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VAV3 elisa kit :: Human Guanine nucleotide exchange factor VAV3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762491
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
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 Product Name   

Guanine nucleotide exchange factor VAV3, ELISA Kit

★Popular Item★
 Also Known As   

Human Guanine nucleotide exchange factor VAV3 ELISA Kit

 Product Synonym Names    VAV3 (Guanine nucleotide exchange factor VAV3)/VAV-3
 Product Gene Name   

VAV3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS762491 COA    COA PDF
 OMIM    605541
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of VAV3 . No significant cross-reactivity or interference between VAV3 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between VAV3 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/ml
 Sensitivity    <0.094ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VAV3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VAV3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VAV3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VAV3 purchase    MBS762491 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Guanine nucleotide exchange factor VAV3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing VAV3, with an estimated sensitivity of <0.094ng/ml. The ELISA analytical biochemical technique of the MBS762491 kit is based on VAV3 antibody-VAV3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VAV3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VAV3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for VAV3 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- VAV3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- VAV3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VAV3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VAV3 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of VAV3 elisa kit    VAV3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS762491. Click to request current manual
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NCBI/Uniprot data below describe general gene information for VAV3. It may not necessarily be applicable to this product.
 NCBI GI #    120432044
 NCBI GeneID    10451
 NCBI Accession #    NP_006104.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_006113.4 [Other Products]
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 UniProt Secondary Accession #    O60498; O95230; Q9Y5X8; B1AMM0; B1APV5; B4E232; B7ZLR1; E9PQ97 [Other Products]
 UniProt Related Accession #    Q9UKW4 [Other Products]
 Molecular Weight    100,828 Da
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 NCBI Official Full Name    guanine nucleotide exchange factor VAV3 isoform 1
 NCBI Official Synonym Full Names    vav guanine nucleotide exchange factor 3
 NCBI Official Symbol    VAV3 [Similar Products]
 NCBI Protein Information    guanine nucleotide exchange factor VAV3
 UniProt Protein Name    Guanine nucleotide exchange factor VAV3
 Protein Family    Guanine nucleotide exchange factor
 UniProt Gene Name    VAV3 [Similar Products]
 UniProt Synonym Gene Names    VAV-3 [Similar Products]
 UniProt Entry Name    VAV3_HUMAN
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 NCBI Summary for VAV3    This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for VAV3    VAV3: a guanine nucleotide exchange factor (GEF) that activates RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by activated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion. Interacts with the PH domain of APS. Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Interacts with ROS1; constitutive interaction that mediates VAV3 phosphorylation. Down-regulated by EGF and TGF-beta. Four isoforms of the human protein are produced by alternative promoter. Isoform 1 and isoform 3 are widely expressed; both are expressed at very low levels in skeletal muscle. In keratinocytes, isoform 1 is less abundant than isoform 3. Isoform 3 is detected at very low levels, if any, in adrenal gland, bone marrow, spleen, fetal brain and spinal chord; in these tissues, isoform 1 is readily detectable.

Protein type: Nuclear receptor co-regulator; Adaptor/scaffold; Motility/polarity/chemotaxis; GEFs; GEFs, Rac/Rho; Actin-binding

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytosol; plasma membrane

Molecular Function: epidermal growth factor receptor binding; GTPase activator activity; guanyl-nucleotide exchange factor activity; metal ion binding; protein binding; Rac guanyl-nucleotide exchange factor activity; SH3/SH2 adaptor activity

Biological Process: angiogenesis; axon guidance; B cell receptor signaling pathway; blood coagulation; ephrin receptor signaling pathway; innate immune response; integrin-mediated signaling pathway; lamellipodium biogenesis; nerve growth factor receptor signaling pathway; neutrophil chemotaxis; platelet activation; positive regulation of apoptosis; positive regulation of B cell proliferation; positive regulation of cell adhesion; positive regulation of GTPase activity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of signal transduction; regulation of cell size; regulation of GTPase activity; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; response to DNA damage stimulus; response to drug; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway; vesicle fusion
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 Research Articles on VAV3    1. VAV3 overexpression could be a useful marker for predicting the outcomes of CRC patients and that VAV3 targeting represents a potential modality for treating CRC
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VAV3 elisa kitPathways associated with VAV3 elisa kit
 Reference Product  PubMed Publications
 RAC1 elisa kit  >36 publications with VAV3 and RAC1
 RHOA elisa kit  >19 publications with VAV3 and RHOA
 SYK elisa kit  >16 publications with VAV3 and SYK
 CDC42 elisa kit  >11 publications with VAV3 and CDC42
 PLCG1 elisa kit  >2 publications with VAV3 and PLCG1
 RHOG elisa kit  >2 publications with VAV3 and RHOG
 SHC1 elisa kit  >1 publications with VAV3 and SHC1
 LCP2 elisa kit  >1 publications with VAV3 and LCP2
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
Diseases associated with VAV3 elisa kitOrgans/Tissues associated with VAV3 elisa kit
 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >15 publications with VAV3 and Prostatic Neoplasms
 Inflammation Antibodies  >12 publications with VAV3 and Inflammation
 Cardiovascular Diseases Antibodies  >12 publications with VAV3 and Cardiovascular Diseases
 Immune System Diseases Antibodies  >10 publications with VAV3 and Immune System Diseases
 Breast Neoplasms Antibodies  >9 publications with VAV3 and Breast Neoplasms
 Hypertension Antibodies  >7 publications with VAV3 and Hypertension
 Carcinoma Antibodies  >6 publications with VAV3 and Carcinoma
 Lung Diseases Antibodies  >5 publications with VAV3 and Lung Diseases
 Lung Neoplasms Antibodies  >4 publications with VAV3 and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >4 publications with VAV3 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >18 publications with VAV3 and Prostate
 Brain Antibodies  >12 publications with VAV3 and Brain
 Liver Antibodies  >9 publications with VAV3 and Liver
 Lung Antibodies  >8 publications with VAV3 and Lung
 Kidney Antibodies  >7 publications with VAV3 and Kidney
 Eye Antibodies  >6 publications with VAV3 and Eye
 Muscle Antibodies  >6 publications with VAV3 and Muscle
 Heart Antibodies  >4 publications with VAV3 and Heart
 Intestine Antibodies  >3 publications with VAV3 and Intestine
 Lymph Node Antibodies  >3 publications with VAV3 and Lymph Node
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