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HMGCS2 elisa kit :: Human Hydroxymethylglutaryl CoA synthase, mitochondrial (HMGCS2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7222751
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
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 Product Name   

Hydroxymethylglutaryl CoA synthase, mitochondrial (HMGCS2), ELISA Kit

★Popular Item★
 Also Known As   

Human Hydroxymethylglutaryl CoA synthase, mitochondrial (HMGCS2) ELISA Kit

 Product Gene Name   

HMGCS2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 605911
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of HMGCS2. No significant cross-reactivity or interference between HMGCS2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HMGCS2 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HMGCS2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HMGCS2 purchase    MBS7222751 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hydroxymethylglutaryl CoA synthase, mitochondrial (HMGCS2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HMGCS2, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7222751 kit is based on HMGCS2 antibody-HMGCS2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HMGCS2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HMGCS2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HMGCS2 elisa kit

   Intended Uses: This HMGCS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HMGCS2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: HMGCS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HMGCS2 antibody and an HMGCS2-HRP conjugate. The assay sample and buffer are incubated together with HMGCS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMGCS2 concentration since HMGCS2 from samples and HMGCS2-HRP conjugate compete for the anti-HMGCS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HMGCS2 from the sample, fewer sites are left to bind HMGCS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGCS2 concentration in each sample is interpolated from this standard curve.
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 Typical Standard Curve/Testing Data of HMGCS2 elisa kit    HMGCS2 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS7222751. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HMGCS2. It may not necessarily be applicable to this product.
 NCBI GI #    260656028
 NCBI GeneID    3158
 NCBI Accession #    NP_001159579.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001166107.1 [Other Products]
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 UniProt Secondary Accession #    Q5SZU2; Q6IBF4; B7Z8R3; D3Y5K6 [Other Products]
 UniProt Related Accession #    P54868 [Other Products]
 Molecular Weight    50,050 Da
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 NCBI Official Full Name    hydroxymethylglutaryl-CoA synthase, mitochondrial isoform 2
 NCBI Official Synonym Full Names    3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial)
 NCBI Official Symbol    HMGCS2 [Similar Products]
 NCBI Protein Information    hydroxymethylglutaryl-CoA synthase, mitochondrial; HMG-CoA synthase; 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial)
 UniProt Protein Name    Hydroxymethylglutaryl-CoA synthase, mitochondrial
 UniProt Synonym Protein Names   
3-hydroxy-3-methylglutaryl coenzyme A synthase
 UniProt Gene Name    HMGCS2 [Similar Products]
 UniProt Synonym Gene Names    HMG-CoA synthase [Similar Products]
 UniProt Entry Name    HMCS2_HUMAN
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 NCBI Summary for HMGCS2    The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
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 UniProt Comments for HMGCS2    HMGCS2: This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Defects in HMGCS2 are the cause of HMG-CoA synthase deficiency (HMGCS deficiency); also known as deficiency of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2. Affected individuals present with severe hypoketotic hypoglycemia, mild hepatomegaly, or fatty liver, and a nondiagnostic pattern of urinary organic acids with increase of medium and short chain dicarboxylic acids. Belongs to the HMG-CoA synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.3.10; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Mitochondrial; Carbohydrate Metabolism - butanoate; Lipid Metabolism - synthesis and degradation of ketone bodies; Transferase

Chromosomal Location of Human Ortholog: 1p13-p12

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: hydroxymethylglutaryl-CoA synthase activity

Biological Process: isoprenoid biosynthetic process; ketone body biosynthetic process; ketone body metabolic process; cellular lipid metabolic process; cholesterol biosynthetic process

Disease: 3-hydroxy-3-methylglutaryl-coa Synthase 2 Deficiency
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 Research Articles on HMGCS2    1. In Fe-S cluster-deficient muscles, results show a dramatic up-regulation of the ketogenic enzyme HMGCS2 and the secreted protein FGF21 (fibroblast growth factor 21).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HMGCS2 elisa kitPathways associated with HMGCS2 elisa kit
 Reference Product  PubMed Publications
 ACAT1 elisa kit  >5 publications with HMGCS2 and ACAT1
 HMGCL elisa kit  >4 publications with HMGCS2 and HMGCL
 PPARA elisa kit  >4 publications with HMGCS2 and PPARA
 ACAA2 elisa kit  >2 publications with HMGCS2 and ACAA2
 MYC elisa kit  >2 publications with HMGCS2 and MYC
 ACAT2 elisa kit  >1 publications with HMGCS2 and ACAT2
 Products by Pathway  Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Ketone Body Metabolism Pathway antibodies  Ketone Body Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
Diseases associated with HMGCS2 elisa kitOrgans/Tissues associated with HMGCS2 elisa kit
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >18 publications with HMGCS2 and Fatty Liver
 Liver Diseases Antibodies  >3 publications with HMGCS2 and Liver Diseases
 Nervous System Diseases Antibodies  >3 publications with HMGCS2 and Nervous System Diseases
 Breast Neoplasms Antibodies  >3 publications with HMGCS2 and Breast Neoplasms
 Mitochondrial Diseases Antibodies  >3 publications with HMGCS2 and Mitochondrial Diseases
 Prenatal Exposure Delayed Effects Antibodies  >2 publications with HMGCS2 and Prenatal Exposure Delayed Effects
 Kidney Diseases Antibodies  >2 publications with HMGCS2 and Kidney Diseases
 Dyslipidemias Antibodies  >2 publications with HMGCS2 and Dyslipidemias
 Brain Diseases Antibodies  >2 publications with HMGCS2 and Brain Diseases
 Hypertension Antibodies  >2 publications with HMGCS2 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >30 publications with HMGCS2 and Liver
 Muscle Antibodies  >6 publications with HMGCS2 and Muscle
 Heart Antibodies  >3 publications with HMGCS2 and Heart
 Lung Antibodies  >3 publications with HMGCS2 and Lung
 Kidney Antibodies  >3 publications with HMGCS2 and Kidney
 Intestine Antibodies  >3 publications with HMGCS2 and Intestine
 Brain Antibodies  >2 publications with HMGCS2 and Brain
 Testis Antibodies  >2 publications with HMGCS2 and Testis
 Pancreas Antibodies  >1 publications with HMGCS2 and Pancreas
 Mammary Gland Antibodies  >1 publications with HMGCS2 and Mammary Gland
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