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GREM1 elisa kit :: Human Gremlin 1 (GREM1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020142 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Gremlin 1 (GREM1), ELISA Kit

★Popular Item★
 Also Known As   

Gremlin 1 (GREM1) ELISA Kit

 Product Synonym Names    PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1
 Product Gene Name   

GREM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for O70326
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Gremlin 1 (GREM1).
No significant cross-reactivity or interference between Gremlin 1 (GREM1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Urine, Tissue homogenates and Other Biological Fluids
 Detection Range    125-8,000pg/mL
 Sensitivity    < 55pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Gremlin 1 (GREM1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Gremlin 1 (GREM1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GREM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GREM1 purchase    MBS2020142 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gremlin 1 (GREM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 125-8,000pg/mL in biological research samples containing GREM1, with an estimated sensitivity of < 55pg/mL. The ELISA analytical biochemical technique of the MBS2020142 kit is based on GREM1 antibody-GREM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GREM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GREM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Urine, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GREM1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gremlin 1 (GREM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gremlin 1 (GREM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gremlin 1 (GREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gremlin 1 (GREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for GREM1 elisa kit    Signal transduction; Tumor immunity; Developmental science; Bone metabolism
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 Typical Testing Data/Standard Curve (for reference only) of GREM1 elisa kit    GREM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020142. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GREM1. It may not necessarily be applicable to this product.
 NCBI GI #    15929743
 NCBI GeneID    23892
 NCBI Accession #    AAH15293.1 [Other Products]
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 UniProt Primary Accession #    O70326 [Other Products]
 UniProt Related Accession #    O70326 [Other Products]
 Molecular Weight    20,710 Da
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 NCBI Official Full Name    Gremlin 1
 NCBI Official Synonym Full Names    gremlin 1
 NCBI Official Symbol    Grem1 [Similar Products]
 NCBI Official Synonym Symbols   
ld; Drm; Grem; Cktsf1b1
[Similar Products]
 NCBI Protein Information    gremlin-1
 UniProt Protein Name    Gremlin-1
 UniProt Synonym Protein Names   
Cysteine knot superfamily 1, BMP antagonist 1; Down-regulated in Mos-transformed cells protein
 Protein Family    Gremlin
 UniProt Gene Name    Grem1 [Similar Products]
 UniProt Synonym Gene Names    Cktsf1b1; Drm [Similar Products]
 UniProt Entry Name    GREM1_MOUSE
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 UniProt Comments for GREM1    GREM1: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. Interacts with SLIT1 and SLIT2 in a glycosylation- dependent manner. By high glucose through TGFB1-mediated pathways in mesangial cell. Down-regulated in tumor cell lines. Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: heparan sulfate proteoglycan binding; transmembrane receptor protein tyrosine kinase activator activity; vascular endothelial growth factor receptor 2 binding; cytokine activity; receptor agonist activity

Biological Process: limb development; collagen fibril organization; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; positive regulation of receptor internalization; cell morphogenesis; positive regulation of NF-kappaB import into nucleus; signal transduction; negative regulation of BMP signaling pathway; activation of NF-kappaB transcription factor; negative regulation of bone mineralization; cell-cell signaling; positive regulation of cell proliferation; negative regulation of leukocyte chemotaxis; angiogenesis; proximal/distal pattern formation; embryonic limb morphogenesis; determination of dorsal identity; positive regulation of telomerase activity; regulation of stress-activated MAPK cascade; positive regulation of angiogenesis; organ morphogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; regulation of focal adhesion formation; negative regulation of bone remodeling; endothelial cell migration; negative regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
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 Research Articles on GREM1    1. GREM1 induces fibrosis and angiogenesis in mouse peritoneum and is associated with increased solute transport in peritoneal dialysis patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with GREM1 elisa kitOrgans/Tissues associated with GREM1 elisa kit
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >24 publications with GREM1 and Fibrosis
 Carcinoma Antibodies  >22 publications with GREM1 and Carcinoma
 Congenital Abnormalities Antibodies  >19 publications with GREM1 and Congenital Abnormalities
 Colorectal Neoplasms Antibodies  >16 publications with GREM1 and Colorectal Neoplasms
 Disease Models, Animal Antibodies  >12 publications with GREM1 and Disease Models, Animal
 Adenocarcinoma Antibodies  >9 publications with GREM1 and Adenocarcinoma
 Lung Neoplasms Antibodies  >7 publications with GREM1 and Lung Neoplasms
 Inflammation Antibodies  >6 publications with GREM1 and Inflammation
 Pulmonary Fibrosis Antibodies  >6 publications with GREM1 and Pulmonary Fibrosis
 Hypertrophy Antibodies  >5 publications with GREM1 and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >27 publications with GREM1 and Embryonic Tissue
 Lung Antibodies  >24 publications with GREM1 and Lung
 Connective Tissue Antibodies  >19 publications with GREM1 and Connective Tissue
 Eye Antibodies  >11 publications with GREM1 and Eye
 Brain Antibodies  >9 publications with GREM1 and Brain
 Intestine Antibodies  >6 publications with GREM1 and Intestine
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