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HMWK elisa kit :: Human High Molecular Weight Kininogen (HMWK) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019405 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $355 +1 FREE 8GB USB
48-Strip-Wells  /  $540 +1 FREE 8GB USB
96-Strip-Wells  /  $730 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,985 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,410 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

High Molecular Weight Kininogen (HMWK), ELISA Kit

 Also Known As   

High Molecular Weight Kininogen (HMWK) ELISA Kit

 Product Synonym Names    Williams-Fitzgerald-Flaujeac Factor; Fitzgerald Factor; HMWK-Kallikrein Factor
 Product Gene Name   

HMWK elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 3D Structure    ModBase 3D Structure for O08677
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of High Molecular Weight Kininogen (HMWK).
No significant cross-reactivity or interference between High Molecular Weight Kininogen (HMWK) and analogues was observed.
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 Samples    Plasma
 Assay Type    Sandwich
 Detection Range    0.625-40ng/mL
 Sensitivity    Typically less than 0.287ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Molecular Weight Kininogen (HMWK) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Molecular Weight Kininogen (HMWK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of HMWK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HMWK purchase    MBS2019405 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the High Molecular Weight Kininogen (HMWK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40ng/mL in biological research samples containing HMWK, with an estimated sensitivity of Typically less than 0.287ng/mL. The ELISA analytical biochemical technique of the MBS2019405 kit is based on HMWK antibody-HMWK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HMWK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HMWK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HMWK elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Molecular Weight Kininogen (HMWK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Molecular Weight Kininogen (HMWK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Molecular Weight Kininogen (HMWK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Molecular Weight Kininogen (HMWK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of HMWK elisa kit    HMWK elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019405
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NCBI/Uniprot data below describe general gene information for HMWK. It may not necessarily be applicable to this product.
 NCBI GI #    50082914
 NCBI GeneID    16644
 NCBI Accession #    AAT70087.1 [Other Products]
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 UniProt Primary Accession #    O08677 [Other Products]
 UniProt Secondary Accession #    O08676; Q32MX7; Q6S9I1; Q91XK5 [Other Products]
 UniProt Related Accession #    O08677 [Other Products]
 Molecular Weight    53,206 Da
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 NCBI Official Full Name    high molecular weight kininogen I isoform DeltaD5
 NCBI Official Synonym Full Names    kininogen 1
 NCBI Official Symbol    Kng1 [Similar Products]
 NCBI Official Synonym Symbols   
Kng
[Similar Products]
 NCBI Protein Information    kininogen-1
 UniProt Protein Name    Kininogen-1
 UniProt Gene Name    Kng1 [Similar Products]
 UniProt Synonym Gene Names    Kng [Similar Products]
 UniProt Entry Name    KNG1_MOUSE
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 UniProt Comments for HMWK    KNG1: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency). HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cell adhesion; Secreted; Inhibitor; Motility/polarity/chemotaxis; Contractile

Cellular Component: extracellular space; extracellular region

Molecular Function: protease inhibitor activity; cysteine protease inhibitor activity; receptor binding

Biological Process: hemostasis; negative regulation of peptidase activity; blood coagulation; inflammatory response; regulation of blood vessel size; vasodilation
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 Research Articles on HMWK    1. uPAR mediates efferocytosis through high molecular weight kininogen interaction with phosphatidylserine on apoptotic cells
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with HMWK elisa kitDiseases associated with HMWK elisa kit
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >9 publications with HMWK and Cardiovascular Diseases
 Heart Diseases Antibodies  >5 publications with HMWK and Heart Diseases
 Thrombosis Antibodies  >5 publications with HMWK and Thrombosis
 Atherosclerosis Antibodies  >4 publications with HMWK and Atherosclerosis
 Inflammation Antibodies  >2 publications with HMWK and Inflammation
 Carcinoma, Hepatocellular Antibodies  >2 publications with HMWK and Carcinoma, Hepatocellular
 Seizures Antibodies  >1 publications with HMWK and Seizures
 Liver Diseases Antibodies  >1 publications with HMWK and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with HMWK and Kidney Diseases
 Hypertension Antibodies  >1 publications with HMWK and Hypertension
Organs/Tissues associated with HMWK elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2 publications with HMWK and Liver
 Eye Antibodies  >1 publications with HMWK and Eye
 Muscle Antibodies  >1 publications with HMWK and Muscle
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