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IL18 elisa kit :: Human Interleukin 18 (IL18) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2701000 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $200 +1 FREE 8GB USB
48-Strip-Wells  /  $310 +1 FREE 8GB USB
96-Strip-Wells  /  $400 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,650 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 18 (IL18), ELISA Kit

★Popular Item★
 Also Known As   

Human Interleukin 18 (IL18) ELISA Kit

 Product Synonym Names    IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma
 Product Gene Name   

IL18 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    D49950 mRNA
 3D Structure    ModBase 3D Structure for Q14116
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Interleukin 18 (IL18).
No significant cross-reactivity or interference between Interleukin 18 (IL18) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Urine, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
 Detection Range    15.6-1000pg/mL
 Sensitivity    < 5.9pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Interleukin 18 (IL18) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Interleukin 18 (IL18) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL18 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL18 purchase    MBS2701000 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 18 (IL18) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6-1000pg/mL in biological research samples containing IL18, with an estimated sensitivity of < 5.9pg/mL. The ELISA analytical biochemical technique of the MBS2701000 kit is based on IL18 antibody-IL18 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL18 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL18. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Urine, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL18 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 (IL18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 18 (IL18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 18 (IL18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 18 (IL18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IL18 elisa kit    IL18 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2701000. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL18. It may not necessarily be applicable to this product.
 NCBI GI #    4504653
 NCBI GeneID    3606
 NCBI Accession #    NP_001553.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001562.3 [Other Products]
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 UniProt Primary Accession #    Q14116 [Other Products]
 UniProt Secondary Accession #    O75599; Q6FGY3; Q6WWJ7 [Other Products]
 UniProt Related Accession #    Q14116 [Other Products]
 Molecular Weight    21,896 Da
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 NCBI Official Full Name    interleukin-18 isoform 1
 NCBI Official Synonym Full Names    interleukin 18
 NCBI Official Symbol    IL18 [Similar Products]
 NCBI Official Synonym Symbols   
IGIF; IL-18; IL-1g; IL1F4
[Similar Products]
 NCBI Protein Information    interleukin-18
 UniProt Protein Name    Interleukin-18
 UniProt Synonym Protein Names   
Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma
 Protein Family    Interleukin
 UniProt Gene Name    IL18 [Similar Products]
 UniProt Synonym Gene Names    IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma [Similar Products]
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 NCBI Summary for IL18    The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
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 UniProt Comments for IL18    Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
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 Research Articles on IL18    1. abnormal IL-18 expression is induced by genital infection and induces damage to male reproductive capacity, thereby causing male infertility
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL18 elisa kitPathways associated with IL18 elisa kit
 Reference Product  PubMed Publications
 CASP1 elisa kit  >537 publications with IL18 and CASP1
 IL10 elisa kit  >302 publications with IL18 and IL10
 IL18R1 elisa kit  >222 publications with IL18 and IL18R1
 IL13 elisa kit  >91 publications with IL18 and IL13
 IL6 elisa kit  >75 publications with IL18 and IL6
 IL18RAP elisa kit  >37 publications with IL18 and IL18RAP
 IL1B elisa kit  >34 publications with IL18 and IL1B
 IFNG elisa kit  >24 publications with IL18 and IFNG
 IL18BP elisa kit  >19 publications with IL18 and IL18BP
 CCR5 elisa kit  >18 publications with IL18 and CCR5
 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cellular Roles Of Anthrax Toxin Pathway antibodies  Cellular Roles Of Anthrax Toxin Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Hypertrophy Model Pathway antibodies  Hypertrophy Model Pathway Diagram
Diseases associated with IL18 elisa kitOrgans/Tissues associated with IL18 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1244 publications with IL18 and Inflammation
 Necrosis Antibodies  >813 publications with IL18 and Necrosis
 Disease Models, Animal Antibodies  >420 publications with IL18 and Disease Models, Animal
 Lung Diseases Antibodies  >332 publications with IL18 and Lung Diseases
 Kidney Diseases Antibodies  >318 publications with IL18 and Kidney Diseases
 Hypersensitivity Antibodies  >271 publications with IL18 and Hypersensitivity
 Arthritis, Rheumatoid Antibodies  >222 publications with IL18 and Arthritis, Rheumatoid
 Heart Diseases Antibodies  >211 publications with IL18 and Heart Diseases
 Hypersensitivity, Immediate Antibodies  >191 publications with IL18 and Hypersensitivity, Immediate
 Atherosclerosis Antibodies  >162 publications with IL18 and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >419 publications with IL18 and Liver
 Lung Antibodies  >321 publications with IL18 and Lung
 Heart Antibodies  >226 publications with IL18 and Heart
 Brain Antibodies  >220 publications with IL18 and Brain
 Skin Antibodies  >220 publications with IL18 and Skin
 Bone Antibodies  >218 publications with IL18 and Bone
 Intestine Antibodies  >165 publications with IL18 and Intestine
 Bone Marrow Antibodies  >146 publications with IL18 and Bone Marrow
 Muscle Antibodies  >137 publications with IL18 and Muscle
 Connective Tissue Antibodies  >76 publications with IL18 and Connective Tissue
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