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IL-32 elisa kit :: Human IL-32 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2503175
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
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 Product Name   

IL-32, ELISA Kit

★Popular Item★
 Also Known As   

Human IL-32(Interleukin 32)ELISA Kit

 Product Gene Name   

IL-32 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606001
 3D Structure    ModBase 3D Structure for P24001
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human IL-32. No significant cross-reactivity or interference between Human IL-32 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human IL-32 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    Min: 9.375pg/mL; Max: 1000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-32 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-32 purchase    MBS2503175 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-32, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing IL-32, with an estimated sensitivity of Min: 9.375pg/mL; Max: 1000pg/mL. The ELISA analytical biochemical technique of the MBS2503175 kit is based on IL-32 antibody-IL-32 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-32 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-32. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-32 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL-32 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-32. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human IL-32 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-32, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IL-32. You can calculate the concentration of Human IL-32 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IL-32 elisa kit    IL-32 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2503175. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-32. It may not necessarily be applicable to this product.
 NCBI GI #    61658638
 NCBI GeneID    9235
 NCBI Accession #    NP_001012653.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001012635.1 [Other Products]
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 UniProt Primary Accession #    P24001 [Other Products]
 UniProt Secondary Accession #    Q5VFH7; Q5VFH8; Q8WV38; Q96GK9; A6NNM0; A8MPX0; B4DJM1; B8Q191; D3DUB0; D3DUB2 [Other Products]
 UniProt Related Accession #    P24001 [Other Products]
 Molecular Weight    17,003 Da
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 NCBI Official Full Name    interleukin-32 isoform C
 NCBI Official Synonym Full Names    interleukin 32
 NCBI Official Symbol    IL32 [Similar Products]
 NCBI Official Synonym Symbols   
NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
[Similar Products]
 NCBI Protein Information    interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cell transcript 4; natural killer cells protein 4; tumor necrosis factor alpha-inducing factor
 UniProt Protein Name    Interleukin-32
 UniProt Synonym Protein Names   
Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor
 UniProt Gene Name    IL32 [Similar Products]
 UniProt Synonym Gene Names    NK4; TAIF; IL-32 [Similar Products]
 UniProt Entry Name    IL32_HUMAN
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 NCBI Summary for IL-32    This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL-32    IL32: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF- alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular space; membrane

Molecular Function: protein binding; cytokine activity

Biological Process: defense response; immune response; cell adhesion
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 Research Articles on IL-32    1. IL-32alpha-mediated STAT3 S727 phosphorylation induced C/EBPalpha association, which inhibited PU.1 expression, and then resulted in the down-regulation of CD18 expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-32 elisa kitDiseases associated with IL-32 elisa kit
 Reference Product  PubMed Publications
 IL18 elisa kit  >5 publications with IL-32 and IL18
 PTGS2 elisa kit  >2 publications with IL-32 and PTGS2
 IL1B elisa kit  >2 publications with IL-32 and IL1B
 CXCL2 elisa kit  >1 publications with IL-32 and CXCL2
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >65 publications with IL-32 and Inflammation
 Necrosis Antibodies  >53 publications with IL-32 and Necrosis
 Immune System Diseases Antibodies  >34 publications with IL-32 and Immune System Diseases
 Lung Diseases Antibodies  >14 publications with IL-32 and Lung Diseases
 Vascular Diseases Antibodies  >12 publications with IL-32 and Vascular Diseases
 Carcinoma Antibodies  >11 publications with IL-32 and Carcinoma
 Nervous System Diseases Antibodies  >9 publications with IL-32 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >9 publications with IL-32 and Cardiovascular Diseases
 Liver Diseases Antibodies  >7 publications with IL-32 and Liver Diseases
 Lung Neoplasms Antibodies  >6 publications with IL-32 and Lung Neoplasms
Organs/Tissues associated with IL-32 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >64 publications with IL-32 and Blood
 Lung Antibodies  >18 publications with IL-32 and Lung
 Vascular Antibodies  >15 publications with IL-32 and Vascular
 Bone Antibodies  >10 publications with IL-32 and Bone
 Intestine Antibodies  >9 publications with IL-32 and Intestine
 Liver Antibodies  >8 publications with IL-32 and Liver
 Bone Marrow Antibodies  >7 publications with IL-32 and Bone Marrow
 Stomach Antibodies  >6 publications with IL-32 and Stomach
 Lymph Node Antibodies  >6 publications with IL-32 and Lymph Node
 Brain Antibodies  >4 publications with IL-32 and Brain
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