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IRAK4 elisa kit :: Human Interleukin 1 Receptor Associated Kinase 4 (IRAK4) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023519 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $260 +1 FREE 8GB USB
48-Strip-Wells  /  $475 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,590 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,670 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 1 Receptor Associated Kinase 4 (IRAK4), ELISA Kit

 Also Known As   

Interleukin 1 Receptor Associated Kinase 4 (IRAK4) ELISA Kit

 Product Synonym Names    NY-REN-64
 Product Gene Name   

IRAK4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606883
 3D Structure    ModBase 3D Structure for Q9NWZ3
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 4 (IRAK4).
No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 4 (IRAK4) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    1.56-100ng/mL
 Sensitivity    < 0.64ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 4 (IRAK4) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 4 (IRAK4) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IRAK4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IRAK4 purchase    MBS2023519 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 1 Receptor Associated Kinase 4 (IRAK4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56-100ng/mL in biological research samples containing IRAK4, with an estimated sensitivity of < 0.64ng/mL. The ELISA analytical biochemical technique of the MBS2023519 kit is based on IRAK4 antibody-IRAK4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IRAK4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IRAK4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IRAK4 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 4 (IRAK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 4 (IRAK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for IRAK4 elisa kit    Signal transduction; Enzyme & Kinase; Apoptosis; Tumor immunity; Infection immunity; Pulmonology
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 Typical Testing Data/Standard Curve (for reference only) of IRAK4 elisa kit    IRAK4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023519. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IRAK4. It may not necessarily be applicable to this product.
 NCBI GI #    20219010
 NCBI GeneID    51135
 NCBI Accession #    AAM15772.1 [Other Products]
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 UniProt Primary Accession #    Q9NWZ3 [Other Products]
 UniProt Secondary Accession #    Q69FE1; Q8TDF7; Q9Y589 [Other Products]
 UniProt Related Accession #    Q9NWZ3 [Other Products]
 Molecular Weight    37,674 Da
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 NCBI Official Full Name    interleukin-1 receptor associated kinase 4
 NCBI Official Synonym Full Names    interleukin-1 receptor-associated kinase 4
 NCBI Official Symbol    IRAK4 [Similar Products]
 NCBI Official Synonym Symbols   
IPD1; REN64; IRAK-4; NY-REN-64
[Similar Products]
 NCBI Protein Information    interleukin-1 receptor-associated kinase 4
 UniProt Protein Name    Interleukin-1 receptor-associated kinase 4
 UniProt Synonym Protein Names   
Renal carcinoma antigen NY-REN-64
 Protein Family    Interleukin-1 receptor-associated kinase
 UniProt Gene Name    IRAK4 [Similar Products]
 UniProt Synonym Gene Names    IRAK-4 [Similar Products]
 UniProt Entry Name    IRAK4_HUMAN
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 NCBI Summary for IRAK4    This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
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 UniProt Comments for IRAK4    IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: extracellular space; cytoplasm; plasma membrane; endosome membrane; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of smooth muscle cell proliferation; cytokine and chemokine mediated signaling pathway; cytokine production; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency
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 Research Articles on IRAK4    1. Studies indicate that interleukin-1 receptor-associated kinase 4 protein (IRAK4), a serine/threonine kinase, plays a key role in both inflammation and oncology diseases.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IRAK4 elisa kitPathways associated with IRAK4 elisa kit
 Reference Product  PubMed Publications
 IRAK2 elisa kit  >191 publications with IRAK4 and IRAK2
 MYD88 elisa kit  >113 publications with IRAK4 and MYD88
 IRAK1 elisa kit  >68 publications with IRAK4 and IRAK1
 TRAF6 elisa kit  >46 publications with IRAK4 and TRAF6
 IRAK3 elisa kit  >11 publications with IRAK4 and IRAK3
 TIRAP elisa kit  >7 publications with IRAK4 and TIRAP
 PELI1 elisa kit  >5 publications with IRAK4 and PELI1
 PELI2 elisa kit  >1 publications with IRAK4 and PELI2
 IL1R1 elisa kit  >1 publications with IRAK4 and IL1R1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 IL-1 Signaling Pathway antibodies  IL-1 Signaling Pathway Diagram
 IL1-mediated Signaling Events Pathway antibodies  IL1-mediated Signaling Events Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
Diseases associated with IRAK4 elisa kitOrgans/Tissues associated with IRAK4 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >72 publications with IRAK4 and Inflammation
 Necrosis Antibodies  >49 publications with IRAK4 and Necrosis
 Death Antibodies  >38 publications with IRAK4 and Death
 IRAK4 Deficiency Antibodies  >15 publications with IRAK4 and IRAK4 Deficiency
 Autoimmune Diseases Antibodies  >14 publications with IRAK4 and Autoimmune Diseases
 Disease Models, Animal Antibodies  >13 publications with IRAK4 and Disease Models, Animal
 Nervous System Diseases Antibodies  >12 publications with IRAK4 and Nervous System Diseases
 Immunoproliferative Disorders Antibodies  >7 publications with IRAK4 and Immunoproliferative Disorders
 Congenital Abnormalities Antibodies  >5 publications with IRAK4 and Congenital Abnormalities
 Abnormalities, Multiple Antibodies  >5 publications with IRAK4 and Abnormalities, Multiple
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >80 publications with IRAK4 and Blood
 Bone Antibodies  >15 publications with IRAK4 and Bone
 Lung Antibodies  >14 publications with IRAK4 and Lung
 Bone Marrow Antibodies  >12 publications with IRAK4 and Bone Marrow
 Liver Antibodies  >12 publications with IRAK4 and Liver
 Intestine Antibodies  >9 publications with IRAK4 and Intestine
 Brain Antibodies  >9 publications with IRAK4 and Brain
 Kidney Antibodies  >8 publications with IRAK4 and Kidney
 Skin Antibodies  >8 publications with IRAK4 and Skin
 Muscle Antibodies  >7 publications with IRAK4 and Muscle
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