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GDF elisa kit :: Human Growth Differentiation Factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS723879
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Growth Differentiation Factor (GDF), ELISA Kit

★Popular Item★
 Also Known As   

Human Growth Differentiation Factor ELISA Kit

 Product Gene Name   

GDF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GDF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GDF purchase    MBS723879 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Growth Differentiation Factor (GDF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing GDF, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS723879 kit is based on GDF antibody-GDF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GDF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GDF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GDF elisa kit

   For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Intended Uses: This GDF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GDF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: GDF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GDF. Standards or samples are then added to the microtiter plate wells and GDF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GDF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GDF are added to each well to “sandwich” the GDF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GDF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of GDF elisa kit    GDF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS723879. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GDF. It may not necessarily be applicable to this product.
 NCBI GI #    48314966
 NCBI GeneID    281187
 NCBI Accession #    NP_001001525.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001001525.2 [Other Products]
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 UniProt Secondary Accession #    O18829; Q564D4; Q8WNS6; Q95N97; A4IFF8 [Other Products]
 UniProt Related Accession #    O18836 [Other Products]
 Molecular Weight    42,551 Da
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 NCBI Official Full Name    growth/differentiation factor 8
 NCBI Official Synonym Full Names    myostatin<
 NCBI Official Symbol    MSTN [Similar Products]
 NCBI Official Synonym Symbols   
GDF8
[Similar Products]
 NCBI Protein Information    growth/differentiation factor 8; GDF-8; growth differentiation factor 8
 UniProt Protein Name    Growth/differentiation factor 8
 UniProt Synonym Protein Names   
Myostatin
 UniProt Gene Name    MSTN [Similar Products]
 UniProt Synonym Gene Names    GDF8; MH; GDF-8 [Similar Products]
 UniProt Entry Name    GDF8_BOVIN
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 UniProt Comments for GDF    Acts specifically as a negative regulator of skeletal muscle growth.
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 Research Articles on GDF    1. Mutations in the leader peptide of the bovine myostatin gene effectively promote the proliferation of bovine fibroblast cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with GDF elisa kitDiseases associated with GDF elisa kit
 Products by Pathway  Pathway Diagram
 Hypertrophy Model Pathway antibodies  Hypertrophy Model Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >147 publications with GDF and Nervous System Diseases
 Hypertrophy Antibodies  >144 publications with GDF and Hypertrophy
 Atrophy Antibodies  >119 publications with GDF and Atrophy
 Muscular Atrophy Antibodies  >87 publications with GDF and Muscular Atrophy
 Weight Loss Antibodies  >45 publications with GDF and Weight Loss
 Hyperplasia Antibodies  >35 publications with GDF and Hyperplasia
 Disease Models, Animal Antibodies  >33 publications with GDF and Disease Models, Animal
 Insulin Resistance Antibodies  >30 publications with GDF and Insulin Resistance
 Kidney Diseases Antibodies  >18 publications with GDF and Kidney Diseases
 Heart Diseases Antibodies  >16 publications with GDF and Heart Diseases
Organs/Tissues associated with GDF elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >688 publications with GDF and Muscle
 Brain Antibodies  >34 publications with GDF and Brain
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