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HIF3A elisa kit :: Human HIF3A (Hypoxia-inducible factor 3-alpha) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7606705
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HIF3A (Hypoxia-inducible factor 3-alpha), ELISA Kit

★Popular Item★
 Also Known As   

Human HIF3A (Hypoxia-inducible factor 3-alpha) ELISA Kit

 Product Gene Name   

HIF3A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS7606705 COA    COA PDF
 3D Structure    ModBase 3D Structure for Q0VBL6
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of HIF3A . No significant cross-reactivity or interference between HIF3A and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    78-5000pg/ml
 Sensitivity    <46.9pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HIF3A were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HIF3A were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HIF3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIF3A purchase    MBS7606705 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HIF3A (Hypoxia-inducible factor 3-alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78-5000pg/ml in biological research samples containing HIF3A, with an estimated sensitivity of <46.9pg/ml. The ELISA analytical biochemical technique of the MBS7606705 kit is based on HIF3A antibody-HIF3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HIF3A elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HIF3A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HIF3A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HIF3A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HIF3A can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of HIF3A elisa kit    HIF3A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7606705. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HIF3A. It may not necessarily be applicable to this product.
 NCBI GI #    251823727
 NCBI GeneID    53417
 NCBI Accession #    NP_001156422.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001162950.1 [Other Products]
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 UniProt Primary Accession #    Q0VBL6 [Other Products]
 UniProt Secondary Accession #    Q3UN40; Q8VHR1; Q9QX54; Q9Z2I5; A1IM61; E9QLB1 [Other Products]
 UniProt Related Accession #    Q0VBL6 [Other Products]
 Molecular Weight    73,122 Da
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 NCBI Official Full Name    hypoxia-inducible factor 3-alpha isoform 1
 NCBI Official Synonym Full Names    hypoxia inducible factor 3, alpha subunit
 NCBI Official Symbol    Hif3a [Similar Products]
 NCBI Official Synonym Symbols   
Ipas; MOP7; NEPAS; bHLHe17
[Similar Products]
 NCBI Protein Information    hypoxia-inducible factor 3-alpha
 UniProt Protein Name    Hypoxia-inducible factor 3-alpha
 UniProt Synonym Protein Names   
Basic-helix-loop-helix-PAS protein MOP7; HIF3-alpha-1; Inhibitory PAS domain protein
 Protein Family    Hypoxia-inducible factor
 UniProt Gene Name    Hif3a [Similar Products]
 UniProt Synonym Gene Names    IPAS [Similar Products]
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 UniProt Comments for HIF3A    HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 7|7 A2

Cellular Component: cytoplasm; cytosol; mitochondrion; nuclear speck; nucleoplasm; nucleus; plasma membrane

Molecular Function: DNA binding; protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; response to hypoxia; transcription from RNA polymerase II promoter
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 Research Articles on HIF3A    1. Report suggests that BV-2 microglial cells express HIF-3a under inflammatory conditions and that its activation differed from that of HIF-1a
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIF3A elisa kitPathways associated with HIF3A elisa kit
 Reference Product  PubMed Publications
 Arnt elisa kit  >16 publications with HIF3A and Arnt
 Vegfa elisa kit  >9 publications with HIF3A and Vegfa
 Vhl elisa kit  >6 publications with HIF3A and Vhl
 Crebbp elisa kit  >1 publications with HIF3A and Crebbp
 Products by Pathway  Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway antibodies  Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway Diagram
 Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway antibodies  Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway Diagram
Diseases associated with HIF3A elisa kitOrgans/Tissues associated with HIF3A elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >8 publications with HIF3A and Cardiovascular Diseases
 Kidney Diseases Antibodies  >6 publications with HIF3A and Kidney Diseases
 Lung Diseases Antibodies  >5 publications with HIF3A and Lung Diseases
 Hypertension Antibodies  >5 publications with HIF3A and Hypertension
 Heart Diseases Antibodies  >4 publications with HIF3A and Heart Diseases
 Hypertrophy Antibodies  >4 publications with HIF3A and Hypertrophy
 Carcinoma Antibodies  >4 publications with HIF3A and Carcinoma
 Liver Diseases Antibodies  >3 publications with HIF3A and Liver Diseases
 Congenital Abnormalities Antibodies  >2 publications with HIF3A and Congenital Abnormalities
 Myocardial Infarction Antibodies  >2 publications with HIF3A and Myocardial Infarction
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >17 publications with HIF3A and Lung
 Kidney Antibodies  >10 publications with HIF3A and Kidney
 Brain Antibodies  >9 publications with HIF3A and Brain
 Eye Antibodies  >4 publications with HIF3A and Eye
 Embryonic Tissue Antibodies  >4 publications with HIF3A and Embryonic Tissue
 Bone Marrow Antibodies  >2 publications with HIF3A and Bone Marrow
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