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IRS-1 elisa kit :: Human Insulin Receptor Substrate 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS024501 IRS-1 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Insulin Receptor Substrate 1 (IRS-1), ELISA Kit

 Also Known As   

Human Insulin Receptor Substrate 1 ELISA Kit

 Product Gene Name   

IRS-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 2; NC_000002.11 (227596033..227663506, complement). Location: 2q36
 OMIM    125853
 3D Structure    ModBase 3D Structure for P35568
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between Human IRS-1 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    15.6-500pg/ml.
 Sensitivity    2.0pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IRS-1 concentrations in Human serum, plasma and other body fluids. Using Purified Human IRS-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IRS-1 and IRS-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IRS-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IRS-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IRS-1 purchase    MBS024501 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Insulin Receptor Substrate 1 (IRS-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6-500pg/ml in biological research samples containing IRS-1, with an estimated sensitivity of 2.0pg/ml. The ELISA analytical biochemical technique of the MBS024501 kit is based on IRS-1 antibody-IRS-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IRS-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IRS-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IRS-1 elisa kit

   Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IRS-1 concentrations in Human serum, plasma and other body fluids. Using Purified Human IRS-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IRS-1 and IRS-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IRS-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS024501. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IRS-1. It may not necessarily be applicable to this product.
 NCBI GI #    5031805
 NCBI GeneID    3667
 NCBI Accession #    NP_005535.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005544.2 [Other Products]
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 UniProt Primary Accession #    P35568 [Other Products]
 UniProt Related Accession #    P35568 [Other Products]
 Molecular Weight    131,591 Da
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 NCBI Official Full Name    insulin receptor substrate 1
 NCBI Official Synonym Full Names    insulin receptor substrate 1
 NCBI Official Symbol    IRS1 [Similar Products]
 NCBI Official Synonym Symbols   
HIRS-1
[Similar Products]
 NCBI Protein Information    insulin receptor substrate 1; IRS-1
 UniProt Protein Name    Insulin receptor substrate 1
 Protein Family    Insulin receptor substrate
 UniProt Gene Name    IRS1 [Similar Products]
 UniProt Synonym Gene Names    IRS-1 [Similar Products]
 UniProt Entry Name    IRS1_HUMAN
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 NCBI Summary for IRS-1    This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
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 UniProt Comments for IRS-1    IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; nucleus; cytosol

Molecular Function: insulin-like growth factor receptor binding; signal transducer activity; protein binding; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; signal transduction; glucose homeostasis; response to insulin stimulus; negative regulation of insulin secretion; positive regulation of glucose import; mammary gland development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent
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 Research Articles on IRS-1    1. ectopic expression of miR-96 caused a substantial decrease in IRS-1 protein expression, and a consequent impairment in insulin signaling
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IRS-1 elisa kitPathways associated with IRS-1 elisa kit
 Reference Product  PubMed Publications
 IGF1R elisa kit  >222 publications with IRS-1 and IGF1R
 GRB2 elisa kit  >137 publications with IRS-1 and GRB2
 JAK2 elisa kit  >58 publications with IRS-1 and JAK2
 PTPN11 elisa kit  >41 publications with IRS-1 and PTPN11
 INSR elisa kit  >38 publications with IRS-1 and INSR
 JAK1 elisa kit  >25 publications with IRS-1 and JAK1
 MAPK8 elisa kit  >22 publications with IRS-1 and MAPK8
 PIK3R1 elisa kit  >11 publications with IRS-1 and PIK3R1
 PIK3CA elisa kit  >5 publications with IRS-1 and PIK3CA
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
Diseases associated with IRS-1 elisa kitOrgans/Tissues associated with IRS-1 elisa kit
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >1117 publications with IRS-1 and Insulin Resistance
 Hyperinsulinism Antibodies  >757 publications with IRS-1 and Hyperinsulinism
 Diabetes Mellitus Antibodies  >450 publications with IRS-1 and Diabetes Mellitus
 Neoplasms Antibodies  >355 publications with IRS-1 and Neoplasms
 Diabetes Mellitus, Type 2 Antibodies  >315 publications with IRS-1 and Diabetes Mellitus, Type 2
 Necrosis Antibodies  >161 publications with IRS-1 and Necrosis
 Hyperglycemia Antibodies  >158 publications with IRS-1 and Hyperglycemia
 Liver Diseases Antibodies  >118 publications with IRS-1 and Liver Diseases
 Inflammation Antibodies  >112 publications with IRS-1 and Inflammation
 Cardiovascular Diseases Antibodies  >99 publications with IRS-1 and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >800 publications with IRS-1 and Muscle
 Liver Antibodies  >441 publications with IRS-1 and Liver
 Connective Tissue Antibodies  >224 publications with IRS-1 and Connective Tissue
 Pancreas Antibodies  >135 publications with IRS-1 and Pancreas
 Heart Antibodies  >110 publications with IRS-1 and Heart
 Brain Antibodies  >106 publications with IRS-1 and Brain
 Ovary Antibodies  >105 publications with IRS-1 and Ovary
 Kidney Antibodies  >90 publications with IRS-1 and Kidney
 Nerve Antibodies  >43 publications with IRS-1 and Nerve
 Bone Antibodies  >39 publications with IRS-1 and Bone
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