCBI GeneID    2896
 NCBI Accession #    AAA58617.1 [Other Products]
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 UniProt Secondary Accession #    P23781; P23782; P23783; P23784; Q53HQ8; Q53Y88; Q540U8; Q9BWE7; Q9H8S1; Q9UCH0; D3DX55 [Other Products]
 UniProt Related Accession #    P28799 [Other Products]
 Molecular Weight    44,132 Da
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 NCBI Official Full Name    granulin
 NCBI Official Synonym Full Names    granulin
 NCBI Official Symbol    GRN [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    granulins; acrogranin; progranulin; proepithelin; granulin-epithelin; PC cell-derived growth factor
 UniProt Protein Name    Granulins
 UniProt Synonym Protein Names   
Proepithelin; PEPI
 Protein Family    Granulin
 UniProt Gene Name    GRN [Similar Products]
 UniProt Synonym Gene Names    PEPI [Similar Products]
 UniProt Entry Name    GRN_HUMAN
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 NCBI Summary for GRN    Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GRN    GRN: Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD); also known as tau- negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. Defects in GRN are the cause of neuronal ceroid lipofuscinosis type 11 (CLN11). A form of neuronal ceroid lipofuscinosis characterized by rapidly progressive visual loss due to retinal dystrophy, seizures, cerebellar ataxia, and cerebellar atrophy. Cognitive decline may also occur. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material. Belongs to the granulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: extracellular space; intracellular membrane-bound organelle; mitochondrion

Molecular Function: protein binding; growth factor activity; cytokine activity

Biological Process: blastocyst hatching; signal transduction; positive regulation of epithelial cell proliferation; embryo implantation

Disease: Frontotemporal Lobar Degeneration With Tdp43 Inclusions, Grn-related; Ceroid Lipofuscinosis, Neuronal, 11
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 Research Articles on GRN    1. Reduced circulating GRN levels might be associated with Parkinson's disease risk by pathogenic factors different from rs5848 and rs646776 polymorphisms.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GRN elisa kitPathways associated with GRN elisa kit
 Reference Product  PubMed Publications
 CHMP2B elisa kit  >28 publications with GRN and CHMP2B
 TARDBP elisa kit  >18 publications with GRN and TARDBP
 SLPI elisa kit  >8 publications with GRN and SLPI
 VEGFA elisa kit  >6 publications with GRN and VEGFA
 CCND1 elisa kit  >3 publications with GRN and CCND1
 MMP12 elisa kit  >1 publications with GRN and MMP12
 CCNT1 elisa kit  >1 publications with GRN and CCNT1
 Products by Pathway  Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
Diseases associated with GRN elisa kitOrgans/Tissues associated with GRN elisa kit
 Disease Name  Pubmed Publications
 Dementia Antibodies  >363 publications with GRN and Dementia
 Nervous System Diseases Antibodies  >356 publications with GRN and Nervous System Diseases
 Brain Diseases Antibodies  >334 publications with GRN and Brain Diseases
 Frontotemporal Lobar Degeneration Antibodies  >300 publications with GRN and Frontotemporal Lobar Degeneration
 Frontotemporal Dementia Antibodies  >268 publications with GRN and Frontotemporal Dementia
 Inflammation Antibodies  >67 publications with GRN and Inflammation
 Nerve Degeneration Antibodies  >36 publications with GRN and Nerve Degeneration
 Necrosis Antibodies  >32 publications with GRN and Necrosis
 Cognition Disorders Antibodies  >22 publications with GRN and Cognition Disorders
 Memory Disorders Antibodies  >22 publications with GRN and Memory Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >256 publications with GRN and Brain
 Blood Antibodies  >148 publications with GRN and Blood
 Nerve Antibodies  >63 publications with GRN and Nerve
 Embryonic Tissue Antibodies  >34 publications with GRN and Embryonic Tissue
 Vascular Antibodies  >32 publications with GRN and Vascular
 Bone Antibodies  >29 publications with GRN and Bone
 Muscle Antibodies  >25 publications with GRN and Muscle
 Liver Antibodies  >25 publications with GRN and Liver
 Heart Antibodies  >23 publications with GRN and Heart
 Lung Antibodies  >17 publications with GRN and Lung
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