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IL16 elisa kit :: Human IL-16 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS176490
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $390 +1 FREE 8GB USB
 
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 Product Name   

IL-16 (IL16), ELISA Kit

★Popular Item★
 Also Known As   

Human IL-16 PicoKine ELISA Kit

 Product Synonym Names    Pro-interleukin-16; interleukin 16; FLJ16806; FLJ42735; FLJ44234; HGNC:5980; HsT19289; IL-16; IL16; IL16_HUMAN; Interleukin 16; Interleukin 16 precursor; Interleukin-16; KIAA4048; LCF; Lymphocyte chemoattractant factor; mKIAA4048; Neuronal interleukin 16; NIL16; OTTHUMP00000185571; OTTHUMP00000190849; OTTHUMP00000190850; PrIL16; Pro interleukin 16; Prointerleukin 16
 Product Gene Name   

IL16 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603035
 3D Structure    ModBase 3D Structure for Q14005
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 Species Reactivity    Human
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 Specificity    Natural and recombinant human IL-16
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 Samples    Cell culture supernates, serum and plasma (heparin, EDTA)
 Assay Type    Sandwich
 Detection Range    31.2pg/ml-2000pg/ml
 Sensitivity    < 1pg/ml
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL16 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL16 purchase    MBS176490 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-16 (IL16) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2pg/ml-2000pg/ml in biological research samples containing IL16, with an estimated sensitivity of < 1pg/ml. The ELISA analytical biochemical technique of the MBS176490 kit is based on IL16 antibody-IL16 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL16 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL16. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL16 elisa kit

   Principle of the assay: human IL-16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-16 has been precoated onto 96-well plates. Standards(E.coli, P2-S130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-16 amount of sample captured in plate.
Background: Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.
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 Typical Testing Data/Standard Curve (for reference only) of IL16 elisa kit    IL16 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS176490. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL16. It may not necessarily be applicable to this product.
 NCBI GI #    239938922
 NCBI GeneID    3603
 NCBI Accession #    Q14005.4 [Other Products]
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 UniProt Primary Accession #    Q14005 [Other Products]
 UniProt Secondary Accession #    Q16435; Q6VVE6; Q6ZMQ7; Q9UP18; A6NM20; A8MU65; B9EGR6 [Other Products]
 UniProt Related Accession #    Q14005 [Other Products]
 Molecular Weight    66,646 Da
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 NCBI Official Full Name    Pro-interleukin-16
 NCBI Official Synonym Full Names    interleukin 16
 NCBI Official Symbol    IL16 [Similar Products]
 NCBI Official Synonym Symbols   
LCF; NIL16; PRIL16; prIL-16
[Similar Products]
 NCBI Protein Information    pro-interleukin-16; prointerleukin 16; neuronal interleukin 16; lymphocyte chemoattractant factor
 UniProt Protein Name    Pro-interleukin-16
 UniProt Synonym Protein Names   
Lymphocyte chemoattractant factor; LCF
 Protein Family    Pro-interleukin
 UniProt Gene Name    IL16 [Similar Products]
 UniProt Synonym Gene Names    IL-16; LCF [Similar Products]
 UniProt Entry Name    IL16_HUMAN
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 NCBI Summary for IL16    The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
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 UniProt Comments for IL16    IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus

Molecular Function: cytokine activity

Biological Process: viral reproduction; regulation of transcription, DNA-dependent; transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis
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 Research Articles on IL16    1. The IL-16 SNP rs8028364 was found to be associated with Graves' disease(GD) when compared with the control subjects.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL16 elisa kitDiseases associated with IL16 elisa kit
 Reference Product  PubMed Publications
 CD4 elisa kit  >220 publications with IL16 and CD4
 CASP3 elisa kit  >30 publications with IL16 and CASP3
 CCR5 elisa kit  >15 publications with IL16 and CCR5
 IL2RA elisa kit  >8 publications with IL16 and IL2RA
 IL2 elisa kit  >5 publications with IL16 and IL2
 FOS elisa kit  >3 publications with IL16 and FOS
 MAP2K4 elisa kit  >1 publications with IL16 and MAP2K4
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >103 publications with IL16 and Inflammation
 Lung Diseases Antibodies  >58 publications with IL16 and Lung Diseases
 Necrosis Antibodies  >52 publications with IL16 and Necrosis
 Intestinal Diseases Antibodies  >25 publications with IL16 and Intestinal Diseases
 Hematologic Diseases Antibodies  >11 publications with IL16 and Hematologic Diseases
 Fibrosis Antibodies  >8 publications with IL16 and Fibrosis
 Liver Diseases Antibodies  >7 publications with IL16 and Liver Diseases
 Kidney Diseases Antibodies  >6 publications with IL16 and Kidney Diseases
 Breast Neoplasms Antibodies  >6 publications with IL16 and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >6 publications with IL16 and Prostatic Neoplasms
Organs/Tissues associated with IL16 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >349 publications with IL16 and Blood
 Lung Antibodies  >56 publications with IL16 and Lung
 Skin Antibodies  >32 publications with IL16 and Skin
 Brain Antibodies  >27 publications with IL16 and Brain
 Lymph Node Antibodies  >17 publications with IL16 and Lymph Node
 Lymph Antibodies  >17 publications with IL16 and Lymph
 Liver Antibodies  >14 publications with IL16 and Liver
 Intestine Antibodies  >12 publications with IL16 and Intestine
 Bone Marrow Antibodies  >10 publications with IL16 and Bone Marrow
 Heart Antibodies  >9 publications with IL16 and Heart
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