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HLA-DRB1-9 elisa kit :: Human HLA class II histocompatibility antigen, DRB1-9 beta chain ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2887133
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $560 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,445 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,520 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HLA class II histocompatibility antigen, DRB1-9 beta chain (HLA-DRB1-9), ELISA Kit

★Popular Item★
 Also Known As   

Human HLA class II histocompatibility antigen, DRB1-9 beta chain ELISA Kit

 Product Synonym Names    HLA class II histocompatibility antigen, DRB1-16 beta chain; MHC class II antigen DRB1*16; DR-16; DR16; HLA-DRB1
 Product Gene Name   

HLA-DRB1-9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2887133 Testing Data    Testing Data PDF
 OMIM    142857
 3D Structure    ModBase 3D Structure for Q29974
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 Species Reactivity    Human
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 Specificity    Recombinant and natural Human HLA class II histocompatibility antigen, DRB1-9 beta chain
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
 Detection Range    0.625-40 ng/mL
 Intra-assay Precision    Intra-Assay CV: <=4.4%
 Inter-assay Precision    Inter-Assay CV: <=5.8%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HLA-DRB1-9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HLA-DRB1-9 purchase    MBS2887133 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HLA class II histocompatibility antigen, DRB1-9 beta chain (HLA-DRB1-9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40 ng/mL in biological research samples containing HLA-DRB1-9. The ELISA analytical biochemical technique of the MBS2887133 kit is based on HLA-DRB1-9 antibody-HLA-DRB1-9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HLA-DRB1-9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HLA-DRB1-9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HLA-DRB1-9 elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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  Typical Testing Data/Standard Curve (for reference only) of HLA-DRB1-9 elisa kit    HLA-DRB1-9 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2887133. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HLA-DRB1-9. It may not necessarily be applicable to this product.
 NCBI GI #    155030211
 NCBI GeneID    3123
 NCBI Accession #    NP_002115.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002124.3 [Other Products]
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 UniProt Primary Accession #    Q29974 [Other Products]
 UniProt Secondary Accession #    O98212; Q0PGR5; Q29792; Q30120; Q30159; Q30200; Q3HUP9; Q3KTM1; Q3LA84; Q6T865; A7X5J4 [Other Products]
 UniProt Related Accession #    P20039; Q5Y7A7; Q95IE3; Q9GIY3; Q29974; P01912; Q30134; P04229; P01911; Q30167 [Other Products]
 Molecular Weight    30,030 Da
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 NCBI Official Full Name    major histocompatibility complex, class II, DR beta 1
 NCBI Official Synonym Full Names    major histocompatibility complex, class II, DR beta 1
 NCBI Official Symbol    HLA-DRB1 [Similar Products]
 NCBI Official Synonym Symbols   
SS1; DRB1; DRw10; HLA-DRB; HLA-DR1B
[Similar Products]
 NCBI Protein Information    major histocompatibility complex, class II, DR beta 1
 UniProt Protein Name    HLA class II histocompatibility antigen, DRB1-16 beta chain
 UniProt Synonym Protein Names   
MHC class II antigen DRB1*16; DR-16; DR16
 Protein Family    HLA class II histocompatibility antigen
 UniProt Gene Name    HLA-DRB1 [Similar Products]
 UniProt Synonym Gene Names    DR-16; DR16 [Similar Products]
 UniProt Entry Name    2B1G_HUMAN
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 NCBI Summary for HLA-DRB1-9    HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HLA-DRB1-9    Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
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 Research Articles on HLA-DRB1-9    1. genetic polymorphism is associated with recurrent miscarriage; meta-analysis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HLA-DRB1-9 elisa kitPathways associated with HLA-DRB1-9 elisa kit
 Reference Product  PubMed Publications
 CD4 elisa kit  >522 publications with HLA-DRB1-9 and CD4
 CD80 elisa kit  >15 publications with HLA-DRB1-9 and CD80
 CD86 elisa kit  >12 publications with HLA-DRB1-9 and CD86
 ICAM1 elisa kit  >11 publications with HLA-DRB1-9 and ICAM1
 CIITA elisa kit  >10 publications with HLA-DRB1-9 and CIITA
 IFNG elisa kit  >7 publications with HLA-DRB1-9 and IFNG
 CD74 elisa kit  >4 publications with HLA-DRB1-9 and CD74
 PDCD1 elisa kit  >3 publications with HLA-DRB1-9 and PDCD1
 VCAM1 elisa kit  >2 publications with HLA-DRB1-9 and VCAM1
 IRF8 elisa kit  >1 publications with HLA-DRB1-9 and IRF8
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
Diseases associated with HLA-DRB1-9 elisa kitOrgans/Tissues associated with HLA-DRB1-9 elisa kit
 Disease Name  Pubmed Publications
 Arthritis, Rheumatoid Antibodies  >1233 publications with HLA-DRB1-9 and Arthritis, Rheumatoid
 Nervous System Diseases Antibodies  >929 publications with HLA-DRB1-9 and Nervous System Diseases
 Multiple Sclerosis Antibodies  >616 publications with HLA-DRB1-9 and Multiple Sclerosis
 Diabetes Mellitus, Type 1 Antibodies  >586 publications with HLA-DRB1-9 and Diabetes Mellitus, Type 1
 Necrosis Antibodies  >366 publications with HLA-DRB1-9 and Necrosis
 Liver Diseases Antibodies  >363 publications with HLA-DRB1-9 and Liver Diseases
 Inflammation Antibodies  >254 publications with HLA-DRB1-9 and Inflammation
 Hypersensitivity Antibodies  >221 publications with HLA-DRB1-9 and Hypersensitivity
 Gastrointestinal Diseases Antibodies  >219 publications with HLA-DRB1-9 and Gastrointestinal Diseases
 Lupus Erythematosus, Systemic Antibodies  >212 publications with HLA-DRB1-9 and Lupus Erythematosus, Systemic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2638 publications with HLA-DRB1-9 and Blood
 Bone Antibodies  >567 publications with HLA-DRB1-9 and Bone
 Bone Marrow Antibodies  >493 publications with HLA-DRB1-9 and Bone Marrow
 Liver Antibodies  >297 publications with HLA-DRB1-9 and Liver
 Kidney Antibodies  >234 publications with HLA-DRB1-9 and Kidney
 Skin Antibodies  >198 publications with HLA-DRB1-9 and Skin
 Lung Antibodies  >152 publications with HLA-DRB1-9 and Lung
 Thyroid Antibodies  >127 publications with HLA-DRB1-9 and Thyroid
 Brain Antibodies  >117 publications with HLA-DRB1-9 and Brain
 Heart Antibodies  >110 publications with HLA-DRB1-9 and Heart
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