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IL25 elisa kit :: Human Interleukin 25 (IL25) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS454328 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $320 +1 FREE 8GB USB
96-Strip-Wells  /  $420 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,860 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,595 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 25 (IL25), ELISA Kit

★Popular Item★
 Also Known As   

Human Interleukin 25 (IL25) ELISA Kit

 Product Synonym Names    IL17E; IL17-E; Interleukin-17E
 Product Gene Name   

IL25 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    AF305200 mRNA
 3D Structure    ModBase 3D Structure for Q9H293
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IL25. No significant cross-reactivity or interference between IL25 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    15.62-1000pg/mL
 Sensitivity    5.67pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL25 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL25 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL25 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL25 purchase    MBS454328 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 25 (IL25) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.62-1000pg/mL in biological research samples containing IL25, with an estimated sensitivity of 5.67pg/mL. The ELISA analytical biochemical technique of the MBS454328 kit is based on IL25 antibody-IL25 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL25 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL25. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL25 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL25 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL25. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IL25 elisa kit    IL25 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS454328. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL25. It may not necessarily be applicable to this product.
 NCBI GI #    12232485
 NCBI GeneID    64806
 NCBI Accession #    NP_073626.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_022789.3 [Other Products]
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 UniProt Primary Accession #    Q9H293 [Other Products]
 UniProt Secondary Accession #    Q2M3F0; Q8IZV3; Q8WXB0 [Other Products]
 UniProt Related Accession #    Q9H293 [Other Products]
 Molecular Weight    18,523 Da
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 NCBI Official Full Name    interleukin-25 isoform 1
 NCBI Official Synonym Full Names    interleukin 25
 NCBI Official Symbol    IL25 [Similar Products]
 NCBI Official Synonym Symbols   
IL17E
[Similar Products]
 NCBI Protein Information    interleukin-25
 UniProt Protein Name    Interleukin-25
 UniProt Synonym Protein Names   
Interleukin-17E; IL-17E
 Protein Family    Interleukin
 UniProt Gene Name    IL25 [Similar Products]
 UniProt Synonym Gene Names    IL17E; IL-25; IL-17E [Similar Products]
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 NCBI Summary for IL25    The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
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 UniProt Comments for IL25    Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
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 Research Articles on IL25    1. Increased induction and expression of TSLP, IL-25, and IL-33 from nasal epithelial cells contribute to the pathophysiology of eosinophilic chronic rhinosinusitis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL25 elisa kitPathways associated with IL25 elisa kit
 Reference Product  PubMed Publications
 TSLP elisa kit  >14 publications with IL25 and TSLP
 IL17A elisa kit  >10 publications with IL25 and IL17A
 IL17RB elisa kit  >6 publications with IL25 and IL17RB
 IL17RA elisa kit  >3 publications with IL25 and IL17RA
 TRAF3IP2 elisa kit  >2 publications with IL25 and TRAF3IP2
 GATA3 elisa kit  >2 publications with IL25 and GATA3
 IL17RC elisa kit  >1 publications with IL25 and IL17RC
 SOCS3 elisa kit  >1 publications with IL25 and SOCS3
 C19orf10 elisa kit  >1 publications with IL25 and C19orf10
 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Development And Heterogeneity Of The ILC Family Pathway antibodies  Development And Heterogeneity Of The ILC Family Pathway Diagram
 IL17 Signaling Pathway antibodies  IL17 Signaling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interleukin-17 Signaling Pathway antibodies  Interleukin-17 Signaling Pathway Diagram
 Signaling By Interleukins Pathway antibodies  Signaling By Interleukins Pathway Diagram
Diseases associated with IL25 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >47 publications with IL25 and Inflammation
 Hypersensitivity Antibodies  >40 publications with IL25 and Hypersensitivity
 Asthma Antibodies  >33 publications with IL25 and Asthma
 Lung Diseases Antibodies  >28 publications with IL25 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >23 publications with IL25 and Respiratory Hypersensitivity
 Hyperplasia Antibodies  >10 publications with IL25 and Hyperplasia
 Disease Models, Animal Antibodies  >8 publications with IL25 and Disease Models, Animal
 Necrosis Antibodies  >5 publications with IL25 and Necrosis
 Fibrosis Antibodies  >3 publications with IL25 and Fibrosis
 Death Antibodies  >3 publications with IL25 and Death
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