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ERBB2 elisa kit :: Human HER2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824920
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HER2 (ERBB2), ELISA Kit

★Popular Item★
 Also Known As   

Human HER2 ELISA Kit

 Product Synonym Names    HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340
 Product Gene Name   

ERBB2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    phenotype 613659
 3D Structure    ModBase 3D Structure for P04626
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 Species Reactivity    Human
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 Specificity    The Human HER2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human HER2 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
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 Samples    Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates
 Sensitivity    10 pg/ml
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ERBB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ERBB2 purchase    MBS824920 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HER2 (ERBB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ERBB2, with an estimated sensitivity of 10 pg/ml. The ELISA analytical biochemical technique of the MBS824920 kit is based on ERBB2 antibody-ERBB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ERBB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ERBB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ERBB2 elisa kit

   Principle of the Assay: The Human HER2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HER2 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human HER2 coated on a 96-well plate. Standards and samples are pipetted into the wells and HER2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HER2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HER2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: HER2/neu (also known as ErbB-2) stands for "Human Epidermal growth factor Receptor 2" and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340 (cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family. The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17 (17q21-q22).
 Applications Tested/Suitable for ERBB2 elisa kit   

Sandwich ELISA (SE)

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 Typical Testing Data/Standard Curve (for reference only) of ERBB2 elisa kit    ERBB2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS824920. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ERBB2. It may not necessarily be applicable to this product.
 NCBI GI #    54792098
 NCBI GeneID    2064
 NCBI Accession #    NP_001005862.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001005862.2 [Other Products]
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 UniProt Primary Accession #    P04626 [Other Products]
 UniProt Secondary Accession #    Q14256; Q6LDV1; Q9UMK4; B2RZG3; B4DHN3; X5D2V5 [Other Products]
 UniProt Related Accession #    P04626 [Other Products]
 Molecular Weight    97,253 Da
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 NCBI Official Full Name    receptor tyrosine-protein kinase erbB-2 isoform b
 NCBI Official Synonym Full Names    v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
 NCBI Official Symbol    ERBB2 [Similar Products]
 NCBI Official Synonym Symbols   
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
[Similar Products]
 NCBI Protein Information    receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
 UniProt Protein Name    Receptor tyrosine-protein kinase erbB-2
 UniProt Synonym Protein Names   
Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen: CD340
 Protein Family    Receptor tyrosine-protein kinase
 UniProt Gene Name    ERBB2 [Similar Products]
 UniProt Synonym Gene Names    HER2; MLN19; NEU; NGL; MLN 19 [Similar Products]
 UniProt Entry Name    ERBB2_HUMAN
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 NCBI Summary for ERBB2    This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ERBB2    HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.

Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Oncoprotein; Protein kinase, TK; Membrane protein, integral; TK group; EGFR family

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: perinuclear region of cytoplasm; basolateral plasma membrane; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; cytoplasmic vesicle; nucleus; receptor complex

Molecular Function: protein C-terminus binding; protein dimerization activity; identical protein binding; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein phosphatase binding; protein binding; transmembrane receptor activity; ErbB-3 class receptor binding; protein heterodimerization activity; growth factor binding; protein-tyrosine kinase activity; ATP binding

Biological Process: myelination; axon guidance; phosphoinositide 3-kinase cascade; positive regulation of cell adhesion; peptidyl-tyrosine phosphorylation; wound healing; positive regulation of translation; nerve growth factor receptor signaling pathway; heart development; protein amino acid autophosphorylation; motor axon guidance; signal transduction; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase I promoter; positive regulation of MAP kinase activity; cell surface receptor linked signal transduction; oligodendrocyte differentiation; neuromuscular junction development; epidermal growth factor receptor signaling pathway; negative regulation of immature T cell proliferation in the thymus; regulation of microtubule-based process; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; positive regulation of cell growth; regulation of angiogenesis; peripheral nervous system development; cell proliferation; innate immune response; positive regulation of transcription from RNA polymerase III promoter; positive regulation of protein amino acid phosphorylation; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Gastric Cancer; Lung Cancer; Glioma Susceptibility 1
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 Research Articles on ERBB2    1. In HER2-positive breast cancers, HR expression is associated with a poor prognosis despite the hormone therapy. For this population, a personalized management may be required.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ERBB2 elisa kitPathways associated with ERBB2 elisa kit
 Reference Product  PubMed Publications
 EGFR elisa kit  >2909 publications with ERBB2 and EGFR
 ERBB3 elisa kit  >806 publications with ERBB2 and ERBB3
 SRC elisa kit  >392 publications with ERBB2 and SRC
 GRB2 elisa kit  >109 publications with ERBB2 and GRB2
 GRB7 elisa kit  >81 publications with ERBB2 and GRB7
 ERBB2IP elisa kit  >42 publications with ERBB2 and ERBB2IP
 SHC1 elisa kit  >35 publications with ERBB2 and SHC1
 UBC elisa kit  >3 publications with ERBB2 and UBC
 HSP90AA1 elisa kit  >1 publications with ERBB2 and HSP90AA1
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
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 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
Diseases associated with ERBB2 elisa kitOrgans/Tissues associated with ERBB2 elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >12263 publications with ERBB2 and Breast Neoplasms
 Skin Diseases Antibodies  >11404 publications with ERBB2 and Skin Diseases
 Carcinoma Antibodies  >6646 publications with ERBB2 and Carcinoma
 Adenocarcinoma Antibodies  >3505 publications with ERBB2 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >2889 publications with ERBB2 and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >1684 publications with ERBB2 and Neoplasms, Experimental
 Disease Progression Antibodies  >1608 publications with ERBB2 and Disease Progression
 Lymphatic Metastasis Antibodies  >1324 publications with ERBB2 and Lymphatic Metastasis
 Gastrointestinal Neoplasms Antibodies  >1291 publications with ERBB2 and Gastrointestinal Neoplasms
 Lung Neoplasms Antibodies  >1176 publications with ERBB2 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2182 publications with ERBB2 and Blood
 Lymph Node Antibodies  >1913 publications with ERBB2 and Lymph Node
 Lung Antibodies  >1392 publications with ERBB2 and Lung
 Stomach Antibodies  >762 publications with ERBB2 and Stomach
 Brain Antibodies  >653 publications with ERBB2 and Brain
 Liver Antibodies  >576 publications with ERBB2 and Liver
 Prostate Antibodies  >503 publications with ERBB2 and Prostate
 Bone Antibodies  >474 publications with ERBB2 and Bone
 Mammary Gland Antibodies  >452 publications with ERBB2 and Mammary Gland
 Heart Antibodies  >425 publications with ERBB2 and Heart
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