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ILP2 elisa kit :: Human Inhibitor of apoptosis protein Like Protein2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS722176
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Inhibitor of apoptosis protein Like Protein2 (ILP2), ELISA Kit

 Also Known As   

Human Inhibitor of apoptosis protein Like Protein2 ELISA Kit

 Product Gene Name   

ILP2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IPLP-2. No significant cross-reactivity or interference between IPLP-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IPLP-2 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
 Intended Uses    This IPLP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IPLP-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ILP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ILP2 purchase    MBS722176 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Inhibitor of apoptosis protein Like Protein2 (ILP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ILP2, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS722176 kit is based on ILP2 antibody-ILP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ILP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ILP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ILP2 elisa kit

   Priciple of the assay: IPLP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IPLP-2 antibody and an IPLP-2-HRP conjugate. The assay sample and buffer are incubated together with IPLP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IPLP-2 concentration since IPLP-2 from samples and IPLP-2-HRP conjugate compete for the anti-IPLP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IPLP-2 from the sample, fewer sites are left to bind IPLP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IPLP-2 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ILP2 elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of ILP2 elisa kit    ILP2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS722176. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ILP2. It may not necessarily be applicable to this product.
 NCBI GI #    15042064
 NCBI GeneID    112401
 NCBI Accession #    AAK81892.1 [Other Products]
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 UniProt Secondary Accession #    Q6IPY1; Q96RW5 [Other Products]
 UniProt Related Accession #    Q96P09 [Other Products]
 Molecular Weight    27,089 Da
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 NCBI Official Full Name    IAP-like protein 2
 NCBI Official Synonym Full Names    baculoviral IAP repeat containing 8
 NCBI Official Symbol    BIRC8 [Similar Products]
 NCBI Official Synonym Symbols   
ILP2; ILP-2; hILP2
[Similar Products]
 NCBI Protein Information    baculoviral IAP repeat-containing protein 8; IAP-like protein 2; baculoviral IAP repeat-containing 8; inhibitor of apoptosis-like protein 2; testis-specific inhibitor of apoptosis
 UniProt Protein Name    Baculoviral IAP repeat-containing protein 8
 UniProt Synonym Protein Names   
Inhibitor of apoptosis-like protein 2; IAP-like protein 2; ILP-2; Testis-specific inhibitor of apoptosis
 Protein Family    Probable insulin-like peptide
 UniProt Gene Name    BIRC8 [Similar Products]
 UniProt Synonym Gene Names    ILP2; IAP-like protein 2; ILP-2 [Similar Products]
 UniProt Entry Name    BIRC8_HUMAN
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 UniProt Comments for ILP2    BIRC8: Protects against apoptosis mediated by BAX. Belongs to the IAP family.

Protein type: Apoptosis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: -

Cellular Component: spindle microtubule; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protease binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity

Biological Process: neuron apoptosis; protein ubiquitination; negative regulation of neuron apoptosis; negative regulation of apoptosis
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 Research Articles on ILP2    1. increased expression with progression of chronic myeloid leukaemia
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ILP2 elisa kitPathways associated with ILP2 elisa kit
 Reference Product  PubMed Publications
 CASP9 elisa kit  >4 publications with ILP2 and CASP9
 BIRC5 elisa kit  >3 publications with ILP2 and BIRC5
 APAF1 elisa kit  >2 publications with ILP2 and APAF1
 DIABLO elisa kit  >1 publications with ILP2 and DIABLO
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Small Cell Lung Cancer Pathway antibodies  Small Cell Lung Cancer Pathway Diagram
 Small Cell Lung Cancer Pathway antibodies  Small Cell Lung Cancer Pathway Diagram
 Toxoplasmosis Pathway antibodies  Toxoplasmosis Pathway Diagram
 Toxoplasmosis Pathway antibodies  Toxoplasmosis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
Diseases associated with ILP2 elisa kitOrgans/Tissues associated with ILP2 elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >2 publications with ILP2 and Kidney Diseases
 Necrosis Antibodies  >1 publications with ILP2 and Necrosis
 Nervous System Diseases Antibodies  >1 publications with ILP2 and Nervous System Diseases
 Disease Models, Animal Antibodies  >1 publications with ILP2 and Disease Models, Animal
 Acute Kidney Injury Antibodies  >1 publications with ILP2 and Acute Kidney Injury
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >4 publications with ILP2 and Testis
 Brain Antibodies  >1 publications with ILP2 and Brain
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