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GREM1 elisa kit :: Human Gremlin-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS761645
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Gremlin-1 (GREM1), ELISA Kit

★Popular Item★
 Also Known As   

Human Gremlin-1 ELISA Kit

 Product Synonym Names    GREM1 (GremLin 1)/DAND2/DRM/Cell proliferation-inducing gene 2 protein/CKTSF1B1/gremLin 1; cysteine knot superfamily; homolog/DAN domain family member 2/GREMLIN/Down-regulated in Mos-transformed cells protein/DRMM/gremLin/gremLin-1/gremLin 1-like protein/IHG-2/Increased in high glucose protein 2/increased in high glucose-2/proliferation-inducing gene 2
 Product Gene Name   

GREM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS761645 COA    COA PDF
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of GREM1 . No significant cross-reactivity or interference between GREM1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/ml
 Sensitivity    <0.094ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GREM1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GREM1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GREM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GREM1 purchase    MBS761645 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gremlin-1 (GREM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing GREM1, with an estimated sensitivity of <0.094ng/ml. The ELISA analytical biochemical technique of the MBS761645 kit is based on GREM1 antibody-GREM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GREM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GREM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GREM1 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GREM1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- GREM1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GREM1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GREM1 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of GREM1 elisa kit    GREM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS761645. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GREM1. It may not necessarily be applicable to this product.
 NCBI GI #    9506493
 NCBI GeneID    50566
 NCBI Accession #    NP_062155.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_019282.3 [Other Products]
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 UniProt Related Accession #    O35793 [Other Products]
 Molecular Weight    20,680 Da
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 NCBI Official Full Name    gremlin-1
 NCBI Official Synonym Full Names    gremlin 1
 NCBI Official Symbol    Grem1 [Similar Products]
 NCBI Official Synonym Symbols   
drm; Cktsf1b1
[Similar Products]
 NCBI Protein Information    gremlin-1
 UniProt Protein Name    Gremlin-1
 UniProt Synonym Protein Names   
Cysteine knot superfamily 1, BMP antagonist 1; Down-regulated in Mos-transformed cells protein
 Protein Family    Gremlin
 UniProt Gene Name    Grem1 [Similar Products]
 UniProt Synonym Gene Names    Cktsf1b1; Drm [Similar Products]
 UniProt Entry Name    GREM1_RAT
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 NCBI Summary for GREM1    protein may play a role in cellular growth control, viability and differentiation; high expression levels may induce apoptosis [RGD, Feb 2006]
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 UniProt Comments for GREM1    Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner (). Acts as inhibitor of monocyte chemotaxis. Can inhibit the growth or viability of normal cells but not transformed cells when is overexpressed.
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 Research Articles on GREM1    1. The therapeutic mechanism of Danshao Huaxian capsule in hepatic fibrosis may be associated with inhibition of the expression of Gremlin and up-regulation of the expression of BMP-7.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GREM1 elisa kitDiseases associated with GREM1 elisa kit
 Reference Product  PubMed Publications
 Bmp4 elisa kit  >42 publications with GREM1 and Bmp4
 Shh elisa kit  >27 publications with GREM1 and Shh
 Bmp2 elisa kit  >21 publications with GREM1 and Bmp2
 Bmp7 elisa kit  >13 publications with GREM1 and Bmp7
 Bmpr2 elisa kit  >6 publications with GREM1 and Bmpr2
 Fgf8 elisa kit  >5 publications with GREM1 and Fgf8
 Fgf4 elisa kit  >5 publications with GREM1 and Fgf4
 Fgf2 elisa kit  >5 publications with GREM1 and Fgf2
 Slit1 elisa kit  >2 publications with GREM1 and Slit1
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >30 publications with GREM1 and Fibrosis
 Kidney Diseases Antibodies  >28 publications with GREM1 and Kidney Diseases
 Carcinoma Antibodies  >24 publications with GREM1 and Carcinoma
 Congenital Abnormalities Antibodies  >23 publications with GREM1 and Congenital Abnormalities
 Colorectal Neoplasms Antibodies  >20 publications with GREM1 and Colorectal Neoplasms
 Disease Models, Animal Antibodies  >17 publications with GREM1 and Disease Models, Animal
 Inflammation Antibodies  >15 publications with GREM1 and Inflammation
 Lung Diseases Antibodies  >14 publications with GREM1 and Lung Diseases
 Lung Neoplasms Antibodies  >8 publications with GREM1 and Lung Neoplasms
 Breast Neoplasms Antibodies  >7 publications with GREM1 and Breast Neoplasms
Organs/Tissues associated with GREM1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >27 publications with GREM1 and Embryonic Tissue
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