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HIF-1alpha elisa kit :: Human Hypoxia Inducible Factor 1, Alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS018031 HIF-1alpha elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hypoxia Inducible Factor 1, Alpha (HIF-1alpha), ELISA Kit

 Also Known As   

Human Hypoxia Inducible Factor 1, Alpha ELISA Kit

 Product Gene Name   

HIF-1alpha elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 14; NC_000014.8 (62162119..62214977). Location: 14q23.2
 OMIM    603348
 3D Structure    ModBase 3D Structure for Q16665
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Samples    Undiluted original Human body fluids, tissue homogenates, secretions or feces samples.
 Assay Type    Sandwich (Quantitative)
 Detection Range    6.25 pg/ml - 200 pg/ml.
 Sensitivity    1.0 pg/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HIF-1alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIF-1alpha purchase    MBS018031 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hypoxia Inducible Factor 1, Alpha (HIF-1alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml - 200 pg/ml in biological research samples containing HIF-1alpha, with an estimated sensitivity of 1.0 pg/ml. The ELISA analytical biochemical technique of the MBS018031 kit is based on HIF-1alpha antibody-HIF-1alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF-1alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF-1alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HIF-1alpha elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of HIF1A (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS018031. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HIF-1alpha. It may not necessarily be applicable to this product.
 NCBI GI #    4504385
 NCBI GeneID    3091
 NCBI Accession #    NP_001521.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001530.3 [Other Products]
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 UniProt Primary Accession #    Q16665 [Other Products]
 UniProt Secondary Accession #    Q53XP6; Q96PT9; Q9UPB1; C0LZJ3 [Other Products]
 UniProt Related Accession #    Q16665 [Other Products]
 Molecular Weight    92,670 Da
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 NCBI Official Full Name    hypoxia-inducible factor 1-alpha isoform 1
 NCBI Official Synonym Full Names    hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 NCBI Official Symbol    HIF1A [Similar Products]
 NCBI Official Synonym Symbols   
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
[Similar Products]
 NCBI Protein Information    hypoxia-inducible factor 1-alpha; HIF-1-alpha; member of PAS protein 1; ARNT interacting protein; ARNT-interacting protein; member of PAS superfamily 1; hypoxia-inducible factor1alpha; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 UniProt Protein Name    Hypoxia-inducible factor 1-alpha
 UniProt Synonym Protein Names   
ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8
 UniProt Gene Name    HIF1A [Similar Products]
 UniProt Synonym Gene Names    BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78 [Similar Products]
 UniProt Entry Name    HIF1A_HUMAN
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 UniProt Comments for HIF-1alpha    Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIF-1alpha elisa kitPathways associated with HIF-1alpha elisa kit
 Reference Product  PubMed Publications
 VHL elisa kit  >469 publications with HIF-1alpha and VHL
 ARNT elisa kit  >265 publications with HIF-1alpha and ARNT
 EGLN1 elisa kit  >116 publications with HIF-1alpha and EGLN1
 EGLN3 elisa kit  >60 publications with HIF-1alpha and EGLN3
 HIF1AN elisa kit  >55 publications with HIF-1alpha and HIF1AN
 TP53 elisa kit  >29 publications with HIF-1alpha and TP53
 EP300 elisa kit  >21 publications with HIF-1alpha and EP300
 CREBBP elisa kit  >13 publications with HIF-1alpha and CREBBP
 UBC elisa kit  >4 publications with HIF-1alpha and UBC
 HSP90AA1 elisa kit  >3 publications with HIF-1alpha and HSP90AA1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
Diseases associated with HIF-1alpha elisa kitOrgans/Tissues associated with HIF-1alpha elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2859 publications with HIF-1alpha and Neoplasms
 Carcinoma Antibodies  >1194 publications with HIF-1alpha and Carcinoma
 Nervous System Diseases Antibodies  >574 publications with HIF-1alpha and Nervous System Diseases
 Inflammation Antibodies  >430 publications with HIF-1alpha and Inflammation
 Brain Diseases Antibodies  >420 publications with HIF-1alpha and Brain Diseases
 Breast Neoplasms Antibodies  >369 publications with HIF-1alpha and Breast Neoplasms
 Kidney Diseases Antibodies  >368 publications with HIF-1alpha and Kidney Diseases
 Lung Diseases Antibodies  >344 publications with HIF-1alpha and Lung Diseases
 Necrosis Antibodies  >321 publications with HIF-1alpha and Necrosis
 Liver Diseases Antibodies  >314 publications with HIF-1alpha and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >634 publications with HIF-1alpha and Brain
 Muscle Antibodies  >625 publications with HIF-1alpha and Muscle
 Kidney Antibodies  >594 publications with HIF-1alpha and Kidney
 Lung Antibodies  >519 publications with HIF-1alpha and Lung
 Liver Antibodies  >508 publications with HIF-1alpha and Liver
 Bone Antibodies  >356 publications with HIF-1alpha and Bone
 Prostate Antibodies  >249 publications with HIF-1alpha and Prostate
 Embryonic Tissue Antibodies  >247 publications with HIF-1alpha and Embryonic Tissue
 Bone Marrow Antibodies  >160 publications with HIF-1alpha and Bone Marrow
 Lymph Node Antibodies  >148 publications with HIF-1alpha and Lymph Node
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