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HIF1a elisa kit :: Human Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS452612 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $345 +1 FREE 8GB USB
96-Strip-Wells  /  $455 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,025 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,920 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hypoxia Inducible Factor 1 Alpha (HIF1a), ELISA Kit

★Popular Item★
 Also Known As   

Human Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

 Product Synonym Names    HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
 Product Gene Name   

HIF1a elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    U22431 mRNA
 3D Structure    ModBase 3D Structure for Q16665
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of HIF1a. No significant cross-reactivity or interference between HIF1a and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.055ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HIF1a were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HIF1a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HIF1a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIF1a purchase    MBS452612 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing HIF1a, with an estimated sensitivity of 0.055ng/mL. The ELISA analytical biochemical technique of the MBS452612 kit is based on HIF1a antibody-HIF1a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF1a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF1a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HIF1a elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HIF1a in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HIF1a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HIF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HIF1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HIF1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of HIF1a elisa kit    HIF1a elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS452612. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HIF1a. It may not necessarily be applicable to this product.
 NCBI GI #    340545531
 NCBI GeneID    3091
 NCBI Accession #    NP_001230013.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001243084.1 [Other Products]
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 UniProt Primary Accession #    Q16665 [Other Products]
 UniProt Secondary Accession #    Q53XP6; Q96PT9; Q9UPB1; C0LZJ3 [Other Products]
 UniProt Related Accession #    Q16665 [Other Products]
 Molecular Weight    95,634 Da
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 NCBI Official Full Name    hypoxia-inducible factor 1-alpha isoform 3
 NCBI Official Synonym Full Names    hypoxia inducible factor 1 alpha subunit
 NCBI Official Symbol    HIF1A [Similar Products]
 NCBI Official Synonym Symbols   
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
[Similar Products]
 NCBI Protein Information    hypoxia-inducible factor 1-alpha
 UniProt Protein Name    Hypoxia-inducible factor 1-alpha
 UniProt Synonym Protein Names   
ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8
 Protein Family    Hypoxia-inducible factor
 UniProt Gene Name    HIF1A [Similar Products]
 UniProt Synonym Gene Names    BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78 [Similar Products]
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 NCBI Summary for HIF1a    This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
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 UniProt Comments for HIF1a    Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
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 Research Articles on HIF1a    1. Results reveal a novel regulatory mechanism of HIF1alpha induction in normoxia and suggest that miR-101 mediated proliferation inhibition may through HIF1alpha mediated apoptosis and cell cycle arrest.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIF1a elisa kitPathways associated with HIF1a elisa kit
 Reference Product  PubMed Publications
 VHL elisa kit  >612 publications with HIF1a and VHL
 ARNT elisa kit  >316 publications with HIF1a and ARNT
 EGLN1 elisa kit  >180 publications with HIF1a and EGLN1
 EGLN3 elisa kit  >75 publications with HIF1a and EGLN3
 HIF1AN elisa kit  >69 publications with HIF1a and HIF1AN
 EP300 elisa kit  >39 publications with HIF1a and EP300
 CREBBP elisa kit  >18 publications with HIF1a and CREBBP
 UBC elisa kit  >6 publications with HIF1a and UBC
 HSP90AA1 elisa kit  >4 publications with HIF1a and HSP90AA1
 TCEB1 elisa kit  >2 publications with HIF1a and TCEB1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway antibodies  BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
Diseases associated with HIF1a elisa kitOrgans/Tissues associated with HIF1a elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1787 publications with HIF1a and Carcinoma
 Inflammation Antibodies  >765 publications with HIF1a and Inflammation
 Lung Diseases Antibodies  >606 publications with HIF1a and Lung Diseases
 Breast Neoplasms Antibodies  >582 publications with HIF1a and Breast Neoplasms
 Kidney Diseases Antibodies  >528 publications with HIF1a and Kidney Diseases
 Liver Diseases Antibodies  >504 publications with HIF1a and Liver Diseases
 Necrosis Antibodies  >474 publications with HIF1a and Necrosis
 Neoplasms, Experimental Antibodies  >431 publications with HIF1a and Neoplasms, Experimental
 Heart Diseases Antibodies  >405 publications with HIF1a and Heart Diseases
 Lung Neoplasms Antibodies  >393 publications with HIF1a and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >4089 publications with HIF1a and Vascular
 Blood Antibodies  >2458 publications with HIF1a and Blood
 Brain Antibodies  >959 publications with HIF1a and Brain
 Muscle Antibodies  >933 publications with HIF1a and Muscle
 Kidney Antibodies  >838 publications with HIF1a and Kidney
 Lung Antibodies  >837 publications with HIF1a and Lung
 Liver Antibodies  >783 publications with HIF1a and Liver
 Heart Antibodies  >718 publications with HIF1a and Heart
 Bone Antibodies  >596 publications with HIF1a and Bone
 Prostate Antibodies  >332 publications with HIF1a and Prostate
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