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HTT elisa kit :: Human Huntingtin (HTT) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021852 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Huntingtin (HTT), ELISA Kit

★Popular Item★
 Also Known As   

Huntingtin (HTT) ELISA Kit

 Product Synonym Names    IT15; HD; Interesting Transcript 15; Huntington disease protein
 Product Gene Name   

HTT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    143100
 3D Structure    ModBase 3D Structure for P42858
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Huntingtin (HTT).
No significant cross-reactivity or interference between Huntingtin (HTT) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.063ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Huntingtin (HTT) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Huntingtin (HTT) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HTT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HTT purchase    MBS2021852 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Huntingtin (HTT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing HTT, with an estimated sensitivity of < 0.063ng/mL. The ELISA analytical biochemical technique of the MBS2021852 kit is based on HTT antibody-HTT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HTT elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Huntingtin (HTT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Huntingtin (HTT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Huntingtin (HTT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Huntingtin (HTT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for HTT elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of HTT elisa kit    HTT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021852. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HTT. It may not necessarily be applicable to this product.
 NCBI GI #    90903231
 NCBI GeneID    3064
 NCBI Accession #    NP_002102.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_002111.7 [Other Products]
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 UniProt Primary Accession #    P42858 [Other Products]
 UniProt Secondary Accession #    Q9UQB7 [Other Products]
 UniProt Related Accession #    P42858 [Other Products]
 Molecular Weight    347,603 Da
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 NCBI Official Full Name    huntingtin
 NCBI Official Synonym Full Names    huntingtin
 NCBI Official Symbol    HTT [Similar Products]
 NCBI Official Synonym Symbols   
HD; IT15
[Similar Products]
 NCBI Protein Information    huntingtin
 UniProt Protein Name    Huntingtin
 UniProt Synonym Protein Names   
Huntington disease protein; HD protein
 Protein Family    HD protein
 UniProt Gene Name    HTT [Similar Products]
 UniProt Synonym Gene Names    HD; IT15; HD protein [Similar Products]
 UniProt Entry Name    HD_HUMAN
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 NCBI Summary for HTT    Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HTT    Huntingtin: may play a role in microtubule-mediated transport or vesicle function. Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. Defects are the cause of Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the Huntingtin gene, which translates as a polyglutamine repeat in the protein product. The Huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The Huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated isoforms displaying different relative abundance in various fetal and adult tissues.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: Golgi apparatus; cytoplasmic vesicle membrane; protein complex; mitochondrion; endoplasmic reticulum; dendrite; autophagic vacuole; inclusion body; cytosol; nucleoplasm; axon; late endosome; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; p53 binding; dynein intermediate chain binding; beta-tubulin binding; diazepam binding; transcription factor binding

Biological Process: ER to Golgi vesicle-mediated transport; paraxial mesoderm formation; citrulline metabolic process; regulation of protein phosphatase type 2A activity; regulation of synaptic plasticity; locomotory behavior; determination of adult life span; endosome transport; anterior/posterior pattern formation; L-glutamate import; establishment of mitotic spindle orientation; regulation of mitochondrial membrane potential; protein import into nucleus; organ development; quinolinate biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; vesicle transport along microtubule; visual learning; negative regulation of neuron apoptosis; Golgi organization and biogenesis; grooming behavior; endoplasmic reticulum organization and biogenesis; positive regulation of inositol-1,4,5-triphosphate receptor activity; striatum development; axon cargo transport; cell aging; olfactory lobe development; social behavior; lactate biosynthetic process from pyruvate; neuron apoptosis; iron ion homeostasis; insulin secretion; dopamine receptor signaling pathway; hormone metabolic process; neuron development; spermatogenesis; regulation of mitochondrial membrane permeability; response to calcium ion; neural plate formation; urea cycle

Disease: Huntington Disease
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 Research Articles on HTT    1. show that essential, species-relevant functions are disrupted by the HD mutation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HTT elisa kitPathways associated with HTT elisa kit
 Reference Product  PubMed Publications
 HAP1 elisa kit  >35 publications with HTT and HAP1
 HIP1 elisa kit  >24 publications with HTT and HIP1
 UBC elisa kit  >14 publications with HTT and UBC
 CREBBP elisa kit  >11 publications with HTT and CREBBP
 ZDHHC17 elisa kit  >8 publications with HTT and ZDHHC17
 GAPDH elisa kit  >6 publications with HTT and GAPDH
 SH3GL3 elisa kit  >4 publications with HTT and SH3GL3
 OPTN elisa kit  >4 publications with HTT and OPTN
 PACSIN1 elisa kit  >4 publications with HTT and PACSIN1
 PRPF40A elisa kit  >1 publications with HTT and PRPF40A
 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
Diseases associated with HTT elisa kitOrgans/Tissues associated with HTT elisa kit
 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >1550 publications with HTT and Neurodegenerative Diseases
 Huntington Disease Antibodies  >1540 publications with HTT and Huntington Disease
 Brain Diseases Antibodies  >1511 publications with HTT and Brain Diseases
 Congenital, Hereditary, and Neonatal Diseases and Abnormalities Antibodies  >1434 publications with HTT and Congenital, Hereditary, and Neonatal Diseases and Abnormalities
 Cognition Disorders Antibodies  >1412 publications with HTT and Cognition Disorders
 Movement Disorders Antibodies  >1412 publications with HTT and Movement Disorders
 Nerve Degeneration Antibodies  >181 publications with HTT and Nerve Degeneration
 Anxiety Disorders Antibodies  >177 publications with HTT and Anxiety Disorders
 Atrophy Antibodies  >120 publications with HTT and Atrophy
 Weight Loss Antibodies  >58 publications with HTT and Weight Loss
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1402 publications with HTT and Brain
 Blood Antibodies  >325 publications with HTT and Blood
 Muscle Antibodies  >84 publications with HTT and Muscle
 Embryonic Tissue Antibodies  >81 publications with HTT and Embryonic Tissue
 Vascular Antibodies  >56 publications with HTT and Vascular
 Liver Antibodies  >42 publications with HTT and Liver
 Eye Antibodies  >40 publications with HTT and Eye
 Lung Antibodies  >35 publications with HTT and Lung
 Thyroid Antibodies  >33 publications with HTT and Thyroid
 Kidney Antibodies  >32 publications with HTT and Kidney
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