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IL1b elisa kit :: Human Interleukin 1 Beta (IL1b) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS454363
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $265 +1 FREE 8GB USB
96-Strip-Wells  /  $325 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,410 +1 FREE 8GB USB
10x96-Strip-Wells  /  $2,735 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 1 Beta (IL1b), ELISA Kit

★Popular Item★
 Also Known As   

Human Interleukin 1 Beta (IL1b) ELISA Kit

 Product Synonym Names    IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
 Product Gene Name   

IL1b elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    K02770 mRNA
 3D Structure    ModBase 3D Structure for P01584
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IL1b. No significant cross-reactivity or interference between IL1b and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    31.25-2000pg/mL
 Sensitivity    12.8pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL1b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL1b were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL1b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL1b purchase    MBS454363 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 1 Beta (IL1b) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing IL1b, with an estimated sensitivity of 12.8pg/mL. The ELISA analytical biochemical technique of the MBS454363 kit is based on IL1b antibody-IL1b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL1b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL1b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL1b elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1b in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Standard Curve/Testing Data of IL1b elisa kit    IL1b elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS454363. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL1b. It may not necessarily be applicable to this product.
 NCBI GI #    10835145
 NCBI GeneID    3553
 NCBI Accession #    NP_000567.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000576.2 [Other Products]
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 UniProt Primary Accession #    P01584 [Other Products]
 UniProt Secondary Accession #    Q53X59; Q53XX2; Q7M4S7; Q7RU01; Q96HE5; Q9UCT6 [Other Products]
 UniProt Related Accession #    P01584 [Other Products]
 Molecular Weight    30,748 Da
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 NCBI Official Full Name    interleukin-1 beta proprotein
 NCBI Official Synonym Full Names    interleukin 1 beta
 NCBI Official Symbol    IL1B [Similar Products]
 NCBI Official Synonym Symbols   
IL-1; IL1F2; IL1-BETA
[Similar Products]
 NCBI Protein Information    interleukin-1 beta
 UniProt Protein Name    Interleukin-1 beta
 UniProt Synonym Protein Names   
Catabolin
 Protein Family    Interleukin
 UniProt Gene Name    IL1B [Similar Products]
 UniProt Synonym Gene Names    IL1F2; IL-1 beta [Similar Products]
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 NCBI Summary for IL1b    The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL1b    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
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 Research Articles on IL1b    1. There were no significant differences between patients with Behcet's disease and controls with respect to the IL-1Ra, IL-1beta, IL-2, IL-6 and the IL-10 gene polymorphism distributions. Correlations between the articular involvement and IL-1RN, the ocular involvement and the IL-1beta, and the age of disease onset and the IL-2 and IL-10 polymorphisms suggest that that they could be associated with disease symptoms.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL1b elisa kitPathways associated with IL1b elisa kit
 Reference Product  PubMed Publications
 IL1A elisa kit  >250 publications with IL1b and IL1A
 JUN elisa kit  >203 publications with IL1b and JUN
 CASP1 elisa kit  >186 publications with IL1b and CASP1
 IL18 elisa kit  >89 publications with IL1b and IL18
 IL1R1 elisa kit  >52 publications with IL1b and IL1R1
 NFKB1 elisa kit  >37 publications with IL1b and NFKB1
 FOS elisa kit  >20 publications with IL1b and FOS
 IL1R2 elisa kit  >17 publications with IL1b and IL1R2
 IL1RAP elisa kit  >5 publications with IL1b and IL1RAP
 MAPK14 elisa kit  >4 publications with IL1b and MAPK14
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Diseases associated with IL1b elisa kitOrgans/Tissues associated with IL1b elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >984 publications with IL1b and Inflammation
 Necrosis Antibodies  >647 publications with IL1b and Necrosis
 Disease Models, Animal Antibodies  >278 publications with IL1b and Disease Models, Animal
 Skin Diseases Antibodies  >109 publications with IL1b and Skin Diseases
 Arthritis, Rheumatoid Antibodies  >61 publications with IL1b and Arthritis, Rheumatoid
 Hypersensitivity Antibodies  >51 publications with IL1b and Hypersensitivity
 Stomach Neoplasms Antibodies  >50 publications with IL1b and Stomach Neoplasms
 Colitis Antibodies  >46 publications with IL1b and Colitis
 Asthma Antibodies  >39 publications with IL1b and Asthma
 Myocardial Ischemia Antibodies  >38 publications with IL1b and Myocardial Ischemia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >748 publications with IL1b and Blood
 Brain Antibodies  >206 publications with IL1b and Brain
 Lung Antibodies  >169 publications with IL1b and Lung
 Vascular Antibodies  >139 publications with IL1b and Vascular
 Liver Antibodies  >111 publications with IL1b and Liver
 Skin Antibodies  >97 publications with IL1b and Skin
 Bone Marrow Antibodies  >86 publications with IL1b and Bone Marrow
 Uterus Antibodies  >82 publications with IL1b and Uterus
 Muscle Antibodies  >77 publications with IL1b and Muscle
 Connective Tissue Antibodies  >66 publications with IL1b and Connective Tissue
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