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HTRA1 elisa kit :: Human HtrA serine peptidase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS902316
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HtrA serine peptidase 1 (HTRA1), ELISA Kit

★Popular Item★
 Also Known As   

Human Serine protease HTRA1, HTRA1 ELISA Kit

 Product Synonym Names    Human Serine protease HTRA1 (HTRA1) ELISA kit; ARMD7; HtrA; L56; ORF480; PRSS11; IGFBP5-protease; protease; serine; 11 (IGF binding) ; HtrA serine peptidase 1
 Product Gene Name   

HTRA1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS902316 Testing Data    Testing Data PDF
 OMIM    gene 610149
 3D Structure    ModBase 3D Structure for Q92743
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human HTRA1. No significant cross-reactivity or interference between human HTRA1 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates
 Assay Type    Quantitative Sandwich
 Detection Range    1.56 ng/ml -100 ng/ml
 Sensitivity    Typically less than 0.39 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HTRA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HTRA1 purchase    MBS902316 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HtrA serine peptidase 1 (HTRA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 ng/ml -100 ng/ml in biological research samples containing HTRA1, with an estimated sensitivity of Typically less than 0.39 ng/ml. The ELISA analytical biochemical technique of the MBS902316 kit is based on HTRA1 antibody-HTRA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTRA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTRA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HTRA1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HTRA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HTRA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTRA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTRA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HTRA1 elisa kit    HTRA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS902316. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HTRA1. It may not necessarily be applicable to this product.
 NCBI GI #    4506141
 NCBI GeneID    5654
 NCBI Accession #    NP_002766.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002775.4 [Other Products]
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 UniProt Primary Accession #    Q92743 [Other Products]
 UniProt Secondary Accession #    Q9UNS5; D3DRE4 [Other Products]
 UniProt Related Accession #    Q92743 [Other Products]
 Molecular Weight    51,287 Da
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 NCBI Official Full Name    serine protease HTRA1
 NCBI Official Synonym Full Names    HtrA serine peptidase 1
 NCBI Official Symbol    HTRA1 [Similar Products]
 NCBI Official Synonym Symbols   
L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL
[Similar Products]
 NCBI Protein Information    serine protease HTRA1; IGFBP5-protease; protease, serine, 11 (IGF binding); high-temperature requirement A serine peptidase 1
 UniProt Protein Name    Serine protease HTRA1
 UniProt Synonym Protein Names   
High-temperature requirement A serine peptidase 1; L56; Serine protease 11
 Protein Family    Serine protease
 UniProt Gene Name    HTRA1 [Similar Products]
 UniProt Synonym Gene Names    HTRA; PRSS11 [Similar Products]
 UniProt Entry Name    HTRA1_HUMAN
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 NCBI Summary for HTRA1    This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HTRA1    HTRA1: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians. Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, atherosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. Belongs to the peptidase S1B family.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: extracellular matrix; extracellular space; cytosol

Molecular Function: insulin-like growth factor binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of defense response to virus; regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; proteolysis; positive regulation of epithelial cell proliferation; negative regulation of BMP signaling pathway

Disease: Macular Degeneration, Age-related, 7; Cerebral Autosomal Recessive Arteriopathy With Subcortical Infarcts And Leukoencephalopathy
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 Research Articles on HTRA1    1. the inhibitory effect of IFN-gamma on HTRA1 expression was evidenced in collagen-induced arthritis (CIA) mouse models and in human RA synovial cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HTRA1 elisa kitDiseases associated with HTRA1 elisa kit
 Reference Product  PubMed Publications
 COL2A1 elisa kit  >2 publications with HTRA1 and COL2A1
 BMP4 elisa kit  >2 publications with HTRA1 and BMP4
 GDF5 elisa kit  >1 publications with HTRA1 and GDF5
 COL3A1 elisa kit  >1 publications with HTRA1 and COL3A1
 COL1A1 elisa kit  >1 publications with HTRA1 and COL1A1
 Disease Name  Pubmed Publications
 Macular Degeneration Antibodies  >143 publications with HTRA1 and Macular Degeneration
 Nervous System Diseases Antibodies  >21 publications with HTRA1 and Nervous System Diseases
 Neoplasms Antibodies  >20 publications with HTRA1 and Neoplasms
 Brain Diseases Antibodies  >13 publications with HTRA1 and Brain Diseases
 Genital Neoplasms, Female Antibodies  >8 publications with HTRA1 and Genital Neoplasms, Female
 Vision Disorders Antibodies  >6 publications with HTRA1 and Vision Disorders
 Neoplasms, Experimental Antibodies  >6 publications with HTRA1 and Neoplasms, Experimental
 Inflammation Antibodies  >6 publications with HTRA1 and Inflammation
 Hypertension Antibodies  >5 publications with HTRA1 and Hypertension
 Skin Diseases Antibodies  >5 publications with HTRA1 and Skin Diseases
Organs/Tissues associated with HTRA1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >85 publications with HTRA1 and Eye
 Vascular Antibodies  >38 publications with HTRA1 and Vascular
 Blood Antibodies  >30 publications with HTRA1 and Blood
 Ear Antibodies  >14 publications with HTRA1 and Ear
 Bone Antibodies  >14 publications with HTRA1 and Bone
 Brain Antibodies  >14 publications with HTRA1 and Brain
 Placenta Antibodies  >12 publications with HTRA1 and Placenta
 Embryonic Tissue Antibodies  >8 publications with HTRA1 and Embryonic Tissue
 Uterus Antibodies  >6 publications with HTRA1 and Uterus
 Connective Tissue Antibodies  >5 publications with HTRA1 and Connective Tissue
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