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HIF-1alpha elisa kit :: Human HIF-1alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2503734 (PROMOTIONAL PRICING: 20% OFF web price.
Discount will be applied after order has been placed)
HIF-1alpha elisa kit
Unit / Price
96-Strip-Wells  /  $445 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HIF-1alpha, ELISA Kit

 Also Known As   

Human HIF-1alpha (Hypoxia Inducible Factor 1 Alpha) ELISA Kit

 Product Gene Name   

HIF-1alpha elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS2503734 COA    COA PDF
 OMIM    603348
 3D Structure    ModBase 3D Structure for Q16665
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human HIF-1alpha. No significant cross-reactivity or interference between Human HIF-1alpha and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.094ng/mL
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 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of HIF-1alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIF-1alpha purchase    MBS2503734 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HIF-1alpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing HIF-1alpha, with an estimated sensitivity of 0.094ng/mL. The ELISA analytical biochemical technique of the MBS2503734 kit is based on HIF-1alpha antibody-HIF-1alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF-1alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF-1alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HIF-1alpha elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human HIF-1alpha concentrations in serum, plasma and other biological fluids.

Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HIF-1alpha. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HIF-1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HIF-1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HIF-1alpha. You can calculate the concentration of HIF-1alpha in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2503734
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NCBI/Uniprot data below describe general gene information for HIF-1alpha. It may not necessarily be applicable to this product.
 NCBI GI #    4504385
 NCBI GeneID    3091
 NCBI Accession #    NP_001521.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001530.3 [Other Products]
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 UniProt Primary Accession #    Q16665 [Other Products]
 UniProt Secondary Accession #    Q53XP6; Q96PT9; Q9UPB1; C0LZJ3 [Other Products]
 UniProt Related Accession #    Q16665 [Other Products]
 Molecular Weight    95,634 Da
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 NCBI Official Full Name    hypoxia-inducible factor 1-alpha isoform 1
 NCBI Official Synonym Full Names    hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 NCBI Official Symbol    HIF1A [Similar Products]
 NCBI Official Synonym Symbols   
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
[Similar Products]
 NCBI Protein Information    hypoxia-inducible factor 1-alpha; ARNT interacting protein; ARNT-interacting protein; HIF-1-alpha; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1
 UniProt Protein Name    Hypoxia-inducible factor 1-alpha
 UniProt Synonym Protein Names   
ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8
 UniProt Gene Name    HIF1A [Similar Products]
 UniProt Synonym Gene Names    BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78 [Similar Products]
 UniProt Entry Name    HIF1A_HUMAN
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 NCBI Summary for HIF-1alpha    This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
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 UniProt Comments for HIF-1alpha    HIF1A: a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability. Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia- mimicking conditions. Interacts with USP20. Interacts with RACK1; promotes HIF1A ubiquitination and proteasome- mediated degradation. Interacts (via N-terminus) with USP19. Under reduced oxygen tension. Induced also by various receptor-mediated factors such as growth factors, cytokines, and circulatory factors such as PDGF, EGF, FGF2, IGF2, TGFB1, HGF, TNF, IL1B, angiotensin-2 and thrombin. However, this induction is less intense than that stimulated by hypoxia. Repressed by HIPK2 and LIMD1. Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nuclear speck; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone deacetylase binding; Hsp90 protein binding; protein kinase binding; transcription factor binding; histone acetyltransferase binding; protein binding; signal transducer activity; enzyme binding; sequence-specific DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription factor activity; nuclear hormone receptor binding

Biological Process: lactation; oxygen homeostasis; response to muscle activity; embryonic placenta development; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; glucose homeostasis; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; muscle maintenance; negative regulation of bone mineralization; connective tissue replacement during inflammatory response; elastin metabolic process; axon transport of mitochondrion; regulation of transcription, DNA-dependent; visual learning; angiogenesis; heart looping; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; neural crest cell migration; negative regulation of growth; hemoglobin biosynthetic process; positive regulation of neuroblast proliferation; regulation of transforming growth factor-beta2 production; Notch signaling pathway; negative regulation of TOR signaling pathway; collagen metabolic process; embryonic hemopoiesis; positive regulation of erythrocyte differentiation; positive regulation of nitric-oxide synthase activity; B-1 B cell homeostasis; digestive tract morphogenesis; mRNA transcription from RNA polymerase II promoter; positive regulation of chemokine production; positive regulation of angiogenesis; neural fold elevation formation; regulation of gene expression; positive regulation of hormone biosynthetic process; cartilage development; lactate metabolic process; positive regulation of glycolysis; response to hypoxia; epithelial to mesenchymal transition; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development
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 Research Articles on HIF-1alpha    1. HIF-1alpha is an important player in multiple myeloma homeostasis and that its inhibition by small antisense oligonucleotides provides a rationale for novel therapeutic strategy to improving MM treatment.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIF-1alpha elisa kitPathways associated with HIF-1alpha elisa kit
 Reference Product  PubMed Publications
 VHL elisa kit  >528 publications with HIF-1alpha and VHL
 ARNT elisa kit  >292 publications with HIF-1alpha and ARNT
 EGLN1 elisa kit  >143 publications with HIF-1alpha and EGLN1
 EGLN3 elisa kit  >67 publications with HIF-1alpha and EGLN3
 HIF1AN elisa kit  >59 publications with HIF-1alpha and HIF1AN
 TP53 elisa kit  >46 publications with HIF-1alpha and TP53
 EP300 elisa kit  >28 publications with HIF-1alpha and EP300
 CREBBP elisa kit  >14 publications with HIF-1alpha and CREBBP
 UBC elisa kit  >4 publications with HIF-1alpha and UBC
 HSP90AA1 elisa kit  >3 publications with HIF-1alpha and HSP90AA1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
Diseases associated with HIF-1alpha elisa kitOrgans/Tissues associated with HIF-1alpha elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1457 publications with HIF-1alpha and Carcinoma
 Nervous System Diseases Antibodies  >700 publications with HIF-1alpha and Nervous System Diseases
 Inflammation Antibodies  >560 publications with HIF-1alpha and Inflammation
 Brain Diseases Antibodies  >515 publications with HIF-1alpha and Brain Diseases
 Lung Diseases Antibodies  >456 publications with HIF-1alpha and Lung Diseases
 Kidney Diseases Antibodies  >447 publications with HIF-1alpha and Kidney Diseases
 Breast Neoplasms Antibodies  >444 publications with HIF-1alpha and Breast Neoplasms
 Liver Diseases Antibodies  >389 publications with HIF-1alpha and Liver Diseases
 Necrosis Antibodies  >386 publications with HIF-1alpha and Necrosis
 Neoplasms, Experimental Antibodies  >354 publications with HIF-1alpha and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >3398 publications with HIF-1alpha and Vascular
 Blood Antibodies  >1993 publications with HIF-1alpha and Blood
 Brain Antibodies  >769 publications with HIF-1alpha and Brain
 Muscle Antibodies  >763 publications with HIF-1alpha and Muscle
 Kidney Antibodies  >716 publications with HIF-1alpha and Kidney
 Lung Antibodies  >656 publications with HIF-1alpha and Lung
 Liver Antibodies  >618 publications with HIF-1alpha and Liver
 Heart Antibodies  >571 publications with HIF-1alpha and Heart
 Bone Antibodies  >456 publications with HIF-1alpha and Bone
 Prostate Antibodies  >293 publications with HIF-1alpha and Prostate
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