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IL-28B elisa kit :: Human Interleukin 28B (IL-28B) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700232
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 28B (IL-28B), ELISA Kit

★Popular Item★
 Also Known As   

Human Interleukin 28B, IL-28B ELISA Kit

 Product Synonym Names    Human Interleukin 28B (IL-28B) ELISA kit; IFNL3; IL-28B; IL28C; interferon; lambda 3; interferon; lambda 4; interleukin 28B; interleukin 28C; Interleukin 28B (IL-28B)
 Product Gene Name   

IL-28B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700232 Testing Data    Testing Data PDF
 OMIM    607402
 3D Structure    ModBase 3D Structure for Q8IZI9
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human IL-28B. No significant cross-reactivity or interference between human IL-28B and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    1.56 pg/ml-100 pg/ml.
 Sensitivity    0.39 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-28B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-28B purchase    MBS700232 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 28B (IL-28B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 pg/ml-100 pg/ml in biological research samples containing IL-28B, with an estimated sensitivity of 0.39 pg/ml. The ELISA analytical biochemical technique of the MBS700232 kit is based on IL-28B antibody-IL-28B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-28B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-28B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL-28B elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-28B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-28B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-28B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-28B bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of IL-28B elisa kit    IL-28B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700232. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-28B. It may not necessarily be applicable to this product.
 NCBI GI #    28144901
 NCBI GeneID    282617
 NCBI Accession #    NP_742151.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_172139.2 [Other Products]
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 UniProt Primary Accession #    Q8IZI9 [Other Products]
 UniProt Secondary Accession #    Q6VN56; Q7Z4J3; Q8IWL6; A2BDE1 [Other Products]
 UniProt Related Accession #    Q8IZI9 [Other Products]
 Molecular Weight    21,706 Da
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 NCBI Official Full Name    interferon lambda-3
 NCBI Official Synonym Full Names    interferon, lambda 3
 NCBI Official Symbol    IFNL3 [Similar Products]
 NCBI Official Synonym Symbols   
IL28B; IL28C; IL-28B
[Similar Products]
 NCBI Protein Information    interferon lambda-3; IL-28C; IFN-lambda-3; IFN-lambda-4; interleukin 28C; interleukin-28B; interleukin-28C; cytokine Zcyto22; interferon lambda-4; interferon, lambda 4; interleukin 28B (interferon, lambda 3)
 UniProt Protein Name    Interferon lambda-3
 UniProt Synonym Protein Names   
Cytokine Zcyto22; Interleukin-28B; IL-28B; Interleukin-28C; IL-28C
 UniProt Gene Name    IFNL3 [Similar Products]
 UniProt Synonym Gene Names    IL28B; IL28C; ZCYTO22; IFN-lambda-3; IL-28B; IL-28C [Similar Products]
 UniProt Entry Name    IFNL3_HUMAN
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 NCBI Summary for IL-28B    This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL-28B    Function: Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
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 Research Articles on IL-28B    1. could not find any difference in the predictive impact of any of three SNPs of IFNL4 or IL-28B with regard to susceptibility to HCV or treatment response
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-28B elisa kitPathways associated with IL-28B elisa kit
 Reference Product  PubMed Publications
 IFNA2 elisa kit  >5 publications with IL-28B and IFNA2
 ITPA elisa kit  >1 publications with IL-28B and ITPA
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Type III Interferon Signaling Pathway antibodies  Type III Interferon Signaling Pathway Diagram
Diseases associated with IL-28B elisa kit
 Disease Name  Pubmed Publications
 Hepatitis C Antibodies  >26 publications with IL-28B and Hepatitis C
 Hepatitis, Chronic Antibodies  >20 publications with IL-28B and Hepatitis, Chronic
 Hepatitis C, Chronic Antibodies  >11 publications with IL-28B and Hepatitis C, Chronic
 Hepatitis B, Chronic Antibodies  >3 publications with IL-28B and Hepatitis B, Chronic
 Liver Cirrhosis Antibodies  >2 publications with IL-28B and Liver Cirrhosis
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