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IL6R elisa kit :: Human IL-6R alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS176529
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IL-6R alpha (IL6R), ELISA Kit

 Also Known As   

Human IL-6R alpha PicoKine ELISA Kit

 Product Synonym Names    Interleukin-6 receptor subunit alpha; interleukin 6 receptor; CD126; CD126 antigen; Gp80; IL 6R 1; IL 6R alpha; IL-6 receptor subunit alpha; IL-6R 1; IL-6R subunit alpha; IL-6R-1; IL-6R-alpha; IL-6RA; Il6r; IL6R1; IL6RA; IL6RA_HUMAN; IL6Ralpha; Interleukin 6 receptor; Interleukin 6 receptor alpha chain; Interleukin-6 receptor subunit alpha; Membrane glycoprotein 80; MGC104991; OTTHUMP00000034320
 Product Gene Name   

IL6R elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for A0N0L5
 Species Reactivity    Human
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 Specificity    Natural and recombinant human IL-6R Alpha
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 Samples    Cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine
 Assay Type    Sandwich
 Detection Range    31.2pg/ml-2000pg/ml
 Sensitivity    < 10pg/ml
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL6R elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL6R purchase    MBS176529 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-6R alpha (IL6R) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2pg/ml-2000pg/ml in biological research samples containing IL6R, with an estimated sensitivity of < 10pg/ml. The ELISA analytical biochemical technique of the MBS176529 kit is based on IL6R antibody-IL6R antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL6R antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL6R. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL6R elisa kit

   Principle of the assay: human IL-6R Alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6R Alpha has been precoated onto 96-well plates. Standards(sf21,L20-D358) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6R Alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-6R Alpha amount of sample captured in plate.
Background: IL6R alpha(IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.
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 Typical Testing Data/Standard Curve (for reference only) of IL6R elisa kit    Human IL-6R alpha PicoKine ELISA Kit standard curve
IL6R elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS176529. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL6R. It may not necessarily be applicable to this product.
 NCBI GeneID    3570
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 UniProt Primary Accession #    A0N0L5 [Other Products]
 UniProt Related Accession #    P08887 [Other Products]
 Molecular Weight    51,548 Da
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 NCBI Official Synonym Full Names    interleukin 6 receptor
 NCBI Official Symbol    IL6R [Similar Products]
 NCBI Official Synonym Symbols   
IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
[Similar Products]
 NCBI Protein Information    interleukin-6 receptor subunit alpha; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha
 UniProt Protein Name    Interleukin 6 receptor
 UniProt Synonym Protein Names   
Interleukin 6 receptor
 Protein Family    Interleukin-6 receptor
 UniProt Gene Name    IL6R [Similar Products]
 UniProt Entry Name    A0N0L5_HUMAN
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 NCBI Summary for IL6R    This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.[provided by RefSeq, May 2011]
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 UniProt Comments for IL6R    IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; basolateral plasma membrane; apical plasma membrane; plasma membrane; extracellular region; interleukin-6 receptor complex

Molecular Function: protein binding; protein homodimerization activity; interleukin-6 receptor binding; interleukin-6 binding; enzyme binding; ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of collagen biosynthetic process; neutrophil mediated immunity; positive regulation of interleukin-6 production; positive regulation of leukocyte chemotaxis; defense response to Gram-negative bacterium; endocrine pancreas development; positive regulation of chemokine production; positive regulation of tyrosine phosphorylation of Stat3 protein; activation of NF-kappaB transcription factor; monocyte chemotaxis; positive regulation of osteoblast differentiation; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; positive regulation of MAPKKK cascade; response to cytokine stimulus; positive regulation of cell proliferation; acute-phase response; negative regulation of interleukin-8 production; hepatic immune response

Disease: Interleukin 6, Serum Level Of, Quantitative Trait Locus; Soluble Interleukin-6 Receptor, Serum Level Of, Quantitative Trait Locus
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 Research Articles on IL6R    1. R28E mutation in CNTF abrogatesIL-6 receptor-dependent but retains CNTF receptor-dependent signaling via glycoprotein 130/ LIFR.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL6R elisa kitDiseases associated with IL6R elisa kit
 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 IL-6 Signaling Pathway antibodies  IL-6 Signaling Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interleukin-6 Signaling Pathway antibodies  Interleukin-6 Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >85 publications with IL6R and Inflammation
 Immune System Diseases Antibodies  >51 publications with IL6R and Immune System Diseases
 Cardiovascular Diseases Antibodies  >44 publications with IL6R and Cardiovascular Diseases
 Arthritis, Rheumatoid Antibodies  >31 publications with IL6R and Arthritis, Rheumatoid
 Necrosis Antibodies  >27 publications with IL6R and Necrosis
 Heart Diseases Antibodies  >24 publications with IL6R and Heart Diseases
 Nervous System Diseases Antibodies  >13 publications with IL6R and Nervous System Diseases
 Carcinoma, Hepatocellular Antibodies  >10 publications with IL6R and Carcinoma, Hepatocellular
 Brain Diseases Antibodies  >9 publications with IL6R and Brain Diseases
 Liver Diseases Antibodies  >9 publications with IL6R and Liver Diseases
Organs/Tissues associated with IL6R elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >105 publications with IL6R and Blood
 Heart Antibodies  >31 publications with IL6R and Heart
 Vascular Antibodies  >22 publications with IL6R and Vascular
 Liver Antibodies  >16 publications with IL6R and Liver
 Lung Antibodies  >12 publications with IL6R and Lung
 Bone Antibodies  >11 publications with IL6R and Bone
 Kidney Antibodies  >10 publications with IL6R and Kidney
 Muscle Antibodies  >9 publications with IL6R and Muscle
 Brain Antibodies  >9 publications with IL6R and Brain
 Prostate Antibodies  >4 publications with IL6R and Prostate
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