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ITGb2 elisa kit :: Human Integrin Beta 2 (ITGb2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019445 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $355 +1 FREE 8GB USB
48-Strip-Wells  /  $540 +1 FREE 8GB USB
96-Strip-Wells  /  $730 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,985 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,410 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Integrin Beta 2 (ITGb2), ELISA Kit

 Also Known As   

Integrin Beta 2 (ITGb2) ELISA Kit

 Product Synonym Names    CD18; ITG-B2; LAD; LFA1; MAC1; LCAMB; MF17; LFAb; Complement Component 3 Receptor 3 And 4 Subunit; Lymphocyte Function-Associated Antigen 1; Macrophage Antigen 1
 Product Gene Name   

ITGb2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    116920
 3D Structure    ModBase 3D Structure for P05107
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Integrin Beta 2 (ITGb2).
No significant cross-reactivity or interference between Integrin Beta 2 (ITGb2) and analogues was observed.
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 Samples    Tissue homogenates, Cell lysates, cell culture supernates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    Typically less than 0.129ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Beta 2 (ITGb2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Beta 2 (ITGb2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ITGb2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ITGb2 purchase    MBS2019445 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Integrin Beta 2 (ITGb2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing ITGb2, with an estimated sensitivity of Typically less than 0.129ng/mL. The ELISA analytical biochemical technique of the MBS2019445 kit is based on ITGb2 antibody-ITGb2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ITGb2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ITGb2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ITGb2 elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 2 (ITGb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 2 (ITGb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 2 (ITGb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Beta 2 (ITGb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ITGb2 elisa kit    ITGb2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019445. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ITGb2. It may not necessarily be applicable to this product.
 NCBI GI #    89191865
 NCBI GeneID    3689
 NCBI Accession #    NP_000202.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000211.3 [Other Products]
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 UniProt Primary Accession #    P05107 [Other Products]
 UniProt Secondary Accession #    Q16418; Q53HS5; Q9UD72; B3KTS8; D3DSM1 [Other Products]
 UniProt Related Accession #    P05107 [Other Products]
 Molecular Weight    84,782 Da
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 NCBI Official Full Name    integrin beta-2
 NCBI Official Synonym Full Names    integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
 NCBI Official Symbol    ITGB2 [Similar Products]
 NCBI Official Synonym Symbols   
LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
[Similar Products]
 NCBI Protein Information    integrin beta-2
 UniProt Protein Name    Integrin beta-2
 UniProt Synonym Protein Names   
Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD_antigen: CD18
 Protein Family    Integrin
 UniProt Gene Name    ITGB2 [Similar Products]
 UniProt Synonym Gene Names    CD18; MFI7 [Similar Products]
 UniProt Entry Name    ITB2_HUMAN
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 NCBI Summary for ITGb2    This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
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 UniProt Comments for ITGb2    ITGB2: the integrin beta 2 subunit. Can combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Participates in cell adhesion as well as cell-surface mediated signaling. Defects are the cause of leukocyte adhesion deficiency type I (LAD1).

Protein type: Receptor, misc.; Cell surface; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cell surface; membrane; plasma membrane; integrin complex; receptor complex; vesicle; lipid raft

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; ICAM-3 receptor activity; cell adhesion molecule binding; glycoprotein binding; protein kinase binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; apoptosis; cell-matrix adhesion; natural killer cell activation; receptor clustering; activation of NF-kappaB transcription factor; regulation of cell shape; leukocyte adhesion; cellular extravasation; cell-cell signaling; inflammatory response; cell adhesion; regulation of peptidyl-tyrosine phosphorylation; toll-like receptor 4 signaling pathway; aging; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; activated T cell proliferation; leukocyte migration during inflammatory response; heterotypic cell-cell adhesion; positive regulation of angiogenesis; toll-like receptor signaling pathway; endothelial cell migration; innate immune response; blood coagulation; leukocyte migration

Disease: Leukocyte Adhesion Deficiency, Type I
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 Research Articles on ITGb2    1. the blocking of LFA-1/ICAM-1 interaction resulted in a significant increase in the percentage of IL-17(+) T cells expanded in nonclassical monocyte/T-cell cocultures
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ITGb2 elisa kitPathways associated with ITGb2 elisa kit
 Reference Product  PubMed Publications
 ITGAM elisa kit  >1646 publications with ITGb2 and ITGAM
 ITGAL elisa kit  >1095 publications with ITGb2 and ITGAL
 ICAM1 elisa kit  >880 publications with ITGb2 and ICAM1
 ITGAX elisa kit  >410 publications with ITGb2 and ITGAX
 ICAM2 elisa kit  >58 publications with ITGb2 and ICAM2
 ICAM3 elisa kit  >48 publications with ITGb2 and ICAM3
 ITGAD elisa kit  >18 publications with ITGb2 and ITGAD
 JAM3 elisa kit  >6 publications with ITGb2 and JAM3
 ITGA7 elisa kit  >1 publications with ITGb2 and ITGA7
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
Diseases associated with ITGb2 elisa kitOrgans/Tissues associated with ITGb2 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >926 publications with ITGb2 and Inflammation
 Necrosis Antibodies  >618 publications with ITGb2 and Necrosis
 Cardiovascular Diseases Antibodies  >406 publications with ITGb2 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >235 publications with ITGb2 and Disease Models, Animal
 Lung Diseases Antibodies  >221 publications with ITGb2 and Lung Diseases
 Leukocyte-Adhesion Deficiency Syndrome Antibodies  >166 publications with ITGb2 and Leukocyte-Adhesion Deficiency Syndrome
 Heart Diseases Antibodies  >130 publications with ITGb2 and Heart Diseases
 Leukocyte Disorders Antibodies  >114 publications with ITGb2 and Leukocyte Disorders
 Hypersensitivity Antibodies  >113 publications with ITGb2 and Hypersensitivity
 Atherosclerosis Antibodies  >74 publications with ITGb2 and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3735 publications with ITGb2 and Blood
 Lung Antibodies  >375 publications with ITGb2 and Lung
 Bone Antibodies  >294 publications with ITGb2 and Bone
 Bone Marrow Antibodies  >264 publications with ITGb2 and Bone Marrow
 Heart Antibodies  >187 publications with ITGb2 and Heart
 Muscle Antibodies  >163 publications with ITGb2 and Muscle
 Liver Antibodies  >128 publications with ITGb2 and Liver
 Embryonic Tissue Antibodies  >123 publications with ITGb2 and Embryonic Tissue
 Brain Antibodies  >104 publications with ITGb2 and Brain
 Spleen Antibodies  >104 publications with ITGb2 and Spleen
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