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IL-17 elisa kit :: Human IL-17 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS355268
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $440 +1 FREE 8GB USB
 
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 Product Name   

IL-17, ELISA Kit

★Popular Item★
 Also Known As   

IL-17 Human ELISA Kit

 Product Synonym Names    Human IL-17
 Product Gene Name   

IL-17 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS355268 MSDS    MSDS PDF
 MBS355268 Technical Datasheet    Technical Datasheet PDF
 Species Reactivity    Human
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 Samples    human serum, plasma, body fluids, tissue lysates or cell culture supernatants
 Detection Range    15.6 pg/ml-1000 pg/ml
 Sensitivity    < 1 pg/ml
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 Preparation and Storage    Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-17 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-17 purchase    MBS355268 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-17, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml-1000 pg/ml in biological research samples containing IL-17, with an estimated sensitivity of < 1 pg/ml. The ELISA analytical biochemical technique of the MBS355268 kit is based on IL-17 antibody-IL-17 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-17 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-17. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as human serum, plasma, body fluids, tissue lysates or cell culture supernatants. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-17 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-17 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-17 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-17 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-17 can be calculated.

Background: Interleukin-17(IL-17 or IL-17A) originally identified as a transcript from a rodent T-cell hybridoma by Rouvier et al, in 1993. The IL-7 gene maps to human chromosome 2q31. It is the founding member of a group of cytokines called the IL-17 family, and secreted primarily by activated T cells. IL-17 shows high homology to viral IL-17 encoded by an open reading frame of the T-lymphotropic rhadinovirus Herpesvirus saimiri. All members of the IL-17 family have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, yet they have no sequence similarity to any other known cytokines. The most notable role of IL-17 is it involvement in inducing and mediating proinflammatory responses. IL-17F has been well characterized both in vitro and in vivo, and has been shown to have a pro-inflammatory role in asthma.
 Applications Tested/Suitable for IL-17 elisa kit   

Quantitative sELISA (EIA)

 Application Notes for IL-17 elisa kit    For quantitative detection of IL-17 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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 Typical Testing Data/Standard Curve (for reference only) of IL-17 elisa kit    IL-17 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS355268. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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