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ISG20 elisa kit :: Human Interferon stimulated gene 20 kDa protein (ISG20) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7235984
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interferon stimulated gene 20 kDa protein (ISG20), ELISA Kit

★Popular Item★
 Also Known As   

Human Interferon stimulated gene 20 kDa protein (ISG20) ELISA Kit

 Product Gene Name   

ISG20 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    604533
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ISG20. No significant cross-reactivity or interference between ISG20 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ISG20 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ISG20 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ISG20 purchase    MBS7235984 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon stimulated gene 20 kDa protein (ISG20) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ISG20, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7235984 kit is based on ISG20 antibody-ISG20 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ISG20 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ISG20. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ISG20 elisa kit

   Intended Uses: This ISG20 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ISG20. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ISG20 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ISG20 antibody and an ISG20-HRP conjugate. The assay sample and buffer are incubated together with ISG20-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ISG20 concentration since ISG20 from samples and ISG20-HRP conjugate compete for the anti-ISG20 antibody binding site. Since the number of sites is limited, as more sites are occupied by ISG20 from the sample, fewer sites are left to bind ISG20-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ISG20 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ISG20 elisa kit    Immunology
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  Typical Testing Data/Standard Curve (for reference only) of ISG20 elisa kit    ISG20 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7235984. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ISG20. It may not necessarily be applicable to this product.
 NCBI GI #    6857800
 NCBI GeneID    3669
 NCBI Accession #    NP_002192.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002201.4 [Other Products]
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 UniProt Secondary Accession #    O00441; O00586 [Other Products]
 UniProt Related Accession #    Q96AZ6 [Other Products]
 Molecular Weight    9,199 Da
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 NCBI Official Full Name    interferon-stimulated gene 20 kDa protein
 NCBI Official Synonym Full Names    interferon stimulated exonuclease gene 20kDa
 NCBI Official Symbol    ISG20 [Similar Products]
 NCBI Official Synonym Symbols   
CD25; HEM45
[Similar Products]
 NCBI Protein Information    interferon-stimulated gene 20 kDa protein; estrogen-regulated transcript 45 protein; promyelocytic leukemia nuclear body-associated protein ISG20
 UniProt Protein Name    Interferon-stimulated gene 20 kDa protein
 UniProt Synonym Protein Names   
Estrogen-regulated transcript 45 protein; Promyelocytic leukemia nuclear body-associated protein ISG20
 Protein Family    Interferon-stimulated gene 20 kDa protein
 UniProt Gene Name    ISG20 [Similar Products]
 UniProt Synonym Gene Names    HEM45 [Similar Products]
 UniProt Entry Name    ISG20_HUMAN
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 UniProt Comments for ISG20    ISG20: Exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses. Belongs to the exonuclease superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Deoxyribonuclease; Ribonuclease; EC 3.1.13.1; Nucleolus

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: nucleoplasm; Cajal body; PML body; cytoplasm; nucleolus; nucleus

Molecular Function: exoribonuclease II activity; 3'-5'-exoribonuclease activity; U1 snRNA binding; metal ion binding; exonuclease activity; single-stranded DNA specific 3'-5' exodeoxyribonuclease activity; U2 snRNA binding

Biological Process: cell proliferation; negative regulation of viral genome replication; response to virus; cytokine and chemokine mediated signaling pathway; RNA catabolic process; DNA catabolic process, exonucleolytic; defense response to virus; rRNA processing
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 Research Articles on ISG20    1. The results suggest that hantavirus infection interferes with DAXX-mediated apoptosis, and expression of interferon-activated Sp100 and ISG-20 proteins may indicate intracellular intrinsic antiviral attempts.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ISG20 elisa kitPathways associated with ISG20 elisa kit
 Reference Product  PubMed Publications
 ISG15 elisa kit  >6 publications with ISG20 and ISG15
 IRF7 elisa kit  >4 publications with ISG20 and IRF7
 IFIT1 elisa kit  >3 publications with ISG20 and IFIT1
 MX1 elisa kit  >3 publications with ISG20 and MX1
 IFIT2 elisa kit  >3 publications with ISG20 and IFIT2
 OAS1 elisa kit  >2 publications with ISG20 and OAS1
 STAT2 elisa kit  >2 publications with ISG20 and STAT2
 IFIT3 elisa kit  >1 publications with ISG20 and IFIT3
 OASL elisa kit  >1 publications with ISG20 and OASL
 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 Interferon Alpha/beta Signaling Pathway antibodies  Interferon Alpha/beta Signaling Pathway Diagram
Diseases associated with ISG20 elisa kitOrgans/Tissues associated with ISG20 elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >5 publications with ISG20 and Liver Diseases
 Inflammation Antibodies  >3 publications with ISG20 and Inflammation
 Liver Neoplasms Antibodies  >2 publications with ISG20 and Liver Neoplasms
 Carcinoma Antibodies  >2 publications with ISG20 and Carcinoma
 Atherosclerosis Antibodies  >1 publications with ISG20 and Atherosclerosis
 Cardiovascular Diseases Antibodies  >1 publications with ISG20 and Cardiovascular Diseases
 Kidney Neoplasms Antibodies  >1 publications with ISG20 and Kidney Neoplasms
 Disease Models, Animal Antibodies  >1 publications with ISG20 and Disease Models, Animal
 Cell Transformation, Neoplastic Antibodies  >1 publications with ISG20 and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >1 publications with ISG20 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >13 publications with ISG20 and Blood
 Liver Antibodies  >6 publications with ISG20 and Liver
 Uterus Antibodies  >4 publications with ISG20 and Uterus
 Placenta Antibodies  >2 publications with ISG20 and Placenta
 Kidney Antibodies  >2 publications with ISG20 and Kidney
 Brain Antibodies  >2 publications with ISG20 and Brain
 Embryonic Tissue Antibodies  >2 publications with ISG20 and Embryonic Tissue
 Ovary Antibodies  >1 publications with ISG20 and Ovary
 Connective Tissue Antibodies  >1 publications with ISG20 and Connective Tissue
 Heart Antibodies  >1 publications with ISG20 and Heart
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