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IL6 elisa kit :: Human Interleukin 6 (IL6) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021124 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $225 +1 FREE 8GB USB
48-Strip-Wells  /  $375 +1 FREE 8GB USB
96-Strip-Wells  /  $495 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,970 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,550 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 6 (IL6), ELISA Kit

★Popular Item★
 Also Known As   

Interleukin 6 (IL6) ELISA Kit

 Product Synonym Names    MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
 Product Gene Name   

IL6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    147620
 3D Structure    ModBase 3D Structure for P05231
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6).
No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    7.8-500pg/mL
 Sensitivity    < 3.2pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL6 purchase    MBS2021124 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 6 (IL6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8-500pg/mL in biological research samples containing IL6, with an estimated sensitivity of < 3.2pg/mL. The ELISA analytical biochemical technique of the MBS2021124 kit is based on IL6 antibody-IL6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL6 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for IL6 elisa kit    Cytokine; Tumor immunity; Infection immunity; Cardiovascular biology
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 Typical Testing Data/Standard Curve (for reference only) of IL6 elisa kit    IL6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021124. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL6. It may not necessarily be applicable to this product.
 NCBI GI #    10834984
 NCBI GeneID    3569
 NCBI Accession #    NP_000591.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000600.3 [Other Products]
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 UniProt Primary Accession #    P05231 [Other Products]
 UniProt Secondary Accession #    Q9UCU2; Q9UCU3; Q9UCU4 [Other Products]
 UniProt Related Accession #    P05231 [Other Products]
 Molecular Weight    23,718 Da
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 NCBI Official Full Name    interleukin-6
 NCBI Official Synonym Full Names    interleukin 6
 NCBI Official Symbol    IL6 [Similar Products]
 NCBI Official Synonym Symbols   
HGF; HSF; BSF2; IL-6; IFNB2
[Similar Products]
 NCBI Protein Information    interleukin-6
 UniProt Protein Name    Interleukin-6
 UniProt Synonym Protein Names   
B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
 Protein Family    Interleukin
 UniProt Gene Name    IL6 [Similar Products]
 UniProt Synonym Gene Names    IFNB2; IL-6; BSF-2; CDF; IFN-beta-2 [Similar Products]
 UniProt Entry Name    IL6_HUMAN
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 NCBI Summary for IL6    This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
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 UniProt Comments for IL6    IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p21

Cellular Component: extracellular space; cytoplasm; extracellular region; interleukin-6 receptor complex; external side of plasma membrane

Molecular Function: protein binding; interleukin-6 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; negative regulation of cytokine secretion; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; bone remodeling; glucose homeostasis; activation of NF-kappaB transcription factor; positive regulation of tyrosine phosphorylation of Stat3 protein; response to caffeine; muscle maintenance; response to antibiotic; regulation of cell shape; monocyte chemotaxis; positive regulation of T-helper 2 cell differentiation; positive regulation of acute inflammatory response; negative regulation of gluconeogenesis; acute-phase response; positive regulation of T cell proliferation; cell growth; response to electrical stimulus; defense response to virus; neurite development; response to drug; platelet activation; positive regulation of protein import into nucleus, translocation; positive regulation of interleukin-6 production; defense response to protozoan; negative regulation of fat cell differentiation; response to amino acid stimulus; positive regulation of chemokine production; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; cell redox homeostasis; response to heat; positive regulation of B cell activation; neutrophil apoptosis; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; response to calcium ion; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of muscle development; positive regulation of translation; negative regulation of hormone secretion; positive regulation of smooth muscle cell proliferation; neutrophil mediated immunity; negative regulation of caspase activity; regulation of circadian sleep/wake cycle, non-REM sleep; defense response to Gram-negative bacterium; response to insulin stimulus; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to peptidoglycan; positive regulation of cell proliferation; hepatic immune response; inflammatory response; aging; negative regulation of chemokine biosynthetic process; response to nutrient levels; cytokine and chemokine mediated signaling pathway; positive regulation of immunoglobulin secretion; positive regulation of peptidyl-serine phosphorylation; regulation of angiogenesis; endocrine pancreas development; humoral immune response; positive regulation of protein kinase B signaling cascade; positive regulation of osteoblast differentiation; response to cold; positive regulation of neuron differentiation; positive regulation of DNA replication; positive regulation of transmission of nerve impulse

Disease: Rheumatoid Arthritis, Systemic Juvenile; Arteriovenous Malformations Of The Brain; Kaposi Sarcoma, Susceptibility To; Inflammatory Bowel Disease 1
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 Research Articles on IL6    1. Data support a model in which EWS/FLI1 deficiency results in the secretion of soluble factors, such as IL6, which activate STAT3 in bystander cells that maintain EWS/FLI1 expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL6 elisa kitPathways associated with IL6 elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >585 publications with IL6 and JUN
 STAT3 elisa kit  >382 publications with IL6 and STAT3
 IL1B elisa kit  >280 publications with IL6 and IL1B
 IL17A elisa kit  >118 publications with IL6 and IL17A
 IL6R elisa kit  >117 publications with IL6 and IL6R
 JAK2 elisa kit  >65 publications with IL6 and JAK2
 STAT1 elisa kit  >61 publications with IL6 and STAT1
 SOCS3 elisa kit  >54 publications with IL6 and SOCS3
 IL6ST elisa kit  >46 publications with IL6 and IL6ST
 FOS elisa kit  >43 publications with IL6 and FOS
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
Diseases associated with IL6 elisa kitOrgans/Tissues associated with IL6 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2361 publications with IL6 and Inflammation
 Necrosis Antibodies  >1714 publications with IL6 and Necrosis
 Autoimmune Diseases Antibodies  >447 publications with IL6 and Autoimmune Diseases
 Skin Diseases Antibodies  >290 publications with IL6 and Skin Diseases
 Obesity Antibodies  >289 publications with IL6 and Obesity
 Arthritis, Rheumatoid Antibodies  >264 publications with IL6 and Arthritis, Rheumatoid
 Atherosclerosis Antibodies  >243 publications with IL6 and Atherosclerosis
 Myocardial Ischemia Antibodies  >226 publications with IL6 and Myocardial Ischemia
 Ischemia Antibodies  >117 publications with IL6 and Ischemia
 Diabetes Mellitus, Type 2 Antibodies  >108 publications with IL6 and Diabetes Mellitus, Type 2
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3006 publications with IL6 and Blood
 Vascular Antibodies  >664 publications with IL6 and Vascular
 Bone Antibodies  >408 publications with IL6 and Bone
 Lung Antibodies  >394 publications with IL6 and Lung
 Brain Antibodies  >371 publications with IL6 and Brain
 Muscle Antibodies  >295 publications with IL6 and Muscle
 Kidney Antibodies  >269 publications with IL6 and Kidney
 Connective Tissue Antibodies  >206 publications with IL6 and Connective Tissue
 Skin Antibodies  >182 publications with IL6 and Skin
 Eye Antibodies  >124 publications with IL6 and Eye
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