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HB elisa kit :: Human Hemoglobin (HB) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS455796
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
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 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hemoglobin (HB), ELISA Kit

★Popular Item★
 Also Known As   

Human Hemoglobin (HB) ELISA Kit

 Product Synonym Names    Hgb; Haemoglobin; Heterotetramer(αβ)2
 Product Gene Name   

HB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9FY42
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of HB. No significant cross-reactivity or interference between HB and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    3.125-200ng/mL
 Sensitivity    1.31ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HB were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HB were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HB purchase    MBS455796 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hemoglobin (HB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.125-200ng/mL in biological research samples containing HB, with an estimated sensitivity of 1.31ng/mL. The ELISA analytical biochemical technique of the MBS455796 kit is based on HB antibody-HB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HB elisa kit

   Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HB in human serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of HB elisa kit    HB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS455796. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HB. It may not necessarily be applicable to this product.
 NCBI GI #    162462053
 NCBI GeneID    541815
 NCBI Accession #    NP_001104966.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001111496.1 [Other Products]
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 UniProt Primary Accession #    Q9FY42 [Other Products]
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 NCBI Official Full Name    hemoglobin
 NCBI Official Symbol    LOC541815 [Similar Products]
 NCBI Official Synonym Symbols   
GRMZM2G067402
[Similar Products]
 NCBI Protein Information    hemoglobin
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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