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IL 32a elisa kit :: Human Interleukin 32 Alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS750027 (PROMOTIONAL PRICING: 10% OFF web price. Discount is not applicable for bulk pricing such as 5x96T or 10x96T. Discount will be applied after order has been placed)
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 32 Alpha (IL 32a), ELISA Kit

★Popular Item★
 Also Known As   

Human Interleukin 32 Alpha ELISA Kit

 Product Gene Name   

IL 32a elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS750027 COA    COA PDF
 OMIM    606001
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IL-32alpha. No significant cross-reactivity or interference between IL-32alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-32alpha and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL 32a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL 32a purchase    MBS750027 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 32 Alpha (IL 32a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing IL 32a, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS750027 kit is based on IL 32a antibody-IL 32a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL 32a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL 32a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL 32a elisa kit

   Intended Uses: This IL-32alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL-32alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: IL-32alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-32alpha antibody and an IL-32alpha -HRP conjugate. The assay sample and buffer are incubated together with IL-32alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-32alpha concentration since IL-32alpha from samples and IL-32alpha -HRP conjugate compete for the anti-IL-32alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-32alpha from the sample, fewer sites are left to bind IL-32alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-32alpha concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IL 32a elisa kit    Immunology
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 Typical Standard Curve/Testing Data of IL 32a elisa kit    IL 32a elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS750027. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL 32a. It may not necessarily be applicable to this product.
 NCBI GI #    61658634
 NCBI GeneID    9235
 NCBI Accession #    NP_001012651.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001012633.1 [Other Products]
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 UniProt Secondary Accession #    Q5VFH7; Q5VFH8; Q8WV38; Q96GK9; A6NNM0; A8MPX0; B4DJM1; B8Q191; D3DUB0; D3DUB2 [Other Products]
 UniProt Related Accession #    P24001 [Other Products]
 Molecular Weight    17,003 Da
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 NCBI Official Full Name    interleukin-32 isoform A
 NCBI Official Synonym Full Names    interleukin 32
 NCBI Official Symbol    IL32 [Similar Products]
 NCBI Official Synonym Symbols   
NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
[Similar Products]
 NCBI Protein Information    interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cells protein 4; natural killer cell transcript 4; tumor necrosis factor alpha-inducing factor
 UniProt Protein Name    Interleukin-32
 UniProt Synonym Protein Names   
Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor
 UniProt Gene Name    IL32 [Similar Products]
 UniProt Synonym Gene Names    NK4; TAIF; IL-32 [Similar Products]
 UniProt Entry Name    IL32_HUMAN
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 NCBI Summary for IL 32a    This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for IL 32a    IL32: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF- alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular space; membrane

Molecular Function: protein binding; cytokine activity

Biological Process: immune response; defense response; cell adhesion
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 Research Articles on IL 32a    1. Overexpression of IL32 is associated with head and neck squamous cell carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL 32a elisa kitDiseases associated with IL 32a elisa kit
 Reference Product  PubMed Publications
 PRTN3 elisa kit  >6 publications with IL 32a and PRTN3
 IL18 elisa kit  >5 publications with IL 32a and IL18
 PTGS2 elisa kit  >2 publications with IL 32a and PTGS2
 IL1B elisa kit  >2 publications with IL 32a and IL1B
 CXCL2 elisa kit  >1 publications with IL 32a and CXCL2
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >65 publications with IL 32a and Inflammation
 Necrosis Antibodies  >51 publications with IL 32a and Necrosis
 Immune System Diseases Antibodies  >33 publications with IL 32a and Immune System Diseases
 Lung Diseases Antibodies  >14 publications with IL 32a and Lung Diseases
 Vascular Diseases Antibodies  >12 publications with IL 32a and Vascular Diseases
 Carcinoma Antibodies  >11 publications with IL 32a and Carcinoma
 Nervous System Diseases Antibodies  >9 publications with IL 32a and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >9 publications with IL 32a and Cardiovascular Diseases
 Liver Diseases Antibodies  >7 publications with IL 32a and Liver Diseases
 Lung Neoplasms Antibodies  >6 publications with IL 32a and Lung Neoplasms
Organs/Tissues associated with IL 32a elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >62 publications with IL 32a and Blood
 Lung Antibodies  >18 publications with IL 32a and Lung
 Vascular Antibodies  >15 publications with IL 32a and Vascular
 Bone Antibodies  >10 publications with IL 32a and Bone
 Intestine Antibodies  >9 publications with IL 32a and Intestine
 Liver Antibodies  >8 publications with IL 32a and Liver
 Bone Marrow Antibodies  >7 publications with IL 32a and Bone Marrow
 Stomach Antibodies  >6 publications with IL 32a and Stomach
 Lymph Node Antibodies  >6 publications with IL 32a and Lymph Node
 Brain Antibodies  >4 publications with IL 32a and Brain
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